| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,303,906 | A→T | 11.4% | intergenic (+79/‑105) | metJ → / → yiiX | DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,303,906 | 0 | A | T | 11.4% | 216.9 / 19.8 | 88 | intergenic (+79/‑105) | metJ/yiiX | DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase |
| Reads supporting (aligned to +/- strand): ref base A (42/36); new base T (6/4); total (48/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACGTTGTC > minE/2303837‑2303974 | gagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAAt < 1:3732742/71‑1 (MQ=255) gTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACtt < 1:4268655/71‑1 (MQ=255) tGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAAATTATTGAATTTGCACGAACTTg < 1:197399/71‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTg < 1:1978944/59‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGt < 1:843407/70‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGt < 1:4594440/70‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGt < 1:1434517/70‑1 (MQ=255) aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAAt < 1:4816554/55‑1 (MQ=255) aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTg < 1:4278153/68‑1 (MQ=255) tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTACTGAATTTg > 1:327091/1‑56 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGc > 1:4704022/1‑69 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt < 1:157414/69‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt < 1:5246844/69‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt > 1:3972239/1‑69 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt < 1:2440714/69‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGa > 1:3907292/1‑70 (MQ=2) tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt > 1:3459869/1‑70 (MQ=2) tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt > 1:3803956/1‑70 (MQ=2) gcTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCg > 1:3102765/1‑71 (MQ=255) cTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGc < 1:3405734/59‑1 (MQ=255) tACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt > 1:4047786/1‑71 (MQ=255) tACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt > 1:5184782/1‑71 (MQ=255) aCGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt > 1:3891404/1‑70 (MQ=255) cGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt < 1:3029182/71‑1 (MQ=2) gCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTa > 1:3582468/1‑52 (MQ=255) cTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATa > 1:5691889/1‑60 (MQ=255) ttATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt < 1:303607/68‑1 (MQ=2) aTCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCAcgccg > 1:3580256/1‑71 (MQ=255) aTCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCAcgc > 1:3866421/1‑71 (MQ=2) aGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAgg > 1:4349288/1‑47 (MQ=255) aGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt < 1:3365622/53‑1 (MQ=255) aGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:5221789/71‑1 (MQ=255) ggCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:3792219/71‑1 (MQ=255) gCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATcc > 1:3514639/1‑71 (MQ=255) gCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:2469343/70‑1 (MQ=255) cTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt > 1:426077/1‑56 (MQ=255) cTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:5416851/71‑1 (MQ=255) aCGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGa < 1:2987731/46‑1 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa > 1:3843765/1‑48 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa > 1:2546639/1‑48 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa > 1:3740576/1‑70 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa > 1:4573581/1‑70 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa > 1:3871214/1‑70 (MQ=255) gTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa > 1:3675332/1‑55 (MQ=255) tCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTa > 1:2644667/1‑36 (MQ=255) tCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTa > 1:188142/1‑36 (MQ=255) atat‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaac < 1:4141506/71‑1 (MQ=255) tat‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaaca < 1:856903/71‑1 (MQ=255) at‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaa > 1:2532159/1‑71 (MQ=255) at‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaa > 1:2453031/1‑71 (MQ=255) tggCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAaaaaa > 1:4308557/3‑67 (MQ=14) t‑GCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAaacaac > 1:948933/1‑70 (MQ=14) t‑GCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAaacaac > 1:3159430/1‑70 (MQ=14) t‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa > 1:1396753/1‑40 (MQ=255) t‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa > 1:1536045/1‑40 (MQ=255) t‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAgg > 1:5499850/1‑42 (MQ=255) t‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAgg > 1:4843121/1‑42 (MQ=255) cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa < 1:2203729/39‑1 (MQ=9) cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa < 1:1560332/39‑1 (MQ=9) cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa > 1:252804/2‑40 (MQ=9) cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa < 1:2282605/39‑1 (MQ=9) cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa < 1:4310830/39‑1 (MQ=9) cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATa > 1:4462287/2‑39 (MQ=9) gCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaac > 1:127821/1‑69 (MQ=255) gCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGAt > 1:4527265/1‑37 (MQ=9) aTTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaa > 1:4600911/1‑65 (MQ=255) tttATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGcc > 1:3177189/1‑69 (MQ=11) tttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt < 1:3885705/40‑1 (MQ=255) tttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt < 1:2223893/40‑1 (MQ=255) tttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt < 1:5144503/40‑1 (MQ=255) tttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt < 1:3047079/40‑1 (MQ=255) ttattaAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCACGCCGCa < 1:1191464/49‑1 (MQ=14) ttattaAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCACGCCGCa < 1:3061514/49‑1 (MQ=14) ttattaAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCACGCCGCa < 1:3138717/49‑1 (MQ=14) ttattaAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCACGCCGCa < 1:1336786/49‑1 (MQ=14) ttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACg > 1:5221284/1‑71 (MQ=255) tATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa > 1:2428901/1‑41 (MQ=38) tATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa > 1:2501847/1‑41 (MQ=38) tATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa > 1:1490483/1‑41 (MQ=38) tATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATaaa > 1:4563262/1‑59 (MQ=255) tATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACGt > 1:2382162/1‑71 (MQ=255) aTTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCa > 1:1024020/1‑40 (MQ=14) aTTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCa > 1:5552732/1‑40 (MQ=14) ttGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACGtt < 1:1826962/70‑1 (MQ=255) ttGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACGtt < 1:3852601/70‑1 (MQ=255) gAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt < 1:4438711/35‑1 (MQ=40) gAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt < 1:85072/35‑1 (MQ=40) gAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt < 1:1782587/35‑1 (MQ=40) aTTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACGTTGTc > 1:4089437/1‑69 (MQ=255) | GAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACGTTGTC > minE/2303837‑2303974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |