| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,729 | C→T | 9.9% | V382I (GTC→ATC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,729 | 0 | C | T | 9.9% | 264.6 / 13.1 | 91 | V382I (GTC→ATC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (44/38); new base T (5/4); total (49/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
AACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAG > minE/311659‑311798 | aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc > 1:5483622/1‑71 (MQ=255)aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc > 1:469348/1‑71 (MQ=255)aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc > 1:4240022/1‑71 (MQ=255)aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc > 1:3244469/1‑71 (MQ=255) aGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGc < 1:841289/71‑1 (MQ=255) ggCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:572928/71‑1 (MQ=255) ggCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:4191323/71‑1 (MQ=255) cccGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCaa < 1:1437865/70‑1 (MQ=16) cGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg < 1:1094316/71‑1 (MQ=255) aTGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGt < 1:1344227/71‑1 (MQ=12) tGGCGAGCACCATCCCTAACATTGTTATCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:4981848/65‑1 (MQ=255) ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaaa > 1:5263517/1‑66 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:3276581/71‑1 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:4340837/71‑1 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:1676517/71‑1 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:1865527/71‑1 (MQ=255) aaGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTccct > 1:85032/2‑69 (MQ=12) aGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAg > 1:2721745/1‑71 (MQ=255) aGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTccctaa > 1:1227868/1‑68 (MQ=12) gCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAg > 1:3962273/1‑70 (MQ=255) cACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc > 1:4627673/1‑65 (MQ=255) cACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc > 1:1881803/1‑70 (MQ=255) aCCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTCCCTAACaa < 1:1302844/71‑1 (MQ=12) ccATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCa > 1:2964362/1‑69 (MQ=255) ccATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCa > 1:2161714/1‑69 (MQ=255) ccATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAAt < 1:4201267/71‑1 (MQ=255) ccATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTCCCTAACaac < 1:1962639/71‑2 (MQ=12) cATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc < 1:1733292/67‑1 (MQ=255) cATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTc > 1:128890/1‑61 (MQ=12) cATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTCCCTAACaaca > 1:4166017/1‑69 (MQ=12) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:4952290/54‑1 (MQ=255) tCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTCCCTAACaaca > 1:91188/1‑67 (MQ=12) ccGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc < 1:4266106/63‑1 (MQ=255) gAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg > 1:4877978/1‑71 (MQ=255) aaCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc < 1:2224202/60‑1 (MQ=255) aaCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg < 1:1693640/71‑1 (MQ=255) aCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg > 1:4653480/1‑70 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:1352518/46‑1 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg > 1:1447142/1‑68 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGAGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg > 1:1376820/1‑68 (MQ=255) aTTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTAc < 1:4654934/71‑1 (MQ=255) gTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc > 1:4456264/1‑50 (MQ=255) gTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc > 1:1202656/1‑50 (MQ=255) gTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc > 1:1032302/1‑50 (MQ=255) gTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc > 1:727980/1‑50 (MQ=255) gTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACgg > 1:1724965/1‑70 (MQ=255) tAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCaa > 1:196454/1‑71 (MQ=255) cGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:800495/38‑1 (MQ=255) cGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:4855722/38‑1 (MQ=255) gtgtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg > 1:4259160/1‑39 (MQ=255) gtgtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg > 1:2100296/1‑39 (MQ=255) gtgtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg > 1:5462723/1‑39 (MQ=255) gtgtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg > 1:753075/1‑39 (MQ=255) tgtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc < 1:1681563/47‑1 (MQ=255) gtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgt < 1:4398025/57‑2 (MQ=255) gtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg < 1:3540056/57‑1 (MQ=255) gtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg < 1:2718617/57‑1 (MQ=255) gtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCg > 1:3594483/1‑71 (MQ=255) tttATCGAGAAGCCAAAGGCGGCAAGGACGGCaaa > 1:2446163/1‑35 (MQ=255) tttATCGAGAAGCCAAAGGCGGCAAGGACGGCaaa > 1:2547344/1‑35 (MQ=255) tttATCGAGAAGCCAAAGGCGGCAAGGACGGCaaa > 1:1771258/1‑35 (MQ=255) tttATCGAGAAGCCAAAGGCGGCAAGGACGGCaaa > 1:5407435/1‑35 (MQ=255) tttATCGAGAAGCCAAAGGCGGCAAGGACGGCaaa > 1:5392864/1‑35 (MQ=255) tttATCGAGAAGCCAAAGGCGGCAAGGACGGCaaa > 1:1336855/1‑35 (MQ=255) tttATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg < 1:141612/56‑1 (MQ=255) tttATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCgg < 1:494426/71‑1 (MQ=255) tATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:4050109/39‑1 (MQ=255) aTCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAAt > 1:2996679/1‑71 (MQ=255) tCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATc < 1:2303522/71‑1 (MQ=255) agaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCa < 1:208985/69‑1 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCATCGGTACGGCaa > 1:893220/1‑56 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACg < 1:113161/71‑1 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACg < 1:2329970/71‑1 (MQ=255) aaGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAAc > 1:2228641/1‑69 (MQ=255) aaGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGt > 1:2290489/1‑71 (MQ=255) aaGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAAAACCACCGGTACGGCAATGGTCGGAATCaa > 1:2715058/1‑68 (MQ=255) cAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgcg > 1:2071402/1‑71 (MQ=255) aGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCaa < 1:3393648/48‑1 (MQ=255) aGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCaa < 1:3895571/48‑1 (MQ=255) aGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgcgcg > 1:4846186/1‑70 (MQ=255) aGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCgg < 1:3663923/71‑1 (MQ=255) ggcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGa < 1:2655864/71‑1 (MQ=255) gcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATg > 1:2210611/1‑48 (MQ=255) gcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATg > 1:778627/1‑48 (MQ=255) gcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCACCGTCGCGCgg > 1:3497152/1‑69 (MQ=255) gcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCgg > 1:991608/1‑69 (MQ=255) cAAGGACGGCAAAGGGCCCGAGCAATACCACCGGTACGGCAATGGTCGGa < 1:4347077/50‑1 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt < 1:1388741/71‑1 (MQ=255) aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg < 1:1954891/71‑1 (MQ=255) aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg > 1:2082518/1‑71 (MQ=255) aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg > 1:2930044/1‑71 (MQ=255) aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGGCGGAATCAACGTCGCGCGGAAGTTCTg < 1:1104062/71‑1 (MQ=255) aGGACGGCAAAGGTCCCGAGAAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg > 1:3748501/1‑71 (MQ=255) aCGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAg > 1:612465/1‑71 (MQ=255) aCGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAg > 1:3481233/1‑71 (MQ=255) | AACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAG > minE/311659‑311798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |