| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,294,089 | C→A | 43.6% | intergenic (+116/‑159) | gldA → / → yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,294,089 | 0 | C | A | 43.6% | 74.1 / 70.5 | 71 | intergenic (+116/‑159) | gldA/yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (19/21); new base A (18/13); total (37/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01 | |||||||||||
TGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAATT > minE/2294022‑2294159 | tGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGa > 1:1324775/1‑71 (MQ=255)tGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGa > 1:2831294/1‑71 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGa > 1:4901081/1‑70 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt < 1:4455300/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATa < 1:973445/71‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt > 1:1621580/1‑70 (MQ=255) tGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATaa < 1:4767790/71‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAg < 1:2057525/70‑1 (MQ=255) cTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCg < 1:1017138/71‑1 (MQ=255) cTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCg < 1:581835/71‑1 (MQ=255) cGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:4114046/70‑1 (MQ=255) cTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCAc > 1:3613057/1‑71 (MQ=255) tATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCg < 1:54883/64‑1 (MQ=255) tATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCg < 1:2644355/64‑1 (MQ=255) aTCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTcc > 1:1376865/1‑71 (MQ=255) tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt < 1:2993334/56‑1 (MQ=17) tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGtt < 1:1285377/64‑1 (MQ=255) cAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCg > 1:4720792/1‑70 (MQ=255) aGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:1977081/1‑56 (MQ=17) aGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:592788/1‑56 (MQ=17) aGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCa < 1:3097563/71‑1 (MQ=255) gCCTACGCCATCTCTGCAATATATTGAATTTGCTTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATc < 1:5565589/71‑1 (MQ=14) gCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATc < 1:350415/71‑1 (MQ=14) cTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:4650485/1‑52 (MQ=14) cTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCg > 1:3390699/1‑71 (MQ=11) tACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:2706362/57‑1 (MQ=255) tACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:5361768/57‑1 (MQ=255) aCGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:776566/48‑1 (MQ=255) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:827485/1‑49 (MQ=14) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:4195964/1‑49 (MQ=14) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:2887121/1‑49 (MQ=14) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTc > 1:4768019/1‑56 (MQ=14) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCgg < 1:1258126/69‑1 (MQ=11) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCgg < 1:2120277/69‑1 (MQ=11) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCa > 1:3472742/1‑71 (MQ=11) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCa > 1:2113870/1‑71 (MQ=11) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCa > 1:4381388/1‑71 (MQ=11) cGCAATCTCTGCAATATAATGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCTTCCgg < 1:4109549/69‑1 (MQ=255) tctcTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt < 1:1518826/42‑1 (MQ=255) tctcTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATa < 1:4591468/43‑1 (MQ=255) ctctGTAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCg > 1:749650/1‑47 (MQ=255) ctctGCAATATATTGAATTTGCGTGCTTTTGTAAGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCtgt > 1:2420119/1‑71 (MQ=255) tctGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCa > 1:4102370/1‑64 (MQ=40) tctGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:4010011/1‑42 (MQ=255) ctGCAATATATTGAATTTGCGTGCTTTTTTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGc > 1:5678167/1‑71 (MQ=255) ctGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:5568961/47‑1 (MQ=38) ctGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:3646079/47‑1 (MQ=38) tGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGc > 1:4100520/1‑70 (MQ=255) cAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCa < 1:2137637/46‑1 (MQ=38) cAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCa < 1:5045231/46‑1 (MQ=38) cAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt < 1:50652/36‑1 (MQ=255) aaTATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCActct > 1:3623469/1‑63 (MQ=255) atatatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGt > 1:1044988/1‑70 (MQ=255) atatatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGt < 1:1771631/70‑1 (MQ=255) atatatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTa < 1:3342011/71‑1 (MQ=255) atatatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTa < 1:2666049/71‑1 (MQ=255) tatTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCa > 1:4161596/1‑41 (MQ=255) tatTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCa > 1:1820872/1‑41 (MQ=255) atTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCa > 1:704235/1‑40 (MQ=40) ttGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCt > 1:540453/1‑70 (MQ=255) tGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTc > 1:5021569/1‑37 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCTGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGa < 1:770221/70‑1 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGa < 1:2242911/70‑1 (MQ=255) aTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCt > 1:3459319/1‑66 (MQ=255) aTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCt > 1:3457347/1‑66 (MQ=255) tttGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTc < 1:150415/37‑1 (MQ=37) ttGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAAt > 1:2507610/1‑62 (MQ=255) ttGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAAt > 1:815692/1‑62 (MQ=255) tGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATcc > 1:3887064/1‑42 (MQ=38) tGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACgttgtt > 1:543986/1‑70 (MQ=255) gTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCActc < 1:1144228/38‑1 (MQ=40) ccGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTc > 1:1958157/1‑65 (MQ=255) cGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAAtt > 1:5140115/1‑71 (MQ=255) | TGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAATT > minE/2294022‑2294159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |