| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,430,365 | T→C | 74.6% | I111M (ATA→ATG) | rfe ← | UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,430,365 | 0 | T | C | 74.6% | 89.7 / 29.5 | 55 | I111M (ATA→ATG) | rfe | UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase |
| Reads supporting (aligned to +/- strand): ref base T (9/5); new base C (24/17); total (33/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
GAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATCCAG > minE/2430299‑2430433 | gAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACcatcat < 1:4688081/69‑1 (MQ=255) tctcCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGcg > 1:1591545/1‑69 (MQ=255) tcCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCaa > 1:3953817/1‑70 (MQ=255) ggAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGACAACAGCgg > 1:1876026/1‑71 (MQ=255) gAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGc < 1:3143576/71‑1 (MQ=255) aGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGcc < 1:4639075/71‑1 (MQ=255) aGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGcc < 1:3735624/71‑1 (MQ=255) aaaGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTa > 1:3041887/1‑71 (MQ=255) aaaGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTa < 1:996059/71‑1 (MQ=255) aaGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTAt < 1:1363460/71‑1 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACa > 1:1682930/1‑59 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACa > 1:3401074/1‑59 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACa > 1:2955528/1‑59 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAg > 1:2353773/1‑60 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAg > 1:2851912/1‑60 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCg < 1:950974/62‑1 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGc > 1:3629612/1‑64 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTAtg < 1:3881189/71‑1 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTAtg > 1:3320812/1‑71 (MQ=255) aGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTAt > 1:3636176/1‑70 (MQ=255) ataACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACa < 1:2839133/46‑1 (MQ=255) cccAGGCTACTGAGATAAAGCTTGCCTAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCAc > 1:4647286/1‑70 (MQ=255) cccAGGCTACTGAGAAAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACg > 1:3595707/1‑71 (MQ=255) ccAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGc > 1:1234656/1‑52 (MQ=255) aGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAt > 1:3704513/1‑71 (MQ=255) aGGCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAt > 1:3746406/1‑71 (MQ=255) gCTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtt < 1:5627160/70‑1 (MQ=255) cTACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtttt < 1:11425/71‑1 (MQ=255) tACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTa > 1:3052358/1‑54 (MQ=255) tACTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtttt > 1:3017120/1‑70 (MQ=255) cTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTAc < 1:2165112/71‑1 (MQ=255) cTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTg < 1:2518382/50‑1 (MQ=255) cTGAGATAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTg < 1:1269056/50‑1 (MQ=255) tGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACg > 1:4908/1‑71 (MQ=255) gagaTAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTAtg > 1:726098/1‑52 (MQ=255) agaTAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGc > 1:152776/1‑44 (MQ=255) gaTAAAGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTg < 1:1980333/46‑1 (MQ=255) aaaGCTTGCCGAACACCATCATAACAATGCCAACAGCgg > 1:190055/1‑39 (MQ=255) aaaGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACg > 1:377346/1‑55 (MQ=255) aaaGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGata > 1:5697217/1‑71 (MQ=255) aaaGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGata > 1:4343376/1‑71 (MQ=255) aaGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGAtttt < 1:44716/60‑1 (MQ=255) aaGCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGata < 1:5457600/70‑1 (MQ=255) gCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTAt < 1:5621114/43‑1 (MQ=255) gCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTAt < 1:1645848/43‑1 (MQ=255) gCTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTAt < 1:267178/43‑1 (MQ=255) cTTGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCaa < 1:129569/71‑1 (MQ=255) tGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTa > 1:1524836/1‑39 (MQ=255) tGCCGAACACCATCATAACAATGCCAACAGCGGCCTGTa > 1:3865971/1‑39 (MQ=255) gCCGAACACCATTATAACACTGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCaaaa < 1:5215364/70‑1 (MQ=255) gCCGAACACCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCaaa < 1:5081049/69‑1 (MQ=255) acacCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCaa > 1:2856093/1‑63 (MQ=255) acacCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCaa > 1:3799802/1‑63 (MQ=255) acacCATCATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCaa > 1:1183979/1‑63 (MQ=255) catcatAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATcc > 1:243200/1‑70 (MQ=255) cATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATc > 1:1072832/1‑69 (MQ=255) aTTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATCCAg > 1:5588071/1‑71 (MQ=255) | GAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAAAGCTTGCCGAACACCATTATAACAATGCCAACAGCGGCCTGTATGGTGGCACGGATTTTTACGCTGATATCAAAACGGTCATCCAG > minE/2430299‑2430433 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |