BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,128,786	T→A	5.8%	P84P (CCT→CCA)	yhbW →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,128,786	0	T	A	5.8%	297.9 / 7.7	104	P84P (CCT→CCA)	yhbW	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (36/62); new base A (4/2); total (40/64)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 108 total aligned reads displayed.

GGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGCGAATCGAT  >  minE/2128717‑2128855

ggTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCt                                                                       <  1:471531/70‑1 (MQ=255)

ggTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCt                                                                       >  1:4004748/1‑70 (MQ=255)

ggTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCt                                                                       <  1:5343502/70‑1 (MQ=255)

ggTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCt                                                                       <  1:2933546/70‑1 (MQ=255)

ggTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGGCTGGCGGCGTGATGTTGCCTa                                                                      <  1:3758127/71‑1 (MQ=255)

 gTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTaa                                                                     >  1:3096037/1‑71 (MQ=255)

    ttGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCa                                                                  >  1:4240667/1‑71 (MQ=255)

     tGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCAc                                                                 >  1:1044405/1‑71 (MQ=255)

        tCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAAc                                                                    <  1:150958/65‑1 (MQ=255)

        tCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAAc                                                                    <  1:199427/65‑1 (MQ=255)

        tCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCa                                                              >  1:3250422/1‑71 (MQ=255)

         cGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCAc                                                             >  1:2852645/1‑71 (MQ=255)

         cGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCAc                                                             >  1:1542697/1‑71 (MQ=255)

          ggCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCAc                                                             <  1:3175396/70‑1 (MQ=255)

           gCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCg                                                           >  1:4809070/1‑71 (MQ=255)

           gCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCg                                                           >  1:5033214/1‑71 (MQ=255)

              aTCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTg                                                        >  1:2881571/1‑71 (MQ=255)

              aTCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTg                                                        >  1:2887994/1‑71 (MQ=255)

                 tGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGt                                                      >  1:5604197/1‑70 (MQ=255)

                  ggcggcGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCa                                                    >  1:1855188/1‑71 (MQ=255)

                  ggcggcGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCa                                                    >  1:757079/1‑71 (MQ=255)

                  ggcggcGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCa                                                    >  1:2269511/1‑71 (MQ=255)

                  ggcggcGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCa                                                    >  1:3135834/1‑71 (MQ=255)

                   gcggcgAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTTGTc                                                     <  1:2030040/69‑1 (MQ=255)

                   gcggcaAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTACCAAACCACTCACCGTTGGtg                                                     <  1:2111076/69‑2 (MQ=255)

                   gcggcaAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTACCAAACCACTCACCGTTGGtg                                                     <  1:1155315/69‑2 (MQ=255)

                    cggcgAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCAtt                                                  <  1:3205555/71‑1 (MQ=255)

                        gAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                              <  1:2432677/71‑1 (MQ=255)

                        gAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                              <  1:3612360/71‑1 (MQ=255)

                        gAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                              <  1:3690774/71‑1 (MQ=255)

                         aaTACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                              >  1:1195383/1‑70 (MQ=255)

                         aaTACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGa                                             <  1:5525017/71‑1 (MQ=255)

                          aTACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGt                                                      <  1:4987983/61‑1 (MQ=255)

                          aTACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGaa                                            >  1:1282772/1‑71 (MQ=255)

                           tACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGt                                                      <  1:4974268/60‑1 (MQ=255)

                               accacGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGtt                                       >  1:200472/1‑71 (MQ=255)

                                   cGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCgg                                    >  1:3442286/1‑70 (MQ=255)

                                        cATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                              <  1:2755949/55‑1 (MQ=255)

                                        cATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                              <  1:4171005/55‑1 (MQ=255)

                                        cATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGc                               <  1:2109652/70‑1 (MQ=255)

                                        cATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGc                               <  1:5077610/70‑1 (MQ=255)

                                        cATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGCTCATTGCAg                                              <  1:2160332/55‑1 (MQ=255)

                                         aTCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                             <  1:5173617/71‑1 (MQ=255)

                                         aTCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                             <  1:1416187/71‑1 (MQ=255)

                                         aTCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                             <  1:5563804/71‑1 (MQ=255)

                                         aTCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                             <  1:4520205/71‑1 (MQ=255)

                                         aTCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCt                              >  1:725394/1‑70 (MQ=255)

                                          tCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTTGTCATTGCAg                                              <  1:820875/53‑1 (MQ=255)

                                          tCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                              <  1:3186282/53‑1 (MQ=255)

                                          tCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                              <  1:1805496/53‑1 (MQ=255)

                                          tCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                              <  1:4622542/53‑1 (MQ=255)

                                          tCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                              <  1:2927221/53‑1 (MQ=255)

                                            tGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAAc                                           >  1:2481454/1‑54 (MQ=255)

                                            tGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                             <  1:4250587/68‑1 (MQ=255)

                                            tGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                             <  1:4631648/68‑1 (MQ=255)

                                             ggggTCTGGCGGCGTGATGTTGCCTAACCACTCAcc                                                            <  1:1948425/36‑1 (MQ=255)

                                             ggggTCTGGCGGCGTGATGTTGCCTAACCACTCAcc                                                            <  1:3989837/36‑1 (MQ=255)

                                             ggggTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCt                              <  1:3158381/66‑1 (MQ=255)

                                              gggTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTaa                           <  1:4569475/68‑1 (MQ=255)

                                              gggTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTaa                           <  1:1666822/68‑1 (MQ=255)

                                              gggTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTaa                           <  1:751515/68‑1 (MQ=255)

                                              gggTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTaa                           <  1:3154073/68‑1 (MQ=255)

                                              gggTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATa                         >  1:4770232/1‑70 (MQ=255)

                                                 tCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGtt                                       <  1:2843948/53‑1 (MQ=255)

                                                 tCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGtt                                       <  1:2297250/53‑1 (MQ=255)

                                                 tCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACAct                     <  1:347671/71‑1 (MQ=255)

                                                   tGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                              <  1:5201139/44‑1 (MQ=255)

                                                   tGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                              <  1:1966137/44‑1 (MQ=255)

                                                   tGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCa                                  >  1:386594/1‑56 (MQ=255)

                                                   tGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACActc                    >  1:1214574/1‑70 (MQ=255)

                                                   tGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACActc                    >  1:4533210/1‑70 (MQ=255)

                                                   tGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGCTCGGCACGCtt                             <  1:3654481/61‑1 (MQ=255)

                                                     gcggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAg                                         >  1:3809273/1‑47 (MQ=255)

                                                     gcggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGtt                                       >  1:5268446/1‑49 (MQ=255)

                                                     gcggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGt                                        >  1:5201349/1‑48 (MQ=255)

                                                     gcggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACActct                   <  1:1853371/69‑1 (MQ=255)

                                                     gcggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTa                  >  1:3924417/1‑70 (MQ=255)

                                                      cggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATc                >  1:2422984/1‑71 (MQ=255)

                                                       ggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATcc               <  1:4115837/71‑1 (MQ=255)

                                                         cgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCgg             >  1:2086487/1‑71 (MQ=255)

                                                          gTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCggg            >  1:302183/1‑71 (MQ=255)

                                                           tGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCgggg           >  1:5487209/1‑71 (MQ=255)

                                                            gATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGc          >  1:3159227/1‑71 (MQ=255)

                                                            gATGTTACCAAACCACTCACCGTTGGTGATTGCCGAACAGTTCGGCACGCTCAAt                          >  1:4944276/1‑55 (MQ=37)

                                                            gATGTTACCAAACCACTCACCGTTGGTGATTGCCGAACAGTTCGGCACGCTCAAt                          >  1:4578327/1‑55 (MQ=37)

                                                            gATGTTACCAAACCACTCACCGTTGGTGATTGCCGAACAGTTCGGCACGCTCAAt                          >  1:4435032/1‑55 (MQ=37)

                                                            gATGTTACCAAACCACTCACCGTTGGTGATTGCCGAACAGTTCGGCACGCTCAAt                          >  1:1823771/1‑55 (MQ=37)

                                                              tGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                             <  1:2112398/50‑1 (MQ=255)

                                                              tGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                             <  1:5153639/50‑1 (MQ=255)

                                                              tGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                             <  1:1590835/50‑1 (MQ=255)

                                                              tGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                             <  1:2583454/50‑1 (MQ=255)

                                                              tGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                             <  1:3812922/50‑1 (MQ=255)

                                                                ttGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATa                         >  1:4714484/1‑52 (MQ=255)

                                                                ttGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGCGAAt      <  1:1097033/71‑1 (MQ=255)

                                                                   ccTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGCGAATCg    <  1:564131/70‑1 (MQ=255)

                                                                   ccTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGCGAATCg    <  1:4119494/70‑1 (MQ=255)

                                                                   ccTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGCGAATCg    <  1:3644881/70‑1 (MQ=255)

                                                                   ccTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGCGAATCGa   <  1:5318588/71‑1 (MQ=255)

                                                                   ccTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGCGAATCGa   <  1:2482414/71‑1 (MQ=255)

                                                                    cTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGCGAATCGAt  <  1:3175105/71‑1 (MQ=255)

GGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGCGAATCGAT  >  minE/2128717‑2128855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`