| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,303,856 | C→T | 17.0% | intergenic (+29/‑155) | metJ → / → yiiX | DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,303,856 | 0 | C | T | 17.0% | 101.9 / 13.1 | 47 | intergenic (+29/‑155) | metJ/yiiX | DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase |
| Reads supporting (aligned to +/- strand): ref base C (14/25); new base T (3/5); total (17/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01 | |||||||||||
GTGAGATGGGGATTAACCCGGAGACGTGGGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTA > minE/2303790‑2303922 | gTGAGATGGGGATTAACCCGGAGACGTGGGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCt < 1:1562448/71‑1 (MQ=255) tGAGATGGGGATTAACCCGGAGACGTGGGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCt > 1:2144752/1‑70 (MQ=255) ggggATTAACCCGGAGACGTGGGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGATTCGCCTGATgcg > 1:3877934/1‑70 (MQ=255) tAACCCGGAGACGTGGGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCT > 1:1229701‑M1/1‑71 (MQ=255) tAACCCGGAGACGTGGGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCT > 1:2036374‑M1/1‑71 (MQ=255) aaCCCGGAGACGTGGGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCT > 1:5656704‑M1/1‑70 (MQ=255) aCCCGGAGACGTGGGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTA > 1:3037683‑M1/1‑71 (MQ=255) aCCCGGAGACGTGGGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTT < 1:4297467‑M1/70‑1 (MQ=255) cGGAGACGTGGGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCA < 1:3562871‑M1/71‑1 (MQ=255) gaCGTGGGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCt < 1:2344273/57‑1 (MQ=255) ggAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGt > 1:2796021/1‑70 (MQ=255) gAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCa < 1:308941/71‑1 (MQ=255) tACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTA > 1:5352514‑M1/1‑63 (MQ=255) tACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTT < 1:207959‑M1/53‑1 (MQ=255) tAAAGCAAAAAAGACCGGCGCTGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCat < 1:787971/66‑1 (MQ=255) aaGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCa < 1:2753124/70‑1 (MQ=255) gCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAAtt > 1:2785279/1‑71 (MQ=255) aaaaaaGAGCGGCGGGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTa < 1:1439509/71‑1 (MQ=255) aaaaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTA > 1:1297954‑M1/1‑53 (MQ=255) aaaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTAtt > 1:560992/1‑71 (MQ=255) aaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTAtt > 1:446652/1‑70 (MQ=255) aaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg < 1:456896/71‑1 (MQ=255) agagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg < 1:3538734/69‑1 (MQ=255) agagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg < 1:2476149/69‑1 (MQ=255) gagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAAt < 1:3732742/71‑1 (MQ=255) cgGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTa < 1:3937039/57‑1 (MQ=255) gctggAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcg < 1:423301‑M1/69‑33 (MQ=255) gTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCa < 1:3706707/41‑1 (MQ=255) gTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACtt < 1:4268655/71‑1 (MQ=255) tGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCat < 1:182463/41‑1 (MQ=255) tGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAAATTATTGAATTTGCACGAACTTg < 1:197399/71‑1 (MQ=255) ggAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgtt > 1:1703141‑M1/1‑36 (MQ=255) ggAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgtt > 1:4305531‑M1/1‑36 (MQ=255) ggAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggc < 1:2998829‑M1/67‑32 (MQ=255) gAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttca > 1:2826639‑M1/1‑35 (MQ=255) gAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgtt < 1:1040741‑M1/69‑35 (MQ=255) gAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgtt < 1:2241225‑M1/69‑35 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTg < 1:1978944/59‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt < 1:4594440/70‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt < 1:1434517/70‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt < 1:843407/70‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGACCTACGTCATATTGCAATTTa > 1:674553/1‑50 (MQ=255) aaTTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgtt < 1:4719136‑M1/68‑35 (MQ=255) aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAAt < 1:4816554/55‑1 (MQ=255) aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTg < 1:4278153/68‑1 (MQ=255) tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGa < 1:1657780/51‑1 (MQ=255) tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTACTGAATTTg > 1:327091/1‑56 (MQ=255) | GTGAGATGGGGATTAACCCGGAGACGTGGGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTA > minE/2303790‑2303922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |