| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,220,168 | C→A | 11.8% | intergenic (+428/‑550) | yhdH → / → accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,220,168 | 0 | C | A | 11.8% | 280.7 / 14.8 | 93 | intergenic (+428/‑550) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base C (39/43); new base A (7/4); total (46/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01 | |||||||||||
TTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATC > minE/2220104‑2220238 | ttGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGc > 1:3907912/1‑70 (MQ=255)ttGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCg > 1:5294878/1‑71 (MQ=255)ttGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCg > 1:48508/1‑71 (MQ=255) gCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGt < 1:3470197/70‑1 (MQ=255) gCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGt < 1:1463045/70‑1 (MQ=255) gTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGt < 1:1023665/68‑1 (MQ=255) tGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTa < 1:1816896/68‑1 (MQ=255) tGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATg < 1:918950/71‑1 (MQ=255) gCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATgt > 1:1633661/1‑71 (MQ=255) gCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATgt > 1:481108/1‑71 (MQ=255) cTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATgtc > 1:4470631/1‑71 (MQ=255) tAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATgtcgt < 1:3038778/70‑1 (MQ=255) aTTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATc > 1:2328295/1‑71 (MQ=255) acaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTa > 1:1958122/1‑55 (MQ=255) acaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTa > 1:502112/1‑55 (MQ=255) tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGt < 1:5001991/51‑1 (MQ=255) tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCt > 1:725991/1‑65 (MQ=255) cTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgc > 1:3169424/1‑70 (MQ=255) aacccAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGa < 1:4306231/68‑1 (MQ=255) aacccAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGa < 1:4653670/68‑1 (MQ=255) aTCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGa > 1:468223/1‑70 (MQ=255) tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgc < 1:1828754/67‑1 (MQ=255) tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGa < 1:5020788/69‑1 (MQ=255) tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:4505061/71‑1 (MQ=255) cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc < 1:4283239/70‑1 (MQ=255) aggcGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTg > 1:2603526/4‑71 (MQ=255) aggcGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTg < 1:3139613/68‑1 (MQ=255) gATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCAt > 1:2346852/1‑71 (MQ=255) gATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGa > 1:1835493/1‑62 (MQ=255) gATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGa > 1:1681589/1‑62 (MQ=255) gATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGa > 1:3365152/1‑62 (MQ=255) gCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc > 1:5396563/1‑71 (MQ=255) aGTGAAAATTGGGTAATCCCCAGCTACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTa < 1:475777/71‑1 (MQ=255) aGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTaa > 1:439939/1‑37 (MQ=255) aGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTaa > 1:5144609/1‑37 (MQ=255) aGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTaa > 1:3432889/1‑37 (MQ=255) aGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTc > 1:4740542/1‑53 (MQ=255) gTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgcg < 1:2699941/55‑1 (MQ=255) gTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCAt < 1:3595115/65‑1 (MQ=255) gTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCAt < 1:1836574/65‑1 (MQ=255) tGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc < 1:4539228/58‑1 (MQ=255) tGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg < 1:3516440/60‑1 (MQ=255) tGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg < 1:4295675/60‑1 (MQ=255) tGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg < 1:1083546/60‑1 (MQ=255) gAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCtt > 1:1414804/1‑47 (MQ=255) gAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc > 1:2118056/1‑56 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATgtcg > 1:2629802/1‑38 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTAtaa > 1:1496903/1‑43 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTc > 1:3933827/1‑49 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg > 1:925135/1‑58 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg > 1:5627965/1‑58 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACat > 1:267293/1‑70 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGAACCTGGCATCCCTACat > 1:4004785/1‑70 (MQ=255) aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:3311709/54‑1 (MQ=255) aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:577148/54‑1 (MQ=255) aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:3975953/54‑1 (MQ=255) aaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattat > 1:3353499/1‑71 (MQ=255) aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt < 1:3386335/71‑1 (MQ=255) aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt < 1:5607574/71‑1 (MQ=255) ttGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt > 1:2948697/1‑70 (MQ=255) ttGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt > 1:2122867/1‑70 (MQ=255) ttGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTAttt < 1:2925540/71‑1 (MQ=255) ttGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTAttt < 1:162078/71‑1 (MQ=255) tGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCtt < 1:4014550/41‑1 (MQ=255) tGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCtt < 1:5149069/41‑1 (MQ=255) tAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:2871434/46‑1 (MQ=255) tAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:1427390/46‑1 (MQ=255) aaTCCCCAGCAACCGCTGCGTAATGTCGTGGATCTTGTCGCGTTCCTGGCATCCCTACATTTTTTGTgg > 1:4540535/1‑69 (MQ=255) aaTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTAc < 1:3924974/58‑1 (MQ=255) aTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCt > 1:5601656/1‑46 (MQ=255) aTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTc < 1:1818535/70‑1 (MQ=255) ccccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgt > 1:627322/1‑71 (MQ=255) cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg < 1:2005162/71‑1 (MQ=255) cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg < 1:1736173/71‑1 (MQ=255) ctggCAACAGTTGCATAATTTCGTCTATCTTGTGGCGATTCTGGCCCCAATACATTATCTGTGGGCCGTGa < 1:4910396/68‑1 (MQ=255) ctggCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgtg > 1:4042929/4‑70 (MQ=255) ccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCt < 1:1148480/42‑1 (MQ=255) cAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg > 1:3741447/1‑69 (MQ=255) ggCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgtg > 1:3262699/2‑68 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc > 1:1471143/1‑38 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc > 1:2532016/1‑38 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc < 1:2398680/47‑1 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc < 1:3870149/47‑1 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc < 1:4257752/47‑1 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc < 1:4311493/47‑1 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGt > 1:673363/1‑62 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGt > 1:5648965/1‑62 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGt > 1:3018610/1‑62 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGa < 1:1257394/71‑1 (MQ=255) gCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgtg > 1:1397027/1‑67 (MQ=255) aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatta < 1:204342/52‑1 (MQ=255) aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatta < 1:2460804/52‑1 (MQ=255) aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt < 1:5545810/71‑1 (MQ=255) cGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGc > 1:4422049/1‑37 (MQ=255) cGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtc > 1:2923897/1‑71 (MQ=255) cGCTGCGTAATGTCGTCTAACTTGTCGCGATCCTGGc > 1:5650794/1‑37 (MQ=255) | TTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATC > minE/2220104‑2220238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |