| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,425,818 | C→T | 16.3% | D92N (GAC→AAC) ‡ | rffG ← | dTDP‑glucose 4,6‑dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,425,818 | 0 | C | T | 16.3% | 200.8 / 26.6 | 86 | D92N (GAC→AAC) ‡ | rffG | dTDP‑glucose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (30/42); new base T (4/10); total (34/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01 | |||||||||||
AAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACG > minE/2425749‑2425888 | aaGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTc < 1:1561731/70‑1 (MQ=255)aaGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCCTc > 1:4848317/1‑70 (MQ=255) aGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCaa < 1:5107199/71‑1 (MQ=255) aGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCa < 1:1187261/70‑1 (MQ=255) ccGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAg < 1:3373636/71‑1 (MQ=255) cgcgCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAg < 1:1520510/70‑1 (MQ=255) gcgcCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGaa > 1:2461800/1‑71 (MQ=255) gcgcCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGaa > 1:672439/1‑71 (MQ=255) cTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTc > 1:2357644/1‑70 (MQ=255) cTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTc > 1:4552253/1‑70 (MQ=255) cAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAAc < 1:4946705/70‑1 (MQ=255) cAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAAc < 1:2728608/70‑1 (MQ=255) aTGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTc < 1:4551543/49‑1 (MQ=255) tGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCaa < 1:3042882/50‑1 (MQ=255) tGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCttt > 1:2624697/1‑71 (MQ=255) tataAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCt > 1:977172/1‑67 (MQ=255) tataAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCg > 1:1051877/1‑71 (MQ=255) tataAGTCCCGACAATGTAGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCt > 1:2583339/1‑67 (MQ=255) ataAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCgg > 1:2868128/1‑71 (MQ=255) taAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATg < 1:2085425/62‑1 (MQ=255) aaGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCaa < 1:2888384/57‑1 (MQ=255) aaGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAAc < 1:827688/58‑1 (MQ=255) aGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTg > 1:1189486/1‑71 (MQ=255) aGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGcag > 1:3274020/1‑69 (MQ=255) aGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGca < 1:2768039/70‑2 (MQ=255) aGTACCAACAATATTGGTTTCAATAAATGCCGCACGGCCTGTAATTGAACGGTCAACATGGCTTTCAGca < 1:1468395/70‑2 (MQ=255) tCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGc > 1:2915128/1‑70 (MQ=255) tCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGc > 1:2165144/1‑70 (MQ=255) ccaaCAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAg < 1:4016703/68‑1 (MQ=255) ccGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAg < 1:1554660/71‑1 (MQ=255) gACAATGTTGGTTTCAATGAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGa < 1:2008773/70‑1 (MQ=255) gACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGAt > 1:2648124/1‑71 (MQ=255) aCAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGcc < 1:423368/66‑1 (MQ=255) aCAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGAt > 1:4480877/1‑70 (MQ=255) cAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGc < 1:668410/71‑1 (MQ=255) aaTGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCttt > 1:4805586/1‑56 (MQ=255) aaTGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCa > 1:2081447/1‑71 (MQ=255) aaTGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCa > 1:3004546/1‑71 (MQ=255) aaTGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGGCAACATGGCTTTCGGCTGCCAGATGCa < 1:4994585/71‑1 (MQ=255) tGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCAt > 1:1766674/1‑70 (MQ=255) tGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATg < 1:1381664/71‑1 (MQ=255) tGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATg < 1:1306547/71‑1 (MQ=255) tGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATg < 1:5304892/71‑1 (MQ=255) tGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATg < 1:1075914/71‑1 (MQ=255) ttGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCac > 1:4258597/1‑71 (MQ=255) ttGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGc > 1:199525/1‑56 (MQ=255) ttGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCa < 1:2187802/61‑1 (MQ=255) ggTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCa > 1:5260986/1‑65 (MQ=255) gTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGcag > 1:834593/1‑53 (MQ=255) tttCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCt < 1:2382549/46‑1 (MQ=255) tttCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCt < 1:4754659/46‑1 (MQ=255) tttCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAg < 1:846269/71‑1 (MQ=255) tttCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAg < 1:4314033/71‑1 (MQ=255) tttCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAg < 1:3643897/71‑1 (MQ=255) tCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAgg < 1:4566631/57‑2 (MQ=255) tCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAgg < 1:4306628/57‑2 (MQ=255) tCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAg > 1:5231748/1‑56 (MQ=255) tCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACa > 1:3626959/1‑39 (MQ=255) cAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCt > 1:4457491/1‑71 (MQ=255) aaTAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCt < 1:3736104/70‑1 (MQ=255) aaTAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCt < 1:4878499/70‑1 (MQ=255) aaTAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCt < 1:2843927/70‑1 (MQ=255) aaTAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCt < 1:3665213/70‑1 (MQ=255) aaTAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTg > 1:4198487/1‑71 (MQ=255) aTAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGcag < 1:3045889/49‑3 (MQ=255) aTAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTgg < 1:630480/71‑1 (MQ=255) tAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAggt < 1:2181131/54‑3 (MQ=12) tAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAggt < 1:4471808/54‑3 (MQ=12) aaaCGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCa > 1:1235619/1‑50 (MQ=255) aaaCGCTGCCGGGCCGTCAATAGAACGGTAAACATGGCTTTCGGCTGCCa > 1:5329179/1‑50 (MQ=255) aaCGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCt < 1:1390005/70‑1 (MQ=255) aCGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGa < 1:2618622/71‑1 (MQ=255) cGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGAt > 1:2204662/1‑71 (MQ=255) cGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGAt < 1:1752427/71‑1 (MQ=255) tGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCAt < 1:2917800/51‑1 (MQ=255) tGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCAt < 1:2851498/51‑1 (MQ=255) gggCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGt < 1:4754421/71‑1 (MQ=255) gggCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGGCTGGCTGAt < 1:1568776/64‑1 (MQ=255) ggCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCt < 1:2145930/56‑1 (MQ=255) ggCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTg < 1:12693/71‑1 (MQ=255) ggCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTg < 1:3978996/71‑1 (MQ=255) gCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAg > 1:2883026/1‑68 (MQ=255) gTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTc < 1:2429509/63‑1 (MQ=255) gTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTc < 1:1129379/63‑1 (MQ=255) gTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATa > 1:3336843/1‑71 (MQ=255) tCAATAGTACGGTCAACATGGCTTTCGGCTGCCAGATGCATg < 1:2376346/42‑1 (MQ=255) tCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGt < 1:2928154/65‑1 (MQ=255) tCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTg > 1:2307300/1‑66 (MQ=255) tCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGa > 1:1388735/1‑67 (MQ=255) cAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGATACAGt > 1:4380034/1‑48 (MQ=255) cAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGt > 1:2226470/1‑48 (MQ=255) cAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGt > 1:1421545/1‑48 (MQ=255) cAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATAcg > 1:488319/1‑71 (MQ=255) cAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATAcg > 1:2105939/1‑71 (MQ=255) cAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATAcg > 1:4225083/1‑71 (MQ=255) | AAGCCCGCGCCGCTTCAAGCAATGTATAAGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACG > minE/2425749‑2425888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |