| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,493,839 | A→C | 66.7% | intergenic (+221/‑154) | gyrB → / → ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,493,839 | 0 | A | C | 66.7% | 80.8 / 40.5 | 57 | intergenic (+221/‑154) | gyrB/ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Reads supporting (aligned to +/- strand): ref base A (7/12); new base C (24/14); total (31/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.07e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01 | |||||||||||
TTTTATTTCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGATGAG > minE/2493777‑2493908 | ttttATTTCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGaaaaaaa < 1:5524626/69‑1 (MQ=255) tttCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAg < 1:304583/67‑1 (MQ=255) cAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGAt > 1:4766206/1‑70 (MQ=255) gCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGaaaaaaa < 1:92813/52‑1 (MQ=255) gCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGaaaaaaa < 1:3406183/52‑1 (MQ=255) gCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGaaaaaaa < 1:4902129/52‑1 (MQ=255) cAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCtt > 1:3364734/1‑70 (MQ=255) cAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCtt > 1:746169/1‑70 (MQ=255) aaTTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGaaaaaaa < 1:5593102/50‑1 (MQ=255) aaTTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGaaaaaaa < 1:5646675/50‑1 (MQ=255) ttATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGaaaaaaaaa > 1:3743267/1‑50 (MQ=255) ttATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGaaaaaaaaa > 1:4246022/1‑50 (MQ=255) ttATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGaaaaaaa < 1:4793362/48‑1 (MQ=255) ttATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGaaa < 1:5258143/71‑1 (MQ=255) atcatcCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAg > 1:2047740/1‑71 (MQ=255) atcatcCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGATGATAAGGCTTGAAAAg > 1:403524/1‑71 (MQ=255) gCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTc < 1:3809274/71‑1 (MQ=255) aTCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGAt < 1:4985623/46‑1 (MQ=255) tactaTGCAATAGACCATAAACTGAAAAAAAAAGTCCGCt < 1:3912234/40‑1 (MQ=255) tctaTGCAATAGACCATAAACTGCAAAAAAAAGTCCGCTGAt < 1:755670/41‑1 (MQ=39) tctaTGCAATAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTg < 1:4627031/49‑1 (MQ=255) ctaTGCAATAGACCATAAACTGAAAAAAAAAGTcc > 1:2019454/1‑35 (MQ=255) ctaTCCAATAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCAtt < 1:2535585/60‑1 (MQ=255) taTGCAATAGACCATAAACTGCAAAAAAAAGTCCGCt < 1:2071256/37‑1 (MQ=255) taTGCAATAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGaaaa > 1:940286/1‑52 (MQ=255) taTGCAATAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGaaaa > 1:4678241/1‑52 (MQ=255) taTGCAATAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGaaaa > 1:1630552/1‑52 (MQ=255) taTGCAATAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAtt > 1:1796049/1‑71 (MQ=255) tGCAATAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGc > 1:4875959/1‑43 (MQ=255) tGCAATAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGaaa > 1:2337316/1‑49 (MQ=255) tGCAATAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCAttt > 1:2201665/1‑58 (MQ=255) tGCAATAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCAttt > 1:2624954/1‑58 (MQ=255) tGCAATAGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAtttt > 1:2403760/1‑71 (MQ=255) tGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAtttt < 1:1637716/71‑1 (MQ=255) tGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAtttt < 1:2475266/71‑1 (MQ=255) gCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAtttt < 1:123907/70‑1 (MQ=255) tAGACCATAAACTGCAAAAAAAAGTGCGCTGATAAGGCTTGAAAAGTTCATTTcc > 1:4811540/1‑55 (MQ=255) aGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGaaaa > 1:655484/1‑44 (MQ=255) aGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAg > 1:3376410/1‑45 (MQ=255) aGACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGt > 1:899895/1‑46 (MQ=255) gACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACAt > 1:2698320/1‑69 (MQ=255) gACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCg > 1:4049814/1‑71 (MQ=255) gACCATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCg < 1:351272/71‑1 (MQ=255) gACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTc < 1:3126821/47‑1 (MQ=255) aCCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTc < 1:2879620/46‑1 (MQ=255) ccATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTcc < 1:4765338/51‑1 (MQ=255) cATAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAg > 1:2865444/1‑71 (MQ=255) aTAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCAt < 1:5141393/45‑1 (MQ=255) aTAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTc < 1:2597/48‑1 (MQ=255) tAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTc < 1:5494310/47‑1 (MQ=255) tAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGa > 1:215284/1‑51 (MQ=255) tAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGa > 1:544089/1‑51 (MQ=255) tAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGcc > 1:4767103/1‑71 (MQ=255) tAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGcc > 1:2445544/1‑71 (MQ=255) tAAACTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGcc < 1:610029/71‑1 (MQ=255) tAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGcc < 1:5076355/71‑1 (MQ=255) aaaCTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGt > 1:5436634/1‑66 (MQ=255) aaaCTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGt > 1:4879446/1‑66 (MQ=255) aaaCTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGt > 1:1320467/1‑66 (MQ=255) aaaCTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGcc > 1:3485064/1‑70 (MQ=255) aaCTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGa > 1:2564465/1‑71 (MQ=255) aCTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGAt < 1:3815779/71‑1 (MQ=255) cTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTc < 1:1344305/38‑1 (MQ=255) cTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTc < 1:86518/38‑1 (MQ=255) cTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGATg < 1:5609190/71‑1 (MQ=255) cTGCAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGATg < 1:2717303/71‑1 (MQ=255) aaaaaaaaaGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGATGAg < 1:5433787/70‑1 (MQ=255) aaaaaaaaaGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGATGAg > 1:3093515/1‑70 (MQ=255) | TTTTATTTCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTGAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGATGAG > minE/2493777‑2493908 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |