| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,130,656 | C→G | 11.1% | L83L (CTG→CTC) ‡ | mtr ← | tryptophan transporter of high affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,130,656 | 0 | C | G | 11.1% | 287.2 / 22.3 | 108 | L83L (CTG→CTC) ‡ | mtr | tryptophan transporter of high affinity |
| Reads supporting (aligned to +/- strand): ref base C (44/51); new base G (5/7); total (50/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
TATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATC > minE/2130586‑2130725 | tataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGccc < 1:2750719/71‑1 (MQ=255) ataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGccc < 1:3340983/70‑1 (MQ=255) taGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAg > 1:17965/1‑71 (MQ=255) gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCa > 1:2994315/1‑70 (MQ=255) gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCa > 1:3476297/1‑70 (MQ=255) aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt < 1:5670630/71‑1 (MQ=255) tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc > 1:389185/1‑71 (MQ=255) aGGTCAGGATATAGAGCACAAAGGCGATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt > 1:2905092/1‑71 (MQ=255) gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt < 1:4258585/70‑1 (MQ=255) cAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCCGCAAATCTTTg > 1:2796383/1‑70 (MQ=255) aGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGt > 1:3723559/1‑71 (MQ=255) ggATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTg < 1:4305190/71‑1 (MQ=255) gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa > 1:5392089/1‑71 (MQ=255) gATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGa > 1:3970433/1‑71 (MQ=255) atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt < 1:2089740/71‑1 (MQ=255) tataGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATg > 1:1344534/1‑71 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTg < 1:841671/66‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTg > 1:1008746/1‑66 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa < 1:3855934/67‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa < 1:5029024/67‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa < 1:5022463/67‑1 (MQ=255) gagCACATAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa < 1:2824126/65‑1 (MQ=255) gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCATATCttt < 1:11791/60‑1 (MQ=255) gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt < 1:4053417/60‑1 (MQ=255) gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt < 1:1146724/60‑1 (MQ=255) gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt < 1:1267016/60‑1 (MQ=255) gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtg > 1:836607/1‑70 (MQ=255) gCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg > 1:4798413/1‑60 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:2050087/71‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:4613688/71‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:3111314/71‑1 (MQ=255) acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa < 1:4131665/67‑1 (MQ=255) acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa < 1:5535236/67‑1 (MQ=255) acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:3226932/70‑1 (MQ=255) cgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaaaa > 1:190574/3‑71 (MQ=255) cgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaaaa > 1:1372428/3‑71 (MQ=255) cgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaaaa < 1:223424/69‑1 (MQ=255) caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATg < 1:3569765/62‑1 (MQ=255) caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATg < 1:307056/62‑1 (MQ=255) caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa < 1:4942605/71‑1 (MQ=255) aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa > 1:2756128/1‑69 (MQ=255) aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt > 1:5260916/1‑48 (MQ=255) aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt > 1:5668755/1‑48 (MQ=255) aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt > 1:3630603/1‑48 (MQ=255) aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc < 1:4633698/49‑1 (MQ=255) aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc < 1:1220998/49‑1 (MQ=255) aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc < 1:721850/49‑1 (MQ=255) aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc > 1:4489128/1‑71 (MQ=255) aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc < 1:2801628/71‑1 (MQ=255) aGGCAATGGAAATGCCGTTAACCACGTTCCAGCCTTTGCCCAGCAAAt > 1:2848161/1‑48 (MQ=255) aGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgtgtta > 1:4137638/1‑63 (MQ=255) aGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTAAAACtg < 1:3774747/71‑2 (MQ=255) ggCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTAAAACtgg < 1:2852568/71‑3 (MQ=255) gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg > 1:2372887/1‑51 (MQ=255) gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa > 1:3868890/1‑55 (MQ=255) aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt < 1:3966389/47‑1 (MQ=255) aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt > 1:5610506/1‑51 (MQ=255) aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt > 1:5439009/1‑51 (MQ=255) aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt > 1:4753208/1‑51 (MQ=255) aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt > 1:940966/1‑51 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaaa > 1:1236361/1‑42 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaaa > 1:5349269/1‑42 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt < 1:4840014/45‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt < 1:2854481/45‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg < 1:1796894/49‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg < 1:1938430/49‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg < 1:1941406/49‑1 (MQ=255) tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt < 1:1311217/45‑1 (MQ=255) tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg > 1:3599880/1‑47 (MQ=255) tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt < 1:1357031/49‑1 (MQ=255) ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc > 1:3105797/1‑37 (MQ=255) ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc > 1:883130/1‑37 (MQ=255) ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCAAGCAAATCttt > 1:1344116/1‑45 (MQ=255) aaaTGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGAtt > 1:2521635/1‑71 (MQ=255) aaaTGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGAtt < 1:4854646/71‑1 (MQ=255) aaTGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCg < 1:2759106/62‑1 (MQ=255) tGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTc < 1:1520045/69‑1 (MQ=255) tGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTc < 1:2190586/69‑1 (MQ=255) gCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTg > 1:4191754/1‑70 (MQ=255) ccGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGt < 1:3230345/70‑1 (MQ=255) ccGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGt > 1:5087710/1‑70 (MQ=255) cGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:268737/51‑1 (MQ=255) gTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa > 1:3957565/1‑52 (MQ=255) gTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc < 1:2726522/60‑1 (MQ=255) gACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTc < 1:126705/70‑1 (MQ=255) cACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGt > 1:869825/1‑71 (MQ=255) cGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTa < 1:3566885/71‑1 (MQ=255) cGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTa < 1:1545702/71‑1 (MQ=255) gCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCa < 1:481581/71‑1 (MQ=255) ccTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTc > 1:3424217/1‑57 (MQ=255) ccTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCa > 1:2062936/1‑58 (MQ=255) ccTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCa > 1:4726282/1‑58 (MQ=255) tttGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTg < 1:2077361/49‑1 (MQ=255) tGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAat < 1:5644314/70‑1 (MQ=255) tGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAat < 1:2934140/70‑1 (MQ=255) cccAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGAtt < 1:3203028/42‑1 (MQ=255) cccAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAatcat < 1:560616/71‑1 (MQ=255) cccAGCAAATCCTTGGTGATGGTGTCAAAACTCGAACCGAtt < 1:5203672/42‑1 (MQ=255) ccAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAatcatc < 1:3729583/71‑1 (MQ=255) ccAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAatcat > 1:3842728/1‑70 (MQ=255) | TATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATC > minE/2130586‑2130725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |