| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,434,283 | T→C | 5.5% | intergenic (+35/‑101) | rhlB → / → gpp | ATP‑dependent RNA helicase/guanosine pentaphosphatase/exopolyphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,434,283 | 0 | T | C | 5.5% | 207.7 / 2.0 | 73 | intergenic (+35/‑101) | rhlB/gpp | ATP‑dependent RNA helicase/guanosine pentaphosphatase/exopolyphosphatase |
| Reads supporting (aligned to +/- strand): ref base T (38/31); new base C (2/2); total (40/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
CTGGCGCTCCGCGTAATCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGC > minE/2434214‑2434352 | cTGGCGCTCCGCGTAATCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTa > 1:1126626/1‑71 (MQ=255)cTGGCGCTCCGCGTAATCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTa > 1:3085494/1‑71 (MQ=255) gcgcTCCGCGTAATCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATc > 1:4470732/1‑70 (MQ=255) gcgcTCCGCGTAATCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATc < 1:4692085/70‑1 (MQ=255) gcgcTCCGCGTAATCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCa < 1:2443418/71‑1 (MQ=255) cgcTCCGCGTAATCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCaa < 1:3970646/71‑1 (MQ=255) cgcTCCGCGTAATCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCaa < 1:331616/71‑1 (MQ=255) cgcTCCGCGTAATCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCaa < 1:5626912/71‑1 (MQ=255) ccGCGTAATCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATg > 1:1212777/1‑71 (MQ=255) ccGCGTAATCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTCTATCAAGATg > 1:3154847/1‑71 (MQ=255) gTAATCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATc > 1:2390883/1‑61 (MQ=255) aTCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCaa < 1:1692950/60‑1 (MQ=255) aTCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCaa < 1:4820784/60‑1 (MQ=255) aTCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCaa > 1:4905170/1‑68 (MQ=255) aTCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCaa > 1:2752797/1‑68 (MQ=255) tcgtcgtcgTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCaa < 1:883947/59‑1 (MQ=255) tcgtcgtcgTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCaa < 1:349124/59‑1 (MQ=255) gtcgtcgtTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCaa < 1:3866174/57‑1 (MQ=255) gtcgtcgtTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCaa < 1:4599190/57‑1 (MQ=255) tcgtcgtTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAgaatg < 1:2014098/71‑1 (MQ=255) ttCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAgaatga < 1:1491227/66‑1 (MQ=255) ttCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAgaatga < 1:1541547/66‑1 (MQ=255) ttCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATccc > 1:2637809/1‑71 (MQ=255) tCAGGTTAAATAAAAAATGTCATACTCAAAATAATTCAAACTGTATCAAGATGGCAAGAgaatga < 1:4206878/65‑1 (MQ=255) gTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAgaat < 1:1229138/59‑1 (MQ=255) ttAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAgaa > 1:4194020/1‑57 (MQ=255) taaataaaAAATGTCATACTCAAAATCATTCAAACTGTa > 1:1042971/1‑39 (MQ=255) taaataaaAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGc > 1:4197692/1‑71 (MQ=255) taaataaaAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGc > 1:3528107/1‑71 (MQ=255) taaataaaAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCACGATGAGc > 1:5042383/1‑71 (MQ=255) aaataaaAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAgaatgaat > 1:1404664/1‑60 (MQ=255) aaataaaAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCt > 1:4493462/1‑71 (MQ=255) ataaaAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTa < 1:2318112/71‑1 (MQ=255) taaaAAATGTCATACTCAAAATCATTCAAACTGTATCaa < 1:823176/39‑1 (MQ=255) taaaAAATGTCATACTCAAAATCATTCAAACTGTATCaa < 1:4559240/39‑1 (MQ=255) taaaAAATGTCATACTCAAAATCATTCAAACTGTATCaa < 1:3104090/39‑1 (MQ=255) aaaaaTGTCATACTCAAAATCATTCAAACTGTATCa < 1:2357833/36‑1 (MQ=255) aaaaaTGTCATACTCAAAATCATTCAAACTGTATCa < 1:3731587/36‑1 (MQ=255) aaaaaTGTCATACTCAAAATCATTCAAACTGTATCAAGa > 1:175193/1‑39 (MQ=255) aaaaaTGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTg > 1:2179355/1‑71 (MQ=255) aaaaTGTCATACTCAAAATCATTCAAACTGTATCAAg < 1:175639/37‑1 (MQ=255) aTGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTgtag > 1:2023369/1‑70 (MQ=255) tCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTgtagt < 1:1230803/68‑1 (MQ=255) taTACACAAAATCATTCAAGTTGCATCAAGGCGGCAAGTGAGCGAATCCCGATGAGCt < 1:997666/57‑1 (MQ=9) taTACACAAAATCATTCAAGTTGCATCAAGGCGGCAAGTGAGCGAATCCCGATGAGCTTACTCAGGTAAGt > 1:4293028/2‑71 (MQ=9) aTACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAgaa < 1:32071/41‑1 (MQ=255) aTACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGt > 1:5601956/1‑70 (MQ=255) tACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:3953088/1‑71 (MQ=255) tACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:195662/1‑71 (MQ=255) tACACAAAATCATTCAAGTTGCATCAAGGCGGCAAGTGAGCGAATCCCGATGAGCTTACTCAGGTAAGTGa < 1:4024673/71‑1 (MQ=9) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAg > 1:3876870/1‑52 (MQ=255) acAAAATCATTCAAGTTGCATCAAGGCGGCAAGTGAGCGAATCCCGATGAGCTTACTCAGGTAAGTGATTc > 1:2925141/2‑71 (MQ=12) cAAAATCATTCAAACTGTATCAAGATGGCAAGAgaatgaa > 1:5197303/1‑40 (MQ=255) aaaaTCATTCAAACTGTATCAAGATGGCAAGAgaa > 1:3690673/1‑35 (MQ=255) aaaaTCATTCAAACTGTATCAAGATGGCAAGAgaa > 1:5102755/1‑35 (MQ=255) aaaaTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCa < 1:4193246/70‑1 (MQ=255) aaaaTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCa > 1:3893752/1‑70 (MQ=255) aaaaTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAg > 1:2517386/1‑71 (MQ=255) aaaaTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAg > 1:5458564/1‑71 (MQ=255) aaaTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCg < 1:3036918/43‑1 (MQ=255) aaaTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCt < 1:1694394/50‑1 (MQ=255) aTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGAt > 1:4469841/1‑43 (MQ=255) aTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGAtt > 1:4116376/1‑65 (MQ=255) aTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGt > 1:192398/1‑70 (MQ=255) aTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGt > 1:5154368/1‑70 (MQ=255) aTTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTg > 1:2951237/1‑59 (MQ=255) aTTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTg > 1:5533870/1‑59 (MQ=255) aTTCAAACTGTATCAAGATGGCAAGAGAATGAATCACGATGAGCTTACTGTAGTAAGTg > 1:3781978/1‑59 (MQ=255) cAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTgtag > 1:1442162/1‑50 (MQ=255) cAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTgtag > 1:5087141/1‑50 (MQ=255) aaCTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTc < 1:5226499/58‑1 (MQ=255) aaCTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTc < 1:4275211/58‑1 (MQ=255) aaCTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTc < 1:2491927/58‑1 (MQ=255) tATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGAtt < 1:55517/52‑1 (MQ=255) tATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAg < 1:3800996/69‑1 (MQ=255) tATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAg < 1:1632874/69‑1 (MQ=255) tATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGc > 1:1956882/1‑70 (MQ=255) | CTGGCGCTCCGCGTAATCGTCGTCGTTCAGGTTAAATAAAAAATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGC > minE/2434214‑2434352 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |