| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,415,838 | G→C | 27.3% | noncoding (41/77 nt) | proM ← | tRNA‑Pro |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,415,838 | 0 | G | C | 27.3% | 82.0 / 27.3 | 44 | noncoding (41/77 nt) | proM | tRNA‑Pro |
| Reads supporting (aligned to +/- strand): ref base G (19/13); new base C (6/6); total (25/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
ACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATACTGCTTTTTGAATTTTTAGTTC > minE/2415770‑2415905 | aCAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTgg > 1:548331/1‑70 (MQ=255) aaaaCCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTccc > 1:3533273/1‑70 (MQ=255) aaaaCCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCa < 1:4770576/71‑1 (MQ=255) gAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTgcg > 1:3122587/1‑71 (MQ=255) gAAGCGGGGTTAAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTgcg > 1:3793516/1‑71 (MQ=255) gCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTgcg > 1:577056/1‑68 (MQ=255) gggTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAAcc < 1:5097850/58‑1 (MQ=255) ttttCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCag > 1:3108423/3‑70 (MQ=12) ttCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCag < 1:2367851/69‑2 (MQ=12) ttCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGc > 1:5014009/1‑71 (MQ=12) ttCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGc > 1:468949/1‑71 (MQ=12) ttCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTgc < 1:4029939/61‑1 (MQ=255) cAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTa > 1:3907592/1‑63 (MQ=255) cAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTg > 1:4132185/1‑71 (MQ=255) aaaaTTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCa < 1:1771869/53‑1 (MQ=255) tttGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTgcgcc < 1:4180925/70‑2 (MQ=16) tttGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTgcgcc < 1:1308051/70‑2 (MQ=16) tttGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTgcg > 1:2772012/2‑69 (MQ=16) tttGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTgcg > 1:1922640/2‑69 (MQ=16) aTTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTa > 1:1358257/1‑59 (MQ=255) aTTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTa > 1:2281846/1‑59 (MQ=255) aTTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTa > 1:4671372/1‑59 (MQ=255) ttGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAAcc < 1:4283347/48‑1 (MQ=255) ttGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAAcc < 1:1723109/48‑1 (MQ=255) ggTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTAc < 1:3823222/57‑1 (MQ=12) ggCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGcc < 1:1147009/71‑1 (MQ=255) gCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGcc > 1:1599202/1‑70 (MQ=255) gatagagGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGaa > 1:1185964/4‑71 (MQ=12) gagagaGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGa > 1:3291001/1‑70 (MQ=255) atagagGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTAc < 1:3747175/47‑1 (MQ=9) gagGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTgcgcc < 1:4244743/57‑2 (MQ=12) cGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATACTGCtttt > 1:2900712/1‑71 (MQ=255) ccGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCt > 1:561022/1‑43 (MQ=255) ccGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTa > 1:2476629/1‑44 (MQ=255) ccGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCg < 1:3706236/51‑1 (MQ=255) ccGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATACTGCTTTTTGAAttt > 1:2233676/1‑71 (MQ=255) cGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATACTGCTTTTTGAAtttt < 1:4269871/71‑1 (MQ=255) cGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATACTGCTTTTTGAAtttt < 1:2270917/71‑1 (MQ=255) cGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATACTGCTTTTTGAAtttt < 1:1273409/71‑1 (MQ=255) gACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGaa > 1:5520604/1‑51 (MQ=255) gACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATACTGCTTTTTGAAtttt > 1:291555/1‑70 (MQ=255) aCTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATAc > 1:3173002/1‑50 (MQ=255) cTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGaaa < 1:4971219/46‑1 (MQ=255) cTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGaaa < 1:2936474/46‑1 (MQ=255) cTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATACTGCTTTTTGAATTTTTAGTTc > 1:1032025/1‑70 (MQ=255) ggTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATa > 1:1049354/1‑46 (MQ=255) ggTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATACTGCTTTTTGAAt > 1:2381708/1‑59 (MQ=255) | ACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGAAATACTGCTTTTTGAATTTTTAGTTC > minE/2415770‑2415905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |