| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,434,363 | A→G | 14.5% | intergenic (+115/‑21) | rhlB → / → gpp | ATP‑dependent RNA helicase/guanosine pentaphosphatase/exopolyphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,434,363 | 0 | A | G | 14.5% | 157.5 / 15.6 | 62 | intergenic (+115/‑21) | rhlB/gpp | ATP‑dependent RNA helicase/guanosine pentaphosphatase/exopolyphosphatase |
| Reads supporting (aligned to +/- strand): ref base A (31/22); new base G (4/5); total (35/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
GCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCATTGATCTCGGTTCGAATAG > minE/2434293‑2434433 | gCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga < 1:769099/71‑1 (MQ=255) agagAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAg < 1:4304180/71‑1 (MQ=255) agagAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAg < 1:5298159/71‑1 (MQ=255) agagAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAg < 1:2893665/71‑1 (MQ=255) aatgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGAtt > 1:4042398/1‑70 (MQ=255) aatgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTg < 1:5089868/71‑1 (MQ=255) atgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAg < 1:1837188/66‑1 (MQ=255) atgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTg < 1:1370517/70‑1 (MQ=255) tgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTg < 1:955518/69‑1 (MQ=255) aatCCCGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAg > 1:1530604/1‑70 (MQ=9) aatCCCGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAg > 1:2506493/1‑70 (MQ=9) cccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGa > 1:1279910/1‑69 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg < 1:3958831/70‑1 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg < 1:121317/70‑1 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg > 1:594413/1‑70 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg > 1:3322455/1‑70 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGGGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg < 1:2059265/70‑1 (MQ=255) ccGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg > 1:3565310/1‑70 (MQ=9) ccGATGAGCTCACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg < 1:4671174/70‑1 (MQ=255) cGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa < 1:1773347/71‑1 (MQ=255) aTGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa > 1:5325034/1‑69 (MQ=255) aTGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg < 1:1081083/67‑1 (MQ=9) aTGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg < 1:128231/67‑1 (MQ=9) aTGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg < 1:1367101/67‑1 (MQ=9) tGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag < 1:564370/71‑1 (MQ=255) tGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag < 1:5655139/71‑1 (MQ=255) tGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag < 1:3058619/71‑1 (MQ=255) gAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGCGACAGATTGAAGGATGAagag > 1:2168033/1‑70 (MQ=255) gAGCTTACTCAGGTAAGTGATTCGTGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAagtg > 1:3677193/1‑68 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTa > 1:3899367/1‑71 (MQ=255) aGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAgtgt < 1:2636176/70‑1 (MQ=9) aCTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga > 1:4518654/1‑45 (MQ=255) aCTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga > 1:3698710/1‑45 (MQ=255) aCTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTa > 1:5067942/1‑66 (MQ=255) tagtaAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCa > 1:2404347/1‑71 (MQ=255) aGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGa > 1:1790166/1‑48 (MQ=255) tGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCtcgtc > 1:1550582/1‑71 (MQ=255) tGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCtcgtc > 1:2891341/1‑71 (MQ=255) tGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCtcgtc > 1:2311966/1‑71 (MQ=255) tGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATCAAGAGTATGGGTTCCACAtcgtc > 1:5458551/1‑71 (MQ=255) gATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCtcgtcg > 1:4667734/1‑71 (MQ=255) ttCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa < 1:3243562/47‑1 (MQ=255) ttCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa < 1:1675654/47‑1 (MQ=255) ttCAGGGGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa < 1:3009514/47‑1 (MQ=255) cAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTg < 1:5350950/70‑1 (MQ=255) aGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGt < 1:2491598/70‑1 (MQ=255) tGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa > 1:3101460/1‑41 (MQ=255) tGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGc < 1:3294857/71‑1 (MQ=255) gAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg > 1:3942289/1‑49 (MQ=255) gAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg > 1:1985509/1‑49 (MQ=255) aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg > 1:4383222/1‑48 (MQ=255) aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg > 1:962305/1‑48 (MQ=255) aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg > 1:1439269/1‑48 (MQ=255) aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg > 1:922984/1‑48 (MQ=255) aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg > 1:5610772/1‑48 (MQ=255) aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATgg > 1:4693506/1‑47 (MQ=255) aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATgg > 1:3802152/1‑47 (MQ=255) gAATGCAGCCAACACAGAGAGAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCa > 1:4839376/1‑71 (MQ=255) gAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCa > 1:1017520/1‑71 (MQ=255) gAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCa > 1:1944370/1‑71 (MQ=255) aaTGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCt > 1:2739377/1‑52 (MQ=255) aaTGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCt > 1:2340350/1‑52 (MQ=255) aaTGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCt > 1:2115110/1‑52 (MQ=255) tGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCATTg < 1:2741606/71‑1 (MQ=255) gCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCATTg < 1:4640623/70‑1 (MQ=255) gagaCAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCt > 1:4109164/1‑44 (MQ=255) gagaCAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCATTGATCTCGGTTCGaa < 1:2745846/71‑1 (MQ=255) gagaCAGATTGAAGGATGAAGAGTATGGGTTCCAACTCGTCGc > 1:2279848/1‑43 (MQ=255) aCAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCATTGATCTCGGTTCGAATAg > 1:1756418/1‑71 (MQ=255) | GCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCATTGATCTCGGTTCGAATAG > minE/2434293‑2434433 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |