| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,155,544 | T→G | 14.3% | intergenic (+55/+131) | dacB → / ← obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,155,544 | 0 | T | G | 14.3% | 99.9 / 17.2 | 58 | intergenic (+55/+131) | dacB/obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base T (24/25); new base G (4/4); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01 | |||||||||||
TATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACAT > minE/2155475‑2155607 | tATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt < 1:2648301/70‑1 (MQ=255) aaaaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcg > 1:2760005/1‑71 (MQ=255) aaaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg > 1:146625/1‑71 (MQ=255) aaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc > 1:2048622/1‑71 (MQ=255) gAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGt > 1:837092/1‑70 (MQ=255) gAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:3823536/71‑1 (MQ=255) ggCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCt > 1:4772423/1‑70 (MQ=255) acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGca > 1:4035108/1‑47 (MQ=255) acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc > 1:4800180/1‑71 (MQ=255) acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc > 1:3364822/1‑71 (MQ=255) caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGtt < 1:3065056/41‑1 (MQ=255) caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGtt < 1:3530875/41‑1 (MQ=255) caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:1301565/59‑1 (MQ=255) tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:1927413/57‑1 (MQ=255) tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:2977235/57‑1 (MQ=255) tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:2921788/57‑1 (MQ=255) gCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc < 1:4255506/70‑1 (MQ=255) cggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc > 1:3673691/2‑70 (MQ=11) ggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa < 1:1553601/71‑1 (MQ=11) ggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa > 1:3050819/1‑70 (MQ=11) ggCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa > 1:5520019/1‑70 (MQ=255) cTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa < 1:3245084/71‑1 (MQ=11) cTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa < 1:3841057/71‑1 (MQ=11) cTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa < 1:3285390/71‑1 (MQ=255) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc < 1:255958/39‑1 (MQ=14) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc < 1:5139261/39‑1 (MQ=14) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc < 1:4175532/39‑1 (MQ=14) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt < 1:3603031/52‑1 (MQ=11) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa < 1:1356754/68‑1 (MQ=11) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa < 1:4236095/68‑1 (MQ=11) acAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc < 1:5145935/39‑1 (MQ=14) cAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCg > 1:4382492/1‑71 (MQ=255) aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa > 1:1573383/1‑46 (MQ=255) attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt < 1:864957/42‑2 (MQ=255) attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt < 1:5410303/42‑2 (MQ=255) attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg > 1:474442/1‑70 (MQ=255) attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCAGCGtt < 1:1713143/42‑2 (MQ=11) tgttGACAACGTACGCATTGTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAACAGTGCa > 1:534654/3‑70 (MQ=11) tgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa > 1:4728969/3‑49 (MQ=11) tgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc > 1:339141/3‑69 (MQ=255) tgctGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCa > 1:4908578/4‑70 (MQ=11) gctGACAAATTGCGCTTTATGT‑TGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAt > 1:2276917/3‑71 (MQ=11) ttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc < 1:2908284/57‑1 (MQ=40) ctGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAAt > 1:4521451/2‑71 (MQ=255) tGACAAATTGCGCTTTATGT‑TGCCGGATGCGGCGTAAACGCCTTATcc > 1:4614147/1‑48 (MQ=11) tGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTa < 1:3205179/46‑1 (MQ=40) aaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt > 1:297689/3‑71 (MQ=11) gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt > 1:1553547/1‑70 (MQ=255) aCAAATTGCGCTTTATGT‑TGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa > 1:4820455/1‑71 (MQ=11) aCAAATTGCGCTTTATGT‑TGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa > 1:2350845/1‑71 (MQ=11) aCAAATTGCGCTTTATGT‑TGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa > 1:4762957/1‑71 (MQ=11) aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc > 1:1072952/1‑70 (MQ=39) aacaGTGCGCTTTGTTTATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCaa > 1:4747307/4‑71 (MQ=11) aaaGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCaat < 1:422614/71‑2 (MQ=255) aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaatac < 1:4900152/71‑4 (MQ=9) tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa > 1:2710053/1‑66 (MQ=255) tGCGCTTTGTTTATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCaata < 1:4563233/68‑3 (MQ=11) tGCGCTTTGTTTATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCaata < 1:4553164/68‑3 (MQ=11) cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa < 1:970542/55‑1 (MQ=255) | TATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACAT > minE/2155475‑2155607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |