| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,493,675 | C→T | 18.6% | intergenic (+57/‑318) | gyrB → / → ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,493,675 | 0 | C | T | 18.6% | 204.8 / 31.6 | 86 | intergenic (+57/‑318) | gyrB/ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Reads supporting (aligned to +/- strand): ref base C (28/42); new base T (9/7); total (37/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
ATATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGA > minE/2493605‑2493741 | atatCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATc > 1:3449625/1‑71 (MQ=255) tCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCtt > 1:1967521/1‑70 (MQ=255) tCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCtt < 1:1940002/70‑1 (MQ=255) tCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTg < 1:4865322/71‑1 (MQ=255) cGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCtt > 1:1070304/1‑69 (MQ=255) gcgcTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGaa < 1:880253/70‑1 (MQ=255) gcgcTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGaa < 1:4091137/70‑1 (MQ=255) gcgcTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAAt < 1:227000/71‑1 (MQ=255) cgcTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAAtt < 1:4010695/71‑1 (MQ=255) gcTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAAtt < 1:4099130/70‑1 (MQ=255) aCCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGt < 1:4542828/58‑1 (MQ=255) aTGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAt < 1:2327127/71‑1 (MQ=255) tGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTc < 1:633648/65‑1 (MQ=255) tGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTc < 1:3570730/65‑1 (MQ=255) gAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg < 1:4002687/71‑1 (MQ=255) gAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg < 1:4009331/71‑1 (MQ=255) gCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTa > 1:2053843/1‑50 (MQ=255) gCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTa > 1:2254195/1‑50 (MQ=255) tGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTc < 1:3892662/57‑1 (MQ=255) tGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTc < 1:5127138/57‑1 (MQ=255) ccTGATGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATg > 1:2928634/1‑43 (MQ=14) ccTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg < 1:1247188/64‑1 (MQ=255) cTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGaa < 1:2609100/49‑1 (MQ=255) cTGATGCGCTACGCTTATCAGGACTACGAAAATCTTGCAATGTATTGaa < 1:2547853/49‑1 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATg < 1:5612276/41‑1 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGaa < 1:4711895/48‑1 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGAAAATCTTGc < 1:5310180/36‑1 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGAAAATCTTGc < 1:5587305/36‑1 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGAAAATCTTGc < 1:1947356/36‑1 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGAAAATCTTGc < 1:3508759/36‑1 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGAAAATCTTGc < 1:3413032/36‑1 (MQ=255) aTGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGAt < 1:254560/70‑1 (MQ=14) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGc > 1:880369/1‑35 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTg > 1:4193514/1‑44 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTg > 1:1971126/1‑44 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTg > 1:3670612/1‑44 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGAt < 1:4306832/70‑1 (MQ=255) tGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGAtt > 1:4838595/1‑56 (MQ=14) tGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGAtt > 1:1173598/1‑56 (MQ=14) tGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAtttt > 1:3610521/1‑58 (MQ=255) tGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAtttt > 1:3730010/1‑58 (MQ=255) gcgcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATaa < 1:3365579/70‑1 (MQ=255) cgcTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTa > 1:2611704/1‑38 (MQ=17) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCg < 1:2182818/71‑1 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCg > 1:3665076/1‑71 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCg < 1:2305677/71‑1 (MQ=255) tACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGaa < 1:4754536/40‑1 (MQ=255) tACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGt > 1:3218808/1‑71 (MQ=255) ccGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGc > 1:2676404/2‑58 (MQ=14) cGCTTATCAGGCCTACGAAAATTCTGCAATTTATTGAATTTGCACGATTTTGTa < 1:983921/54‑1 (MQ=11) cGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCa > 1:3049601/1‑44 (MQ=11) cGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTa < 1:1981738/54‑1 (MQ=14) gCTTATCAGGCCTACGAAAATCTTGCAATGTATTGaa < 1:1402563/37‑1 (MQ=255) gCTTATCAGGCCTACGAAAATCTTGCAATGTATTGaa < 1:698211/37‑1 (MQ=255) gCTTATCAGGCCTACGAAAATCTTGCAATGTATTGaa < 1:5158473/37‑1 (MQ=255) gCTTATCAGGCCTACGAAAATCTTGCAATGTATTGaa < 1:4811310/37‑1 (MQ=255) ttATCAGGCCTACGAAAATCTTGCAATGTATTGAAtt > 1:3932463/1‑37 (MQ=255) aTCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGTTAAcg < 1:3397947/69‑1 (MQ=14) cAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTg > 1:5193231/1‑45 (MQ=14) cAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTg > 1:3333938/1‑45 (MQ=14) aGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGTTAACGCCGCa < 1:4731642/71‑1 (MQ=14) aGGCCTACGAAAATCTTGCAATGTATTGAATTTTTATGATTTTGTAGGc > 1:4527492/1‑49 (MQ=255) cTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGtt < 1:3876547/58‑1 (MQ=11) cTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGTTAACGCCGCATcc < 1:1039345/70‑1 (MQ=11) cTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCCg > 1:2040621/1‑71 (MQ=11) cTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTa > 1:3301491/1‑42 (MQ=255) cTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATcc < 1:5466208/70‑1 (MQ=255) tACGAAAATCTTGCAATGTATTGAATTTTCATGAttt < 1:4736070/37‑1 (MQ=255) tACGAAAATCTTGCAATGTATTGAATTTTCATGAttt < 1:679215/37‑1 (MQ=255) tACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAcgccgc < 1:4336483/65‑1 (MQ=255) tACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAc < 1:3905775/60‑1 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGAtttt > 1:1183731/1‑36 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGAtttt > 1:87395/1‑36 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGAtttt > 1:2569587/1‑36 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAcgc > 1:4880723/1‑60 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAcgc > 1:4238418/1‑60 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:2333479/64‑1 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:3010509/1‑70 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:3104643/1‑70 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:2170843/1‑70 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:4910070/1‑70 (MQ=255) aaaaTCTTGCAATGTATTGAATTTTCATGATTTTGTa > 1:2625088/1‑37 (MQ=255) aaaaTCTTGCAATGTATTGAATTTTCATGATTTTGTa > 1:1563655/1‑37 (MQ=255) aaaaTCTTGCAATGTATTGAATTTTCATGATTTTGTa > 1:2374485/1‑37 (MQ=255) aaaTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaaga < 1:1621509/71‑1 (MQ=255) aTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCg < 1:3741076/48‑1 (MQ=255) aTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAcgcc > 1:4676899/1‑56 (MQ=255) cTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGtt > 1:2264722/1‑48 (MQ=255) | ATATCGATATTTAATGGCGCTAACCATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGA > minE/2493605‑2493741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |