| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,155,529 | A→G | 35.3% | intergenic (+40/+146) | dacB → / ← obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,155,529 | 0 | A | G | 35.3% | 46.4 / 27.9 | 34 | intergenic (+40/+146) | dacB/obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base A (12/10); new base G (2/10); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01 | |||||||||||
TTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTG > minE/2155459‑2155595 | tttGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGatta < 1:2278123/71‑1 (MQ=255) aaaGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACa > 1:2133692/1‑70 (MQ=255) tATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt < 1:2648301/70‑1 (MQ=255) aaaaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcg > 1:2760005/1‑71 (MQ=255) aaaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg > 1:146625/1‑71 (MQ=255) aaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc > 1:2048622/1‑71 (MQ=255) gAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGt > 1:837092/1‑70 (MQ=255) gAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:3823536/71‑1 (MQ=255) ggCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCt > 1:4772423/1‑70 (MQ=255) acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGca > 1:4035108/1‑47 (MQ=255) acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc > 1:4800180/1‑71 (MQ=255) acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc > 1:3364822/1‑71 (MQ=255) caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGtt < 1:3530875/41‑1 (MQ=255) caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGtt < 1:3065056/41‑1 (MQ=255) caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:1301565/59‑1 (MQ=255) tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:2977235/57‑1 (MQ=255) tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:2921788/57‑1 (MQ=255) tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:1927413/57‑1 (MQ=255) gCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc < 1:4255506/70‑1 (MQ=255) cggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc > 1:3673691/2‑70 (MQ=11) ggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa < 1:1553601/71‑1 (MQ=11) ggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa > 1:3050819/1‑70 (MQ=11) ggCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa > 1:5520019/1‑70 (MQ=255) cTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa < 1:3245084/71‑1 (MQ=11) cTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa < 1:3841057/71‑1 (MQ=11) cTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa < 1:3285390/71‑1 (MQ=255) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc < 1:255958/39‑1 (MQ=14) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc < 1:4175532/39‑1 (MQ=14) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc < 1:5139261/39‑1 (MQ=14) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt < 1:3603031/52‑1 (MQ=11) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa < 1:1356754/68‑1 (MQ=11) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa < 1:4236095/68‑1 (MQ=11) acAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc < 1:5145935/39‑1 (MQ=14) cAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCg > 1:4382492/1‑71 (MQ=255) aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa > 1:1573383/1‑46 (MQ=255) attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt < 1:5410303/42‑2 (MQ=255) attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt < 1:864957/42‑2 (MQ=255) attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg > 1:474442/1‑70 (MQ=255) attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCAGCGtt < 1:1713143/42‑2 (MQ=11) | TTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTG > minE/2155459‑2155595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |