BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,562,553	A→G	12.9%	C508C (TGT→TGC)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,562,553	0	A	G	12.9%	283.6 / 28.5	116	C508C (TGT→TGC)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base A (52/49); new base G (7/8); total (59/57)
Fisher's exact test for biased strand distribution p-value = 7.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.62e-01

Only 100 of 126 total aligned reads displayed.

TGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAC  >  minE/2562483‑2562621

tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                      >  1:4525704/1‑71 (MQ=255)

tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                      <  1:756139/71‑1 (MQ=255)

tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                      <  1:398186/71‑1 (MQ=255)

tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                      >  1:1357120/1‑71 (MQ=255)

    gATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                                   <  1:1825952/70‑1 (MQ=255)

     aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                                   <  1:280348/69‑1 (MQ=255)

     aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                                 <  1:1243591/71‑1 (MQ=255)

      tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                                 >  1:4296573/1‑70 (MQ=255)

       cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                               >  1:5518617/1‑71 (MQ=255)

       cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                               >  1:3764316/1‑71 (MQ=255)

       cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                               >  1:4599883/1‑71 (MQ=255)

       cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                               >  1:772871/1‑71 (MQ=255)

           cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                           <  1:4318108/71‑1 (MQ=255)

           cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                           <  1:3656059/71‑1 (MQ=255)

           cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                           <  1:1613557/71‑1 (MQ=255)

           cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                           <  1:3044874/71‑1 (MQ=255)

              tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgat                                                        <  1:1353602/71‑1 (MQ=255)

              tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                         <  1:5121931/70‑1 (MQ=255)

              tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                              <  1:3451277/65‑1 (MQ=255)

               gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                         >  1:1374445/1‑69 (MQ=255)

               gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                         >  1:169429/1‑69 (MQ=255)

               gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                                <  1:1320160/62‑1 (MQ=255)

               gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                                <  1:1202237/62‑1 (MQ=255)

                tttGACAGTAAAGCTGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                       <  1:2911125/70‑1 (MQ=255)

                tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGcgatagcgatag                                                      <  1:4068233/71‑1 (MQ=255)

                  tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                      >  1:606529/1‑53 (MQ=255)

                  tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                      >  1:1462011/1‑53 (MQ=255)

                   gACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcaca                                                                    >  1:5479547/1‑54 (MQ=255)

                   gACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                                    >  1:630133/1‑70 (MQ=255)

                   caCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                                    >  1:4225911/2‑70 (MQ=255)

                    aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                                                   <  1:2627415/70‑1 (MQ=255)

                     cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGc                                                 <  1:3321416/71‑1 (MQ=255)

                      aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                                 >  1:1313452/1‑54 (MQ=255)

                      aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                                 >  1:5369751/1‑54 (MQ=255)

                      aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                                <  1:1487587/71‑1 (MQ=255)

                      aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                                <  1:4966100/71‑1 (MQ=255)

                       gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                               <  1:93396/71‑1 (MQ=255)

                        tAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                       <  1:4692752/62‑1 (MQ=255)

                          aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgggg                                             <  1:1311113/70‑1 (MQ=255)

                           aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgata                                                             <  1:980012/53‑1 (MQ=255)

                           aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                              >  1:5267372/1‑68 (MQ=255)

                            gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGgag                                           <  1:3349636/70‑1 (MQ=255)

                              ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                         >  1:4808613/1‑70 (MQ=255)

                              ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                         >  1:2565244/1‑70 (MQ=255)

                              ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                         >  1:702624/1‑70 (MQ=255)

                               gAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                                       >  1:856635/1‑71 (MQ=255)

                                aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcac                                                                     <  1:4887408/40‑1 (MQ=255)

                                aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                               <  1:1567484/46‑1 (MQ=255)

                                aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                                       <  1:518136/70‑1 (MQ=255)

                                  cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                                   >  1:2525723/1‑40 (MQ=255)

                                  cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                              <  1:3740631/61‑1 (MQ=255)

                                   agttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCtt                                   >  1:267579/4‑71 (MQ=255)

                                    aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                                  >  1:3274186/1‑71 (MQ=255)

                                    aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                                  >  1:753411/1‑71 (MQ=255)

                                     ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                                                <  1:1172219/55‑1 (MQ=255)

                                     ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                                 <  1:3223874/70‑3 (MQ=255)

                                     ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg                                  <  1:3259735/69‑2 (MQ=255)

                                     aTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCCATAGATTGCGGGGAGATCATCTTAc                                 >  1:5497410/1‑71 (MQ=255)

                                      tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                                                >  1:3907274/1‑55 (MQ=255)

                                      tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg                                  <  1:5010779/69‑2 (MQ=255)

                                      tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc                                <  1:1158933/71‑1 (MQ=255)

                                       cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                                 >  1:5118931/1‑67 (MQ=255)

                                       cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGcc                                <  1:941088/70‑1 (MQ=255)

                                        aGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCgg                              >  1:1643765/1‑71 (MQ=255)

                                            gctTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                                           >  1:1170062/2‑54 (MQ=255)

                                              tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc                                     >  1:4028601/1‑58 (MQ=255)

                                              tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                        >  1:5659472/1‑71 (MQ=255)

                                              tttGCCCACCAGGCCTACCGC‑‑CACAGGCGATAGCGATAGATTGCGGGGAGatcatc                                     >  1:1884747/1‑56 (MQ=255)

                                              tttGCCCACCAGGACTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                        >  1:4463872/1‑71 (MQ=255)

                                                tGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                         >  1:4490880/1‑36 (MQ=255)

                                                tGCCCACCAGCCCTACCGCCGCACAGgcgatagcga                                                         >  1:2539022/1‑36 (MQ=255)

                                                 gCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGccgc                     <  1:4157060/71‑3 (MQ=255)

                                                 gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                     >  1:1941283/1‑71 (MQ=255)

                                                 gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                     >  1:5111582/1‑71 (MQ=255)

                                                  cccACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                     <  1:2776999/70‑1 (MQ=255)

                                                   ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg                   >  1:3093636/1‑71 (MQ=255)

                                                      ccaCGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                   <  1:4749875/52‑1 (MQ=255)

                                                       caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg               <  1:1457428/71‑1 (MQ=255)

                                                        aGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg               >  1:3101389/1‑70 (MQ=255)

                                                         ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                         >  1:4750123/1‑43 (MQ=255)

                                                         ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                         >  1:15323/1‑43 (MQ=255)

                                                         ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                         >  1:5589699/1‑43 (MQ=255)

                                                         ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa             >  1:4464874/1‑71 (MQ=255)

                                                            cTACCGCCGCACAGTCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg          >  1:3802392/1‑71 (MQ=255)

                                                            cTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg          >  1:4143537/1‑71 (MQ=255)

                                                             tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                   <  1:334080/45‑1 (MQ=255)

                                                             tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                   <  1:4616808/45‑1 (MQ=255)

                                                             tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                   <  1:2330619/45‑1 (MQ=255)

                                                              gtcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgc        >  1:2997310/3‑71 (MQ=255)

                                                              gtcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgc        >  1:4290080/3‑71 (MQ=255)

                                                               tcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgct       >  1:2850094/2‑70 (MQ=255)

                                                               ccgccgCACAGGCGATAGCGATAGATTGCGGGgag                                           >  1:671121/1‑35 (MQ=255)

                                                               ccgccgCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                                    >  1:5424943/1‑42 (MQ=255)

                                                               ccgccgCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggc        <  1:1358432/70‑1 (MQ=255)

                                                                cgccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTgg         <  1:4372768/68‑1 (MQ=255)

                                                                 gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAc                                 >  1:1967843/1‑43 (MQ=255)

                                                                 gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAc                                 >  1:3246385/1‑43 (MQ=255)

                                                                 gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt                             <  1:2815257/47‑1 (MQ=255)

                                                                    gcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc  <  1:38867/71‑1 (MQ=255)

                                                                      acaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc  >  1:4993910/1‑69 (MQ=255)

TGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAC  >  minE/2562483‑2562621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`