Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,451,489 A→C 14.6% intergenic (+2/+94) hdfR → / ← trpT DNA‑binding transcriptional regulator/tRNA‑Trp

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,451,4890AC14.6% 81.5 / 11.6 41intergenic (+2/+94)hdfR/trpTDNA‑binding transcriptional regulator/tRNA‑Trp
Reads supporting (aligned to +/- strand):  ref base A (15/20);  new base C (3/3);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

AAAATAGCGATAAAAATGCGTTGATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGTTATTTCAGATAAAAAAAATCC  >  minE/2451420‑2451558
                                                                     |                                                                     
aaaaTAGCGATAAAAATGCGTTGATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAatat                                                                      >  1:5060283/1‑71 (MQ=255)
            aaaaTGCGTTGATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAAtggctggc                                                           >  1:1484428/1‑70 (MQ=255)
            aaaaTGCGTTGATTCGCGATCTATTGAAAATTAACGTGCTGGATGAAGTGTATTAATCTGAAtggctggc                                                           <  1:4973114/70‑1 (MQ=255)
            aaaaTGCGTTGATTCGCGATCTATTGAAAATTAACGTGCTGGATGAAGTGTATTAATCTGAAtggctggc                                                           <  1:5594853/70‑1 (MQ=255)
            aaaaTGCGTTGATTCGCGATCTATTGAAAATTAACGTGCTGGATGAAGTGTATTAATCTGAAtggctggc                                                           <  1:1891037/70‑1 (MQ=255)
              aaTGCGTTGATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAg                                                        <  1:1164958/71‑1 (MQ=255)
              aaTGCGTTGATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAg                                                        <  1:1732527/71‑1 (MQ=255)
              aaTGCGTTGATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATAAATATGAATGGCTGGCAAg                                                        <  1:1728646/71‑1 (MQ=255)
               aTGCGTTGATTCGCGATCTATTGAAAATTAACGTGCTGGATGAAGTGTATTAATCTGAAtggctg                                                             >  1:961039/1‑65 (MQ=255)
               aTGCGTTGATTCGCGATCTATTGAAAATTAACGTGCTGGATGAAGTGTATTAATCTGAAtggctg                                                             >  1:3787375/1‑65 (MQ=255)
               aTGCGTTGATTCGCGATCTATTGAAAATTAACGTGCTGGATGAAGTGTATTAATCTGAAtggctg                                                             >  1:3977220/1‑65 (MQ=255)
                 gCGTTGATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAAtgg                                                                <  1:1304831/60‑1 (MQ=255)
                    ttGATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCaa                                                         <  1:1879326/64‑1 (MQ=255)
                    ttGATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCaa                                                         <  1:5255762/64‑1 (MQ=255)
                     tGATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCg                                                 >  1:3999197/1‑71 (MQ=255)
                      gATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAata                                                                       >  1:5624068/1‑48 (MQ=255)
                      gATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGaa                                                                   >  1:5134135/1‑52 (MQ=255)
                      gATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGaa                                                                   >  1:3279930/1‑52 (MQ=255)
                       aTTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGa                                                      >  1:3212467/1‑64 (MQ=255)
                       aTTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGa                                                      >  1:938753/1‑64 (MQ=255)
                        ttCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAata                                                                       <  1:454889/46‑1 (MQ=255)
                          cgcgATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAg                                             >  1:2802642/1‑70 (MQ=255)
                           gcgATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAGaa                                           <  1:3850691/71‑1 (MQ=255)
                                   ttGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGa                                        <  1:2004013/66‑1 (MQ=255)
                                   ttGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGAt                                       <  1:107086/67‑1 (MQ=255)
                                     gAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACtt                                 >  1:3450058/1‑71 (MQ=255)
                                      aaaaTTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCTGTAGAAGGATTTACTTc                                <  1:4342026/71‑1 (MQ=255)
                                      aaaaTTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACtt                                 <  1:1111467/70‑1 (MQ=255)
                                      aaaaTTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTc                                <  1:2947568/71‑1 (MQ=255)
                                         aTTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAgg                                                       <  1:3989471/45‑1 (MQ=255)
                                         aTTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAgg                                                       <  1:5438908/45‑1 (MQ=255)
                                         aTTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAg                                             <  1:1045218/55‑1 (MQ=255)
                                          ttAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCaa                                                         >  1:567658/1‑42 (MQ=255)
                                             aCGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGagag                          <  1:4071144/70‑1 (MQ=255)
                                                  cTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGTTAt                    <  1:1181574/71‑1 (MQ=255)
                                                        gAAGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGt                       <  1:3686127/62‑1 (MQ=255)
                                                         aaGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGTTATTTCAGa              >  1:4615895/1‑70 (MQ=255)
                                                            tctATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGTTATTTCAGATaa           >  1:594107/3‑70 (MQ=255)
                                                              tATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCgg                              >  1:1172623/1‑49 (MQ=255)
                                                              tATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGTTATTTCAg               >  1:5062031/1‑64 (MQ=255)
                                                              tATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGTTATTTCAGATaaaaa        <  1:684635/71‑1 (MQ=255)
                                                              tATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGTTATTTCAGATaaaaa        <  1:691412/71‑1 (MQ=255)
                                                              tATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGTTATTTCAGATaaaa         >  1:4415928/1‑70 (MQ=255)
                                                                ttAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGTTATTTCAGATaaaaaa       >  1:1566868/1‑70 (MQ=255)
                                                                   atatGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGTTATTTCAGATAAAAAAAATc   >  1:4968400/1‑71 (MQ=255)
                                                                   atatGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGTTATTTCAGATAAAAAAAATc   >  1:3254616/1‑71 (MQ=255)
                                                                     atGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGTTATTTCAGATAAAAAAAATcc  >  1:203125/1‑70 (MQ=255)
                                                                     |                                                                     
AAAATAGCGATAAAAATGCGTTGATTCGCGATCTTTTGAAAATTAACGTGCTGGATGAAGTGTATTAATATGAATGGCTGGCAAGGATGCCGGTAGAAGGATTTACTTCGGAGAGGGTTATTTCAGATAAAAAAAATCC  >  minE/2451420‑2451558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: