BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,562,546	C→T	10.2%	V511I (GTA→ATA) ‡	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,562,546	0	C	T	10.2%	322.3 / 16.7	117	V511I (GTA→ATA) ‡	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base C (53/52); new base T (4/8); total (57/60)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGC  >  minE/2562477‑2562615

taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAcc                                                                      >  1:286219/1‑71 (MQ=255)

taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAcc                                                                      >  1:1960819/1‑71 (MQ=255)

taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAc                                                                       >  1:3215675/1‑70 (MQ=255)

  cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                                     <  1:624641/70‑1 (MQ=255)

  cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                                     <  1:4714091/70‑1 (MQ=255)

    ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                                  <  1:4217252/71‑1 (MQ=255)

    ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                                  <  1:2667496/71‑1 (MQ=255)

    ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgcc                                                                   <  1:556458/70‑1 (MQ=255)

      tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                >  1:4525704/1‑71 (MQ=255)

      tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                >  1:1357120/1‑71 (MQ=255)

      tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                <  1:756139/71‑1 (MQ=255)

      tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                <  1:398186/71‑1 (MQ=255)

       gAAGATCAGGCTGTGTTTGACATTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                >  1:4322132/1‑70 (MQ=255)

          gATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                             <  1:1825952/70‑1 (MQ=255)

           aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                                  <  1:1260010/64‑1 (MQ=255)

           aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                             <  1:280348/69‑1 (MQ=255)

           aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                           <  1:1243591/71‑1 (MQ=255)

            tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                           >  1:4296573/1‑70 (MQ=255)

             cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                         >  1:772871/1‑71 (MQ=255)

             cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                         >  1:3764316/1‑71 (MQ=255)

             cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                         >  1:4599883/1‑71 (MQ=255)

             cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                         >  1:5518617/1‑71 (MQ=255)

                gCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatag                                                      <  1:4509397/71‑1 (MQ=255)

                 cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                     <  1:3656059/71‑1 (MQ=255)

                 cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                     <  1:1613557/71‑1 (MQ=255)

                 cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                     <  1:3044874/71‑1 (MQ=255)

                    tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                        <  1:3451277/65‑1 (MQ=255)

                     gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                   >  1:1374445/1‑69 (MQ=255)

                     gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                          <  1:2046781/62‑1 (MQ=255)

                     gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                          <  1:1320160/62‑1 (MQ=255)

                     gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                          <  1:1202237/62‑1 (MQ=255)

                      tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                   >  1:3311967/1‑68 (MQ=255)

                      tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGcgatagcgatag                                                <  1:4068233/71‑1 (MQ=255)

                        tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                >  1:606529/1‑53 (MQ=255)

                        tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                >  1:1462011/1‑53 (MQ=255)

                        tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                              >  1:1335316/1‑71 (MQ=255)

                         gACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcaca                                                              >  1:5479547/1‑54 (MQ=255)

                         caCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                              >  1:4225911/2‑70 (MQ=255)

                          aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                                             <  1:2627415/70‑1 (MQ=255)

                          aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                                             <  1:581073/70‑1 (MQ=255)

                           cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGc                                           <  1:3321416/71‑1 (MQ=255)

                            aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                           >  1:1313452/1‑54 (MQ=255)

                            aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                           >  1:5369751/1‑54 (MQ=255)

                            aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                          <  1:4966100/71‑1 (MQ=255)

                            aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                          <  1:1487587/71‑1 (MQ=255)

                             gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                                 <  1:678832/47‑1 (MQ=255)

                             gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                         <  1:93396/71‑1 (MQ=255)

                              tAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                 <  1:4692752/62‑1 (MQ=255)

                                 aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgata                                                       <  1:980012/53‑1 (MQ=255)

                                  gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCggg                                        >  1:296299/1‑67 (MQ=255)

                                  gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGgag                                     <  1:3349636/70‑1 (MQ=255)

                                    ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                   >  1:702624/1‑70 (MQ=255)

                                    ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                   >  1:4808613/1‑70 (MQ=255)

                                      aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcac                                                               <  1:4887408/40‑1 (MQ=255)

                                      aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                         <  1:1567484/46‑1 (MQ=255)

                                      aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                                 <  1:518136/70‑1 (MQ=255)

                                       aCAAATCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                                 <  1:3619352/37‑1 (MQ=255)

                                       aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                         >  1:4716623/1‑45 (MQ=255)

                                       aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                                     >  1:2561236/1‑65 (MQ=255)

                                        cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                             >  1:2525723/1‑40 (MQ=255)

                                        cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                        <  1:3740631/61‑1 (MQ=255)

                                         agttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCtt                             >  1:267579/4‑71 (MQ=255)

                                         aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                                 <  1:5156539/35‑1 (MQ=255)

                                         aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                                 <  1:2488766/35‑1 (MQ=255)

                                         aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                                 <  1:1256531/35‑1 (MQ=255)

                                          aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                            >  1:753411/1‑71 (MQ=255)

                                          aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                            >  1:3274186/1‑71 (MQ=255)

                                           ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                                          <  1:1172219/55‑1 (MQ=255)

                                           ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                           <  1:3223874/70‑3 (MQ=255)

                                           ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg                            <  1:3259735/69‑2 (MQ=255)

                                           aTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCCATAGATTGCGGGGAGATCATCTTAc                           >  1:5497410/1‑71 (MQ=255)

                                            tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                                          >  1:3907274/1‑55 (MQ=255)

                                            tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc                          <  1:1158933/71‑1 (MQ=255)

                                             cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                           >  1:5118931/1‑67 (MQ=255)

                                             cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGcc                          <  1:941088/70‑1 (MQ=255)

                                              aGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCgg                        >  1:1643765/1‑71 (MQ=255)

                                                  tctTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                 <  1:2796059/42‑1 (MQ=255)

                                                  gctTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                                     >  1:1170062/2‑54 (MQ=255)

                                                    tttGCCCACCAGGCCTACCGCCGCACAGGcgatagcgatag                                                <  1:1825887/41‑1 (MQ=255)

                                                    tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc                               >  1:4028601/1‑58 (MQ=255)

                                                    tttGCCCACCAGGCCTACCGC‑‑CACAGGCGATAGCGATAGATTGCGGGGAGatcatc                               >  1:1884747/1‑56 (MQ=255)

                                                    tttGCCCACCAGGACTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                  >  1:4463872/1‑71 (MQ=255)

                                                      tGCCCACCAGCCCTACCGCCGCACAGgcgatagcga                                                   >  1:2539022/1‑36 (MQ=255)

                                                       gCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGccgc               <  1:4157060/71‑3 (MQ=255)

                                                       gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGa                     >  1:3194293/1‑65 (MQ=255)

                                                       gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc               >  1:5111582/1‑71 (MQ=255)

                                                         ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg             >  1:3093636/1‑71 (MQ=255)

                                                             caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg         <  1:1457428/71‑1 (MQ=255)

                                                               ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                   >  1:5589699/1‑43 (MQ=255)

                                                               ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                   >  1:4750123/1‑43 (MQ=255)

                                                               ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                   >  1:15323/1‑43 (MQ=255)

                                                               ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa       >  1:4464874/1‑71 (MQ=255)

                                                                  cTACCGCCGCACAGTCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg    >  1:3802392/1‑71 (MQ=255)

                                                                  cTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg    >  1:4143537/1‑71 (MQ=255)

                                                                   tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                             <  1:2330619/45‑1 (MQ=255)

                                                                   tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                              <  1:5234202/44‑1 (MQ=255)

                                                                   tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                              <  1:5077731/44‑1 (MQ=255)

                                                                     ccgccgCACAGGCGATAGCGATAGATTGCGGGgag                                     >  1:671121/1‑35 (MQ=255)

                                                                     ccgccgCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                              >  1:5424943/1‑42 (MQ=255)

                                                                     ccgccgCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggc  <  1:1358432/70‑1 (MQ=255)

TACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGC  >  minE/2562477‑2562615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`