BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,562,545	A→G	9.3%	V511A (GTA→GCA) ‡	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,562,545	0	A	G	9.3%	350.2 / 19.2	128	V511A (GTA→GCA) ‡	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base A (57/59); new base G (4/8); total (61/67)
Fisher's exact test for biased strand distribution p-value = 3.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTG  >  minE/2562475‑2562613

tataCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTa                                                                      >  1:2252248/1‑71 (MQ=255)

  taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTa                                                                      <  1:527234/69‑1 (MQ=255)

  taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAcc                                                                    >  1:286219/1‑71 (MQ=255)

  taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAc                                                                     >  1:3215675/1‑70 (MQ=255)

    cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                                   <  1:624641/70‑1 (MQ=255)

    cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                                   <  1:4714091/70‑1 (MQ=255)

      ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                                <  1:2667496/71‑1 (MQ=255)

      ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                                <  1:4217252/71‑1 (MQ=255)

      ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgcc                                                                 <  1:556458/70‑1 (MQ=255)

        tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                              >  1:4525704/1‑71 (MQ=255)

        tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                              <  1:756139/71‑1 (MQ=255)

        tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                              >  1:1357120/1‑71 (MQ=255)

        tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                              <  1:398186/71‑1 (MQ=255)

         gAAGATCAGGCTGTGTTTGACATTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                              >  1:4322132/1‑70 (MQ=255)

            gATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                           <  1:1825952/70‑1 (MQ=255)

             aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                                <  1:1260010/64‑1 (MQ=255)

             aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                           <  1:280348/69‑1 (MQ=255)

             aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                         <  1:1243591/71‑1 (MQ=255)

              tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                         >  1:4296573/1‑70 (MQ=255)

               cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                       >  1:3764316/1‑71 (MQ=255)

               cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                       >  1:772871/1‑71 (MQ=255)

               cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                       >  1:4599883/1‑71 (MQ=255)

               cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                       >  1:5518617/1‑71 (MQ=255)

                  gCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatag                                                    <  1:4509397/71‑1 (MQ=255)

                   cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                   <  1:3656059/71‑1 (MQ=255)

                   cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                   <  1:3044874/71‑1 (MQ=255)

                   cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                   <  1:1613557/71‑1 (MQ=255)

                      tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                      <  1:3451277/65‑1 (MQ=255)

                       gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                 >  1:1374445/1‑69 (MQ=255)

                       gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                 >  1:169429/1‑69 (MQ=255)

                       gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                        <  1:2046781/62‑1 (MQ=255)

                       gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                        <  1:1320160/62‑1 (MQ=255)

                       gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                        <  1:1202237/62‑1 (MQ=255)

                        tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                 >  1:3311967/1‑68 (MQ=255)

                        tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGcgatagcgatag                                              <  1:4068233/71‑1 (MQ=255)

                          tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                              >  1:1462011/1‑53 (MQ=255)

                          tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                              >  1:606529/1‑53 (MQ=255)

                          tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                            >  1:1335316/1‑71 (MQ=255)

                           gACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcaca                                                            >  1:5479547/1‑54 (MQ=255)

                           caCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                            >  1:4225911/2‑70 (MQ=255)

                            aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                                           <  1:2627415/70‑1 (MQ=255)

                            aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                                           <  1:581073/70‑1 (MQ=255)

                             cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGc                                         <  1:3321416/71‑1 (MQ=255)

                              aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                         >  1:1313452/1‑54 (MQ=255)

                              aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                         >  1:5369751/1‑54 (MQ=255)

                              aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                        <  1:1487587/71‑1 (MQ=255)

                              aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                        <  1:4966100/71‑1 (MQ=255)

                               gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                               <  1:678832/47‑1 (MQ=255)

                               gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                       <  1:93396/71‑1 (MQ=255)

                                tAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                               <  1:4692752/62‑1 (MQ=255)

                                   aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgata                                                     <  1:980012/53‑1 (MQ=255)

                                    gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCggg                                      >  1:296299/1‑67 (MQ=255)

                                    gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGgag                                   <  1:3349636/70‑1 (MQ=255)

                                    gCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                      >  1:156403/1‑67 (MQ=255)

                                      ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                 >  1:4808613/1‑70 (MQ=255)

                                      ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                 >  1:702624/1‑70 (MQ=255)

                                       gAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                               >  1:856635/1‑71 (MQ=255)

                                        aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcac                                                             <  1:4887408/40‑1 (MQ=255)

                                        aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                               <  1:518136/70‑1 (MQ=255)

                                         aCAAATCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                               <  1:3619352/37‑1 (MQ=255)

                                         aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                       >  1:4716623/1‑45 (MQ=255)

                                         aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                                   >  1:2561236/1‑65 (MQ=255)

                                         aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGAGATAGATTGCGGGgag                                   >  1:740332/1‑65 (MQ=255)

                                          cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                           >  1:2525723/1‑40 (MQ=255)

                                          cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                      <  1:3740631/61‑1 (MQ=255)

                                           agttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCtt                           >  1:267579/4‑71 (MQ=255)

                                           aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                               <  1:5156539/35‑1 (MQ=255)

                                           aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                               <  1:1256531/35‑1 (MQ=255)

                                           aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                               <  1:2488766/35‑1 (MQ=255)

                                            aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                          >  1:3274186/1‑71 (MQ=255)

                                             ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                                        <  1:1172219/55‑1 (MQ=255)

                                             ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                         <  1:3223874/70‑3 (MQ=255)

                                             ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg                          <  1:3259735/69‑2 (MQ=255)

                                             aTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCCATAGATTGCGGGGAGATCATCTTAc                         >  1:5497410/1‑71 (MQ=255)

                                              tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                                        >  1:3907274/1‑55 (MQ=255)

                                              tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc                        <  1:1158933/71‑1 (MQ=255)

                                               cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                         >  1:5118931/1‑67 (MQ=255)

                                               cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGcc                        <  1:941088/70‑1 (MQ=255)

                                                aGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCgg                      >  1:1643765/1‑71 (MQ=255)

                                                    tctTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                               <  1:2796059/42‑1 (MQ=255)

                                                    gctTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                                   >  1:1170062/2‑54 (MQ=255)

                                                      tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc                             >  1:4028601/1‑58 (MQ=255)

                                                      tttGCCCACCAGGCCTACCGC‑‑CACAGGCGATAGCGATAGATTGCGGGGAGatcatc                             >  1:1884747/1‑56 (MQ=255)

                                                      tttGCCCACCAGGACTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                >  1:4463872/1‑71 (MQ=255)

                                                        tGCCCACCAGCCCTACCGCCGCACAGgcgatagcga                                                 >  1:2539022/1‑36 (MQ=255)

                                                         gCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGccgc             <  1:4157060/71‑3 (MQ=255)

                                                         gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGa                   >  1:3194293/1‑65 (MQ=255)

                                                         gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc             >  1:5111582/1‑71 (MQ=255)

                                                         gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc             >  1:1941283/1‑71 (MQ=255)

                                                           ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg           >  1:3093636/1‑71 (MQ=255)

                                                            caccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcat                              <  1:1412731/51‑1 (MQ=255)

                                                                 ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                 >  1:5589699/1‑43 (MQ=255)

                                                                 ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                 >  1:15323/1‑43 (MQ=255)

                                                                 ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                 >  1:4750123/1‑43 (MQ=255)

                                                                 ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa     >  1:4464874/1‑71 (MQ=255)

                                                                    cTACCGCCGCACAGTCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg  >  1:3802392/1‑71 (MQ=255)

                                                                    cTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg  >  1:4143537/1‑71 (MQ=255)

                                                                     tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                           <  1:2330619/45‑1 (MQ=255)

                                                                     tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                            <  1:5234202/44‑1 (MQ=255)

                                                                     tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                            <  1:5077731/44‑1 (MQ=255)

TATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTG  >  minE/2562475‑2562613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`