| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,658 | C→G | 9.2% | L405F (TTG→TTC) ‡ | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,658 | 0 | C | G | 9.2% | 230.7 / 10.0 | 76 | L405F (TTG→TTC) ‡ | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (38/31); new base G (3/4); total (41/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
TTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGA > minE/311589‑311728 | ttttGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATccacc > 1:1251386/1‑70 (MQ=255) tGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCaa > 1:1358828/1‑69 (MQ=255) gcggcAAACCTTCTTGCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATccacc > 1:5323789/1‑65 (MQ=255) gcggcAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATccacc > 1:1743876/1‑65 (MQ=255) gcggcAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAgg > 1:4890893/1‑71 (MQ=255) gcggcAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAgg > 1:951509/1‑71 (MQ=255) gcAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGcc > 1:1329531/1‑70 (MQ=255) cAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGa < 1:406510/71‑1 (MQ=255) tcttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg > 1:3077261/1‑71 (MQ=255) tcttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg > 1:109510/1‑71 (MQ=255) cttcCGCCATAACACGCTCAACGTTTTCTACCATAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg > 1:5422651/1‑70 (MQ=255) cttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGat < 1:463952/70‑2 (MQ=255) cttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg < 1:1766026/70‑1 (MQ=255) cttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg < 1:1815962/70‑1 (MQ=255) cttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg < 1:4820727/70‑1 (MQ=255) tcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCg < 1:59304/66‑1 (MQ=255) tcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCg < 1:19042/66‑1 (MQ=255) cGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCTATGGCGAGCACCa < 1:538705/71‑1 (MQ=255) gCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCAt < 1:1433740/71‑1 (MQ=255) ccATAACACGCTCAACGTTATCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg < 1:3258236/64‑1 (MQ=255) aTAACATGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATccc > 1:1056061/1‑71 (MQ=255) aTAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATccc > 1:4990330/1‑71 (MQ=255) aTAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATccc > 1:1644918/1‑71 (MQ=255) tAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCg < 1:3669603/71‑1 (MQ=255) tAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCg < 1:4198635/71‑1 (MQ=255) aaCACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCg > 1:3579065/1‑70 (MQ=255) aaCACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCg > 1:3062090/1‑70 (MQ=255) acacGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGaa > 1:2018696/1‑71 (MQ=255) acacGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGaa < 1:252856/71‑1 (MQ=255) acacGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGaa > 1:5234260/1‑71 (MQ=255) acGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACa > 1:4959289/1‑71 (MQ=255) acGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACa > 1:3007397/1‑71 (MQ=255) acGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACa > 1:2951861/1‑71 (MQ=255) gCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACAtt > 1:4596310/1‑71 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATccacca < 1:3480826/37‑1 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATccacca < 1:3967913/37‑1 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATccacca < 1:4555398/37‑1 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATccacca < 1:2937573/37‑1 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg < 1:2701007/53‑1 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGt > 1:4315453/1‑71 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGt > 1:5357395/1‑71 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGt > 1:1579717/1‑71 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGt > 1:4643062/1‑71 (MQ=255) aCGTTTTCTACCACAACGATGGCGTCATCCACCAACAgg < 1:1445885/39‑1 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCCCCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGc > 1:2161141/1‑71 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATg > 1:2791053/1‑44 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATg > 1:2560903/1‑44 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATg > 1:920907/1‑44 (MQ=255) ttttCTACCACAACGATGGCGTCATCCACCAACAGGCCGa < 1:4518397/40‑1 (MQ=255) ttttCTACCACAACGATGGCGTCATCCACCAACAGGCCGa < 1:1807354/40‑1 (MQ=255) ttttCTACCACAACGATGGCGTCATCCACCAACAGGCCGa < 1:1845934/40‑1 (MQ=255) ttttCTACCACAACGATGGCGTCATACACCAACAGGCCGa < 1:4354943/40‑1 (MQ=255) ttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtgt > 1:5433983/1‑71 (MQ=255) ttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtgt > 1:1397721/1‑71 (MQ=255) ttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtg < 1:4225786/70‑1 (MQ=255) tCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtgt < 1:3700339/70‑1 (MQ=255) cTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACAt < 1:1941279/58‑1 (MQ=255) caccaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGttga > 1:4149736/2‑69 (MQ=9) caccaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGttg < 1:4994313/69‑2 (MQ=9) ccaccaCTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGAtg < 1:477367/71‑2 (MQ=2) ccACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATc > 1:1276196/1‑71 (MQ=255) caccacTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGaa > 1:3822069/1‑51 (MQ=2) caccacTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGAtg < 1:5562986/70‑2 (MQ=2) acaACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCga < 1:2423164/71‑1 (MQ=255) caACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCa > 1:2347169/1‑39 (MQ=255) aaCGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACa > 1:3396943/1‑50 (MQ=255) tGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa < 1:1071670/71‑1 (MQ=255) ggCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa > 1:2725324/1‑70 (MQ=255) gcatCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaa > 1:1607906/4‑70 (MQ=9) gCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa > 1:3622721/1‑69 (MQ=255) gTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg < 1:2435204/71‑1 (MQ=255) gTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg > 1:5396240/1‑71 (MQ=255) gTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg > 1:5049028/1‑71 (MQ=255) tCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGt < 1:1496414/44‑1 (MQ=9) tCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggc < 1:1420939/71‑1 (MQ=255) tCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggc < 1:4854868/71‑1 (MQ=255) aTCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcgg > 1:539611/1‑71 (MQ=255) tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaa < 1:5550556/64‑1 (MQ=255) tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaa < 1:2642586/64‑1 (MQ=255) tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc < 1:5510265/71‑1 (MQ=255) tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcgg > 1:4525407/1‑70 (MQ=255) tCCACCAACAGGCAGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaa < 1:4851666/64‑1 (MQ=255) ccaccaACAGGCCGATGGCGAGCACCATCCCGAACATTg < 1:1978624/39‑1 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGtt > 1:4206916/1‑40 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTGTCGAGAAGCCAAAg > 1:4383075/1‑64 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTAt > 1:2497605/1‑51 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCga > 1:2975313/1‑54 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc > 1:65230/1‑69 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAg > 1:2174257/1‑64 (MQ=255) accaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg < 1:2964428/71‑1 (MQ=255) accaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg < 1:3421285/71‑1 (MQ=255) accaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg < 1:3755918/71‑1 (MQ=255) ccaACAGGCCGATGGCGAGCACCATCCCGAACATTg < 1:3126267/36‑1 (MQ=255) ccaACAGGCCGATGGCGAGCACCATCCCGAACATTg < 1:3975930/36‑1 (MQ=255) ccaACAGGCCGATGGCGAGCACCATCCCGAACATTg < 1:5381640/36‑1 (MQ=255) caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTAt < 1:4071180/48‑1 (MQ=255) caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTAt < 1:540908/48‑1 (MQ=255) caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTAt < 1:5616912/48‑1 (MQ=255) caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCga < 1:510460/51‑1 (MQ=255) caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa > 1:2970675/1‑71 (MQ=255) | TTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGA > minE/311589‑311728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |