| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,155,605 | C→T | 41.7% | intergenic (+116/+70) | dacB → / ← obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,155,605 | 0 | C | T | 41.7% | 105.3 / 77.3 | 72 | intergenic (+116/+70) | dacB/obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base C (31/11); new base T (16/14); total (47/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.44e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01 | |||||||||||
TTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAG > minE/2155545‑2155671 | ttGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAAt > 1:1546328/1‑71 (MQ=255) tGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:5692738/1‑64 (MQ=40) tGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:276101/1‑64 (MQ=40) tGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:4561491/71‑1 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc > 1:444215/1‑56 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCa > 1:3612334/1‑57 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc‑t < 1:891784/60‑1 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCt < 1:5120457/71‑1 (MQ=255) cggcgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCt > 1:253713/1‑70 (MQ=11) cggcgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg < 1:2459078/71‑1 (MQ=14) cgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:695466/59‑1 (MQ=255) cgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGa > 1:2469852/1‑68 (MQ=255) cgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGAt > 1:2812655/1‑69 (MQ=255) cgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa < 1:1772080/71‑1 (MQ=14) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc < 1:5697768/47‑1 (MQ=255) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:1200361/57‑1 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTa > 1:3049852/1‑57 (MQ=255) aaCGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCg > 1:4316326/1‑71 (MQ=11) aCGCCTTATCCGGCCTACAAAATCGTGCTAATTCAACATATTGCAATTC‑TCTTg < 1:3097118/54‑1 (MQ=255) aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:5603832/54‑1 (MQ=255) aCGCCTTATCCGGCCTACAAAATCCTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:3922463/54‑1 (MQ=255) cGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCgca < 1:775285/71‑3 (MQ=11) cTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAgcg > 1:1525361/1‑70 (MQ=255) aTCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc‑t > 1:4672749/1‑43 (MQ=255) aTCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:2995949/1‑70 (MQ=255) aTCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAt > 1:2438123/1‑71 (MQ=11) tCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc‑t > 1:1891833/1‑42 (MQ=255) tCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATc < 1:218615/71‑1 (MQ=11) ccGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGt > 1:4635591/1‑46 (MQ=255) gCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGc < 1:2681351/71‑1 (MQ=11) gCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGc < 1:4763606/71‑1 (MQ=11) cTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAg > 1:5235795/1‑58 (MQ=255) cTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAg > 1:5233610/1‑58 (MQ=255) tACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGcaat > 1:5363373/1‑68 (MQ=0) aCAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAtt > 1:381283/1‑71 (MQ=255) cataaTCTTGAAAATTCAATATATTGCAGTTA‑TCTGGTAGGCCAGGTAAGCGTAGCGCATc > 1:3137942/4‑61 (MQ=2) cataaTCTTGAAAATTCAATATATTGCAGTTA‑TCTGGTAGGCCAGGTAAGCGTAGCGCATc > 1:4678403/4‑61 (MQ=2) cataaTCTTGAAAATTCAATATATTGCAGTTA‑TCTGGTAGGCCAGGTAAGCGTAGCGCATc > 1:2272322/4‑61 (MQ=2) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt > 1:623795/1‑71 (MQ=255) cAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGcaat < 1:1641788/69‑4 (MQ=0) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt > 1:1547663/1‑70 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt > 1:1090193/1‑70 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTg > 1:3142566/1‑71 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTg > 1:3810780/1‑71 (MQ=255) aaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGt > 1:3145127/1‑35 (MQ=255) aaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:2970338/1‑57 (MQ=255) aaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGc > 1:4833084/1‑71 (MQ=255) aatgTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC‑AATTTGGCg < 1:4549032/68‑1 (MQ=2) aTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGc > 1:724251/1‑38 (MQ=255) tCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAtt < 1:1816328/65‑1 (MQ=255) cgCAAATTCAATAGATTGCAATGC‑ACTTGTAGGCCTGATAAGc > 1:4031576/2‑43 (MQ=11) cgCAAATTCAATAGATTGCAATGC‑ACTTGTAGGCCTGATAAGCg > 1:4014754/2‑44 (MQ=11) cgCAAATTCAATAGATTGCAATGC‑ACTTGTAGGCCTGATAAGCg > 1:3318039/2‑44 (MQ=11) cAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGAt > 1:1325812/1‑38 (MQ=9) cAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATaa > 1:4732087/1‑40 (MQ=9) cAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCa < 1:830321/53‑1 (MQ=11) cAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC‑AATTTGGCGttgcc < 1:1749085/70‑4 (MQ=2) cAAATTAAATAAATTGCAATTCAACTTGTAGGCCTGATaa > 1:4538888/1‑40 (MQ=9) aaaTTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC‑AATTTGGCGtt < 1:163315/66‑1 (MQ=2) aaaTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatca > 1:4118040/1‑71 (MQ=255) aaaTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatca > 1:3386257/1‑71 (MQ=255) aaTTCAATAAATTGCAATTCATCTTGTAGGCCTGATAAGCGCAGCGCATCAGGC‑AATTTGGCGtt < 1:2665475/65‑1 (MQ=11) aTTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGc < 1:495751/39‑1 (MQ=2) aTTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGc < 1:5051387/39‑1 (MQ=2) aTTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGcaa > 1:3344038/1‑53 (MQ=0) aTTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC‑AATTTGGCGttgc > 1:1678013/1‑64 (MQ=2) aTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatcat > 1:4919494/1‑70 (MQ=255) aTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatcat > 1:5502185/1‑70 (MQ=255) ttCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCg > 1:3941578/1‑39 (MQ=2) ttCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCt < 1:1600126/52‑1 (MQ=255) tCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAg > 1:5525137/1‑71 (MQ=255) tCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAg > 1:5107934/1‑71 (MQ=255) cATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTa < 1:5125964/60‑1 (MQ=255) cATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTa < 1:1990295/60‑1 (MQ=255) | TTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAG > minE/2155545‑2155671 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |