| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,220,199 | C→T | 29.9% | intergenic (+459/‑519) | yhdH → / → accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,220,199 | 0 | C | T | 29.9% | 144.5 / 74.6 | 107 | intergenic (+459/‑519) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base C (33/42); new base T (13/19); total (46/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
TCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCT > minE/2220129‑2220267 | tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:4505061/71‑1 (MQ=255) cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc < 1:4283239/70‑1 (MQ=255) aggcGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTg > 1:2603526/4‑71 (MQ=255) aggcGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTg < 1:3139613/68‑1 (MQ=255) gATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCAt > 1:2346852/1‑71 (MQ=255) gCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc > 1:5396563/1‑71 (MQ=255) aGTGAAAATTGGGTAATCCCCAGCTACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTa < 1:475777/71‑1 (MQ=255) gTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCAt < 1:1836574/65‑1 (MQ=255) tGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc < 1:4539228/58‑1 (MQ=255) tGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg < 1:1083546/60‑1 (MQ=255) tGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg < 1:4295675/60‑1 (MQ=255) tGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg < 1:3516440/60‑1 (MQ=255) gAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc > 1:2118056/1‑56 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg > 1:5627965/1‑58 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGAACCTGGCATCCCTACat > 1:4004785/1‑70 (MQ=255) aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:3975953/54‑1 (MQ=255) aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:577148/54‑1 (MQ=255) aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:3311709/54‑1 (MQ=255) aaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattat > 1:3353499/1‑71 (MQ=255) aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt < 1:3386335/71‑1 (MQ=255) ttGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt > 1:2948697/1‑70 (MQ=255) ttGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTAttt < 1:2925540/71‑1 (MQ=255) ttGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTAttt < 1:162078/71‑1 (MQ=255) tAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:2871434/46‑1 (MQ=255) tAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:1427390/46‑1 (MQ=255) aTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCt > 1:5601656/1‑46 (MQ=255) aTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTc < 1:1818535/70‑1 (MQ=255) cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg < 1:1736173/71‑1 (MQ=255) ctggCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgtg > 1:4042929/4‑70 (MQ=255) ccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCt < 1:1148480/42‑1 (MQ=255) cAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg > 1:3741447/1‑69 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc > 1:2532016/1‑38 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc > 1:1471143/1‑38 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc < 1:3870149/47‑1 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc < 1:2398680/47‑1 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc < 1:4257752/47‑1 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGt > 1:5648965/1‑62 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGt > 1:3018610/1‑62 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGt > 1:673363/1‑62 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGa < 1:1257394/71‑1 (MQ=255) gCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgtg > 1:1397027/1‑67 (MQ=255) aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatta < 1:2460804/52‑1 (MQ=255) aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatta < 1:204342/52‑1 (MQ=255) aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt < 1:5545810/71‑1 (MQ=255) cGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGc > 1:4422049/1‑37 (MQ=255) cGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtc > 1:2923897/1‑71 (MQ=255) cGCTGCGTAATGTCGTCTAACTTGTCGCGATCCTGGc > 1:5650794/1‑37 (MQ=255) gCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtct > 1:4713829/1‑71 (MQ=255) ttGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACatta < 1:5260081/46‑1 (MQ=255) ttGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACatta < 1:2462908/46‑1 (MQ=255) ttGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACatta < 1:4317539/46‑1 (MQ=255) ttGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTAtttc < 1:2915009/70‑1 (MQ=255) ttGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTAtttc < 1:431565/70‑1 (MQ=255) cTGCGTAATGTCGTCTATCTTGTCGCGCTCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtct > 1:4510203/1‑70 (MQ=255) tGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg < 1:898184/59‑1 (MQ=255) tGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCa > 1:5541425/1‑71 (MQ=255) cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa < 1:1744959/69‑1 (MQ=255) cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa < 1:1943736/69‑1 (MQ=255) cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAt > 1:5020110/1‑70 (MQ=255) gTAATGTCGTCTCTCTTGTCGCGATCCTGGCATCCCTACATTATTTGTTGTCTGTGAAGATTATCTCATTg < 1:1870458/71‑1 (MQ=255) gTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtgtg > 1:310254/1‑49 (MQ=255) gTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTg < 1:3085671/71‑1 (MQ=255) aaTGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTg > 1:917296/1‑69 (MQ=255) tttcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGaa < 1:1426304/52‑1 (MQ=255) tttcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGaa < 1:1332719/52‑1 (MQ=255) tttcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGa > 1:1970645/3‑56 (MQ=255) tttcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGa > 1:396614/3‑56 (MQ=255) tttcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGa > 1:3718100/3‑56 (MQ=255) tcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGa > 1:1127986/1‑54 (MQ=255) tcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGccg > 1:2110651/1‑70 (MQ=255) tcgtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGccc > 1:3997921/1‑71 (MQ=255) gtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAg > 1:428509/1‑51 (MQ=255) gtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGa > 1:1761596/1‑52 (MQ=255) tcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTc < 1:4592050/71‑1 (MQ=255) tATCTTGTCGCGATTCTGGCCCCAATACATTATCtg < 1:1120337/36‑1 (MQ=255) tATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtgt < 1:2807697/55‑3 (MQ=255) tATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtct < 1:1077216/55‑1 (MQ=255) tATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCCCAt > 1:5414755/1‑71 (MQ=255) aTCTTGTGGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTAttt < 1:3900014/54‑3 (MQ=255) tCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGaa > 1:3616423/1‑45 (MQ=255) tCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCa < 1:1933036/55‑1 (MQ=255) tCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATc < 1:3981274/70‑1 (MQ=255) tCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa < 1:5494708/44‑1 (MQ=255) tCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa > 1:675890/1‑55 (MQ=255) tCTTGTCGCGATCCGGGCATCCCTACATTATTTGTGGTCTGTGa < 1:5676670/44‑1 (MQ=255) cTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGAt < 1:5350850/47‑1 (MQ=255) cTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGAt < 1:1378320/47‑1 (MQ=255) ttGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGAt < 1:3489104/46‑1 (MQ=255) ttGTCGCGATCCTGGCATCCCTACATTATTTGTGGt > 1:303364/1‑36 (MQ=255) gTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCt < 1:465123/67‑1 (MQ=255) gTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGc > 1:1666235/1‑71 (MQ=255) gTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCtt < 1:483433/68‑1 (MQ=255) gTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCg < 1:876015/70‑1 (MQ=255) tCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGct > 1:610291/1‑70 (MQ=255) tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGc < 1:5574589/70‑1 (MQ=255) gcgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAg < 1:2060615/70‑1 (MQ=255) cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTc < 1:2089726/59‑1 (MQ=255) cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTc < 1:3710448/59‑1 (MQ=255) cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTc < 1:3938216/59‑1 (MQ=255) aTCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct < 1:5189383/71‑1 (MQ=255) | TCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCT > minE/2220129‑2220267 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |