| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 106,094 | C→A | 12.1% | L66L (CTG→CTT) | aroP ← | aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 106,094 | 0 | C | A | 12.1% | 118.8 / 4.1 | 41 | L66L (CTG→CTT) | aroP | aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base C (17/19); new base A (2/3); total (19/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01 | |||||||||||
CTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCG > minE/106026‑106163 | cTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAg < 1:5270303/71‑1 (MQ=255) tccccAGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCt < 1:1085254/70‑1 (MQ=255) tccccAGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCt < 1:5349701/70‑1 (MQ=255) gCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCt > 1:2700314/1‑71 (MQ=255) aGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCTGGCg > 1:1605763/1‑70 (MQ=255) aGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGc < 1:606703/71‑1 (MQ=255) aTTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCTGGCGCAt > 1:1419409/1‑70 (MQ=255) tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTg < 1:856727/63‑1 (MQ=255) tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATg < 1:5494395/70‑1 (MQ=255) aaaGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCt > 1:3789774/1‑53 (MQ=255) aaaGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAgg > 1:4151564/1‑71 (MQ=255) gTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTTACCCa < 1:2182475/47‑1 (MQ=255) tGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGAt < 1:178533/71‑1 (MQ=255) tGGCTAAAGGAGCATGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGAt < 1:482131/71‑1 (MQ=255) aaGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAc > 1:3476152/1‑70 (MQ=255) aGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCa < 1:2706303/71‑1 (MQ=255) gAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc < 1:3640472/71‑1 (MQ=255) gCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCaa > 1:3768620/1‑71 (MQ=255) cTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATg < 1:452202/71‑1 (MQ=255) cTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATg < 1:815917/71‑1 (MQ=255) cTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATg < 1:2768478/71‑1 (MQ=255) cTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATg < 1:168769/71‑1 (MQ=255) tGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc < 1:2659560/66‑1 (MQ=255) tGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATgg > 1:924761/1‑71 (MQ=255) ggTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAAcc > 1:4568779/1‑71 (MQ=255) tcttcGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAcc > 1:3464436/1‑53 (MQ=255) tcttcGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAc > 1:2033309/1‑52 (MQ=255) tcttcGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAg > 1:705734/1‑71 (MQ=255) tcttcGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAg > 1:846298/1‑71 (MQ=255) cttcGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAg < 1:4017139/70‑1 (MQ=255) ttcGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGa > 1:3414702/1‑71 (MQ=255) aaccaccATTTCGCCAAGCTGGCGCATAATCAGGAAAGc < 1:3470610/38‑1 (MQ=12) caccaTTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGt < 1:1100088/56‑1 (MQ=255) tttCACCCAGCTGACGCATGATCAGAAAGGCGATaa < 1:1120233/36‑1 (MQ=255) ttCACCCAGCTGACGCATGATCAGAAAGGCGATaaaa > 1:4942523/1‑37 (MQ=255) ttCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAc > 1:89526/1‑38 (MQ=255) ttCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAc > 1:4069261/1‑38 (MQ=255) tCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGTATAATCCCTg > 1:3089723/1‑67 (MQ=255) tCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATcc > 1:2811649/1‑64 (MQ=255) aCCCATCTGACGCATGATCAGAAAGGCGATAAAAcc < 1:953404/36‑1 (MQ=255) aCCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAAt < 1:2547607/60‑1 (MQ=255) ccAGCTGACGCATGATCAGAAAGGCGATAAAACCAg > 1:2647180/1‑36 (MQ=255) ccAGCTGACGCATGATCAGAAAGGCGATAAAACCAg > 1:1595452/1‑36 (MQ=255) ccAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAAt > 1:3754345/1‑40 (MQ=255) ccAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCg < 1:1093594/71‑1 (MQ=255) | CTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCG > minE/106026‑106163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |