| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,493,697 | T→C | 19.4% | intergenic (+79/‑296) | gyrB → / → ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,493,697 | 0 | T | C | 19.4% | 152.9 / 26.4 | 67 | intergenic (+79/‑296) | gyrB/ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Reads supporting (aligned to +/- strand): ref base T (32/22); new base C (6/7); total (38/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01 | |||||||||||
ATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGAAAAGGGCGGAGATCA > minE/2493630‑2493766 | aTGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAt < 1:2327127/71‑1 (MQ=255) gAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg < 1:4009331/71‑1 (MQ=255) gAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg < 1:4002687/71‑1 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTg < 1:1247188/64‑1 (MQ=255) aTGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGAt < 1:254560/70‑1 (MQ=14) aTGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGAt < 1:4306832/70‑1 (MQ=255) tGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGAtt > 1:1173598/1‑56 (MQ=14) tGCGCTCCGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGAtt > 1:4838595/1‑56 (MQ=14) tGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAtttt > 1:3730010/1‑58 (MQ=255) tGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGAtttt > 1:3610521/1‑58 (MQ=255) gcgcTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATaa < 1:3365579/70‑1 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCg > 1:3665076/1‑71 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCg < 1:2182818/71‑1 (MQ=255) cTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCg < 1:2305677/71‑1 (MQ=255) tACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGt > 1:3218808/1‑71 (MQ=255) ccGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGc > 1:2676404/2‑58 (MQ=14) cGCTTATCAGGCCTACGAAAATTCTGCAATTTATTGAATTTGCACGATTTTGTa < 1:983921/54‑1 (MQ=11) cGCTTATCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTa < 1:1981738/54‑1 (MQ=14) aTCAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGTTAAcg < 1:3397947/69‑1 (MQ=14) cAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTg > 1:3333938/1‑45 (MQ=14) cAGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTg > 1:5193231/1‑45 (MQ=14) aGGCCTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGTTAACGCCGCa < 1:4731642/71‑1 (MQ=14) aGGCCTACGAAAATCTTGCAATGTATTGAATTTTTATGATTTTGTAGGc > 1:4527492/1‑49 (MQ=255) cTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGtt < 1:3876547/58‑1 (MQ=11) cTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGTTAACGCCGCATcc < 1:1039345/70‑1 (MQ=11) cTACGAAAATTCTGCAATGTATTGAATTTGCACGATTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCCg > 1:2040621/1‑71 (MQ=11) cTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTa > 1:3301491/1‑42 (MQ=255) cTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATcc < 1:5466208/70‑1 (MQ=255) tACGAAAATCTTGCAATGTATTGAATTTTCATGAttt < 1:4736070/37‑1 (MQ=255) tACGAAAATCTTGCAATGTATTGAATTTTCATGAttt < 1:679215/37‑1 (MQ=255) tACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAcgccgc < 1:4336483/65‑1 (MQ=255) tACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAc < 1:3905775/60‑1 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGAtttt > 1:1183731/1‑36 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGAtttt > 1:87395/1‑36 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGAtttt > 1:2569587/1‑36 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAcgc > 1:4238418/1‑60 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAcgc > 1:4880723/1‑60 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:2333479/64‑1 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:4910070/1‑70 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:2170843/1‑70 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:3010509/1‑70 (MQ=255) cGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc > 1:3104643/1‑70 (MQ=255) aaaaTCTTGCAATGTATTGAATTTTCATGATTTTGTa > 1:2625088/1‑37 (MQ=255) aaaaTCTTGCAATGTATTGAATTTTCATGATTTTGTa > 1:2374485/1‑37 (MQ=255) aaaaTCTTGCAATGTATTGAATTTTCATGATTTTGTa > 1:1563655/1‑37 (MQ=255) aaaTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaaga < 1:1621509/71‑1 (MQ=255) aTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCg < 1:3741076/48‑1 (MQ=255) aTCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAcgcc > 1:4676899/1‑56 (MQ=255) cTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGtt > 1:2264722/1‑48 (MQ=255) ttGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaagaag > 1:2946024/1‑68 (MQ=255) ttGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaagaag > 1:4330642/1‑68 (MQ=255) ttGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaagaa > 1:462593/1‑67 (MQ=255) cAATGTATTGAATTTGCATGATTTTGTAGGCCGGATAAGGCGTTCAc < 1:3526024/47‑1 (MQ=255) tGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGaa < 1:3060236/71‑1 (MQ=255) tATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGaa > 1:3563597/1‑69 (MQ=255) ttGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAc < 1:3786309/40‑1 (MQ=40) ttGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGaaa < 1:244967/68‑1 (MQ=255) ttGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGAAAAgg > 1:2694043/1‑71 (MQ=255) gAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGaaa > 1:426694/1‑66 (MQ=255) gAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGAAAAgg > 1:3792377/1‑69 (MQ=255) gAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGAAAAgg > 1:3051503/1‑69 (MQ=255) aaTTTTCATGATTTTGTAGGCCGGATAAGGCGTTCAc < 1:2969355/37‑1 (MQ=40) ttCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGaa > 1:331819/1‑60 (MQ=255) ttCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGAAAAGGGCg > 1:4111671/1‑67 (MQ=255) ttCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGAAAAGGGCg > 1:513090/1‑67 (MQ=255) cATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGAAAAGGGCGGAGat < 1:3827537/70‑1 (MQ=255) aTGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGAAAAGGGCGGAGatca > 1:2543330/1‑71 (MQ=255) tGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGaaa > 1:1319425/1‑57 (MQ=255) | ATGCGAGCGTGCCTGATGCGCTACGCTTATCAGGCCTACGAAAATCTTGCAATGTATTGAATTTTCATGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAGCTATAAGAAAAGGGCGGAGATCA > minE/2493630‑2493766 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |