BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,562,526	C→T	8.8%	E517E (GAG→GAA)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,562,526	0	C	T	8.8%	355.2 / 16.6	125	E517E (GAG→GAA)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base C (56/58); new base T (4/7); total (60/65)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 128 total aligned reads displayed.

GCGTGGTGATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGT  >  minE/2562456‑2562594

gCGTGGTGATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGAc                                                                      <  1:2810540/71‑1 (MQ=255)

  gtggtgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGActc                                                                    <  1:34773/71‑1 (MQ=255)

  gtggtgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGAatc                                                                    >  1:2799826/1‑71 (MQ=255)

    ggtgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCtt                                                                  >  1:4011431/1‑71 (MQ=255)

     gtgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCttt                                                                 >  1:1093471/1‑71 (MQ=255)

     gtgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCttt                                                                 >  1:2627907/1‑71 (MQ=255)

      tgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTg                                                                <  1:4280584/71‑1 (MQ=255)

       gATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGc                                                               >  1:3097850/1‑71 (MQ=255)

        aTCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGcc                                                              >  1:5326431/1‑71 (MQ=255)

        aTCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGcc                                                              >  1:1408164/1‑71 (MQ=255)

         tCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGccc                                                             <  1:2682002/71‑1 (MQ=255)

           aCGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccac                                                           <  1:5276933/71‑1 (MQ=255)

            acgccACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccac                                                           <  1:4110805/67‑1 (MQ=255)

             gCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccac                                                           >  1:2225579/1‑69 (MQ=255)

                 aCTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGcc                                                     <  1:250831/71‑1 (MQ=255)

                 aCTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGcc                                                     >  1:721085/1‑71 (MQ=255)

                  cTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAg                                                        <  1:3891471/67‑1 (MQ=255)

                  cTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGc                                                      <  1:3262212/69‑1 (MQ=255)

                   tataCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTa                                                   >  1:2252248/1‑71 (MQ=255)

                    ataCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccacca                                                         >  1:2364439/1‑64 (MQ=255)

                     taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAcc                                                 >  1:286219/1‑71 (MQ=255)

                     taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAcc                                                 >  1:1960819/1‑71 (MQ=255)

                     taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAc                                                  >  1:3215675/1‑70 (MQ=255)

                       cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                <  1:4714091/70‑1 (MQ=255)

                       cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                <  1:624641/70‑1 (MQ=255)

                         ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                             <  1:2667496/71‑1 (MQ=255)

                         ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                             <  1:4217252/71‑1 (MQ=255)

                           tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                           <  1:756139/71‑1 (MQ=255)

                           tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                           <  1:398186/71‑1 (MQ=255)

                           tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                           >  1:4525704/1‑71 (MQ=255)

                            gAAGATCAGGCTGTGTTTGACATTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                           >  1:4322132/1‑70 (MQ=255)

                               gATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                        <  1:1825952/70‑1 (MQ=255)

                                aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                             <  1:1260010/64‑1 (MQ=255)

                                aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                      <  1:1243591/71‑1 (MQ=255)

                                 tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccacca                                                         >  1:5355154/1‑51 (MQ=255)

                                 tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccacca                                                         >  1:5674764/1‑51 (MQ=255)

                                 tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                      >  1:4296573/1‑70 (MQ=255)

                                  cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGc                                                               >  1:2708075/1‑44 (MQ=255)

                                  cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGc                                                               >  1:1588066/1‑44 (MQ=255)

                                  cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                    >  1:772871/1‑71 (MQ=255)

                                  cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                    >  1:3764316/1‑71 (MQ=255)

                                  cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                    >  1:4599883/1‑71 (MQ=255)

                                  cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                    >  1:5518617/1‑71 (MQ=255)

                                     gCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatag                                 <  1:4509397/71‑1 (MQ=255)

                                      cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                <  1:3656059/71‑1 (MQ=255)

                                         tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgat                             <  1:1353602/71‑1 (MQ=255)

                                         tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                              <  1:5121931/70‑1 (MQ=255)

                                         tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                   <  1:3451277/65‑1 (MQ=255)

                                          gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                              >  1:1374445/1‑69 (MQ=255)

                                          gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                              >  1:169429/1‑69 (MQ=255)

                                          gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                     <  1:1202237/62‑1 (MQ=255)

                                          gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                     <  1:2046781/62‑1 (MQ=255)

                                          gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                     <  1:1320160/62‑1 (MQ=255)

                                           tttGACAGTAAAGCTGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                            <  1:2911125/70‑1 (MQ=255)

                                           tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                              >  1:3311967/1‑68 (MQ=255)

                                           tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGcgatagcgatag                           <  1:4068233/71‑1 (MQ=255)

                                             tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                           >  1:606529/1‑53 (MQ=255)

                                             tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                           >  1:1462011/1‑53 (MQ=255)

                                             tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                         >  1:1335316/1‑71 (MQ=255)

                                              caCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                         >  1:4225911/2‑70 (MQ=255)

                                               aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                        <  1:581073/70‑1 (MQ=255)

                                               aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                        <  1:2627415/70‑1 (MQ=255)

                                                 aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                      >  1:5369751/1‑54 (MQ=255)

                                                 aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                     <  1:4966100/71‑1 (MQ=255)

                                                 aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                     <  1:1487587/71‑1 (MQ=255)

                                                  gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACcgccgc                                            <  1:678832/47‑1 (MQ=255)

                                                  gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                    <  1:93396/71‑1 (MQ=255)

                                                   tAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                            <  1:4692752/62‑1 (MQ=255)

                                                     aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgggg                  <  1:1311113/70‑1 (MQ=255)

                                                      aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgata                                  <  1:980012/53‑1 (MQ=255)

                                                      aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                   >  1:5267372/1‑68 (MQ=255)

                                                       gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCggg                   >  1:296299/1‑67 (MQ=255)

                                                       gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGgag                <  1:3349636/70‑1 (MQ=255)

                                                       gCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                   >  1:156403/1‑67 (MQ=255)

                                                        cGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat              <  1:3629734/71‑1 (MQ=255)

                                                         ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat              >  1:5483102/1‑70 (MQ=255)

                                                         ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat              >  1:702624/1‑70 (MQ=255)

                                                         ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat              >  1:2565244/1‑70 (MQ=255)

                                                          gAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca            >  1:856635/1‑71 (MQ=255)

                                                           aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcac                                          <  1:4887408/40‑1 (MQ=255)

                                                           aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                    <  1:1567484/46‑1 (MQ=255)

                                                           aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca            <  1:518136/70‑1 (MQ=255)

                                                            aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                    >  1:4716623/1‑45 (MQ=255)

                                                             cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                        >  1:2525723/1‑40 (MQ=255)

                                                             cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                   <  1:3740631/61‑1 (MQ=255)

                                                              aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                            <  1:1256531/35‑1 (MQ=255)

                                                              aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                            <  1:2488766/35‑1 (MQ=255)

                                                              aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                            <  1:5156539/35‑1 (MQ=255)

                                                               aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa       >  1:3274186/1‑71 (MQ=255)

                                                               aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa       >  1:753411/1‑71 (MQ=255)

                                                                ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                     <  1:1172219/55‑1 (MQ=255)

                                                                ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc      <  1:3223874/70‑3 (MQ=255)

                                                                ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg       <  1:3259735/69‑2 (MQ=255)

                                                                ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg       <  1:4195173/69‑2 (MQ=255)

                                                                aTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCCATAGATTGCGGGGAGATCATCTTAc      >  1:5497410/1‑71 (MQ=255)

                                                                 tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                     >  1:3907274/1‑55 (MQ=255)

                                                                 tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg       <  1:5010779/69‑2 (MQ=255)

                                                                 tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc     <  1:1158933/71‑1 (MQ=255)

                                                                  cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGcc     <  1:941088/70‑1 (MQ=255)

                                                                   aGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCgg   >  1:1643765/1‑71 (MQ=255)

GCGTGGTGATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGT  >  minE/2562456‑2562594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`