BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,562,595	G→T	6.5%	V494V (GTC→GTA)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,562,595	0	G	T	6.5%	349.3 / 5.9	107	V494V (GTC→GTA)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base G (43/57); new base T (4/3); total (47/60)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 107 total aligned reads displayed.

TTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGC  >  minE/2562529‑2562663

tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                                                                  >  1:5659472/1‑71 (MQ=255)

tttGCCCACCAGGACTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                                                                  >  1:4463872/1‑71 (MQ=255)

   gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGa                                                                     >  1:3194293/1‑65 (MQ=255)

   gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                                                               >  1:1941283/1‑71 (MQ=255)

   gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                                                               >  1:5111582/1‑71 (MQ=255)

    cccACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                                                               <  1:2776999/70‑1 (MQ=255)

     ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg                                                             >  1:3093636/1‑71 (MQ=255)

         caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg                                                         <  1:1457428/71‑1 (MQ=255)

          aGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg                                                         >  1:3101389/1‑70 (MQ=255)

           ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                                                       >  1:4464874/1‑71 (MQ=255)

              cTACCGCCGCACAGTCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg                                                    >  1:3802392/1‑71 (MQ=255)

              cTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg                                                    >  1:4143537/1‑71 (MQ=255)

                gtcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgc                                                  >  1:4290080/3‑71 (MQ=255)

                gtcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgc                                                  >  1:2997310/3‑71 (MQ=255)

                 tcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgct                                                 >  1:2850094/2‑70 (MQ=255)

                 ccgccgCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggc                                                  <  1:1358432/70‑1 (MQ=255)

                  cgccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTgg                                                   <  1:4372768/68‑1 (MQ=255)

                   gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggcgg                                                >  1:4962567/1‑70 (MQ=255)

                      gcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc                                            <  1:905535/71‑1 (MQ=255)

                      gcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc                                            <  1:38867/71‑1 (MQ=255)

                        acaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc                                            >  1:4993910/1‑69 (MQ=255)

                         caGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                                                       >  1:3867121/1‑57 (MQ=255)

                         caGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                                                       >  1:1403883/1‑57 (MQ=255)

                          aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc                                        <  1:4477575/71‑1 (MQ=255)

                          aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc                                        <  1:5458937/71‑1 (MQ=255)

                           ggCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggt                                                           >  1:1532170/1‑51 (MQ=255)

                           ggCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc                                        >  1:1784924/1‑70 (MQ=255)

                            gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc                                        <  1:3603062/69‑1 (MQ=255)

                            gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                      <  1:3190675/71‑1 (MQ=255)

                            gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                      <  1:4956448/71‑1 (MQ=255)

                            gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                      >  1:3698900/1‑71 (MQ=255)

                            gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                      >  1:3692022/1‑71 (MQ=255)

                            gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                      >  1:3198310/1‑71 (MQ=255)

                              gatagcgatagATTGCGGGGAGATCATCTTACCGGTGa                                                                     <  1:4402374/38‑1 (MQ=255)

                              gatagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                      >  1:3006952/1‑69 (MQ=255)

                               atagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACAggtggt                                                        <  1:4902000/50‑1 (MQ=255)

                                tagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                      >  1:760940/1‑67 (MQ=255)

                                tagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGa                                  <  1:933193/71‑1 (MQ=255)

                                tagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGa                                  <  1:4985427/71‑1 (MQ=255)

                                cggcgatagATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTTGCTGCCAc                                            >  1:3575025/3‑61 (MQ=255)

                                 agcgatagATTGCGGGGAGATCATCTTACCGGTGa                                                                     >  1:322529/1‑35 (MQ=255)

                                 agcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtg                                                          >  1:4864617/1‑46 (MQ=255)

                                 agcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtg                                                          >  1:884592/1‑46 (MQ=255)

                                 agcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                      >  1:1040689/1‑66 (MQ=255)

                                 agcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                      >  1:2991359/1‑66 (MQ=255)

                                  gcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGAt                                 <  1:3589432/70‑1 (MQ=255)

                                  gcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGAt                                 <  1:3799621/70‑1 (MQ=255)

                                   cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATc                                <  1:4468703/70‑1 (MQ=255)

                                   cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                               <  1:732248/71‑1 (MQ=255)

                                   cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                               <  1:3975860/71‑1 (MQ=255)

                                   cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                               <  1:2206542/71‑1 (MQ=255)

                                   cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                               <  1:2402859/71‑1 (MQ=255)

                                     atagATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCgctggtgt                                                      <  1:5347731/46‑3 (MQ=255)

                                     atagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCaga                             <  1:4903605/71‑1 (MQ=255)

                                     atagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                               <  1:2099888/69‑1 (MQ=255)

                                     atagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                               <  1:4125777/69‑1 (MQ=255)

                                      tagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                               >  1:3856092/1‑68 (MQ=255)

                                       agATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCagag                            >  1:5168142/1‑70 (MQ=255)

                                       agATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAgaga                           >  1:3199942/1‑71 (MQ=255)

                                        gATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAgaga                           >  1:1835337/1‑70 (MQ=255)

                                        gATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAgaga                           >  1:4528684/1‑70 (MQ=255)

                                         aTTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTTGCTGCCACCAGCa                                       <  1:4719050/57‑1 (MQ=255)

                                         aTTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                               <  1:4274733/65‑1 (MQ=255)

                                         aTTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                               <  1:466454/65‑1 (MQ=255)

                                         aTTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACg                         >  1:2290931/1‑71 (MQ=255)

                                            gCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGcc                       <  1:4432399/70‑1 (MQ=255)

                                                ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt                  <  1:2518998/71‑1 (MQ=255)

                                                ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt                  <  1:1706535/71‑1 (MQ=255)

                                                ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt                  <  1:5347637/71‑1 (MQ=255)

                                                ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt                  <  1:2742185/71‑1 (MQ=255)

                                                ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACAAGCAACAGATCAGAGACGCCAAttt                  <  1:4530105/71‑1 (MQ=255)

                                                 gagaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttg                 <  1:5386954/71‑1 (MQ=255)

                                                 gagaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttg                 <  1:5653578/71‑1 (MQ=255)

                                                 gagaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttg                 <  1:725154/71‑1 (MQ=255)

                                                  agaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgt                <  1:1250462/71‑1 (MQ=255)

                                                  agaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgt                <  1:3876918/71‑1 (MQ=255)

                                                    atcatcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGcc                       >  1:1369123/1‑62 (MQ=255)

                                                    atcatcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGc                        <  1:5144430/61‑1 (MQ=255)

                                                     tcatcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACg                         <  1:1601814/59‑1 (MQ=255)

                                                     tcatcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACg                         <  1:2444955/59‑1 (MQ=255)

                                                      catcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg              <  1:2369473/69‑1 (MQ=255)

                                                      catcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTtgttgt             >  1:5348102/1‑70 (MQ=255)

                                                      catcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTtgtg            >  1:1459403/1‑71 (MQ=255)

                                                        tcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt                  <  1:5438080/63‑1 (MQ=255)

                                                         cTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg              >  1:4879143/1‑66 (MQ=255)

                                                          ttACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgctgc        >  1:411249/1‑71 (MQ=255)

                                                           tACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttg                 >  1:3090777/1‑61 (MQ=255)

                                                            aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgctgc        <  1:514004/69‑1 (MQ=255)

                                                            aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgctgc        <  1:2011742/69‑1 (MQ=255)

                                                            aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgct          <  1:2268682/67‑1 (MQ=255)

                                                            aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgct          <  1:2085545/67‑1 (MQ=255)

                                                            aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCgg      >  1:1921218/1‑71 (MQ=255)

                                                            aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCg       <  1:2193849/70‑1 (MQ=255)

                                                              cGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg              <  1:4908609/61‑1 (MQ=255)

                                                              cGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg              <  1:2623471/61‑1 (MQ=255)

                                                              cGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg              <  1:4991421/61‑1 (MQ=255)

                                                              cGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg              <  1:1003998/61‑1 (MQ=255)

                                                              cGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTg    <  1:4458571/71‑1 (MQ=255)

                                                                gTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCag                              <  1:1497158/43‑1 (MQ=255)

                                                                gTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGc  <  1:1204056/71‑1 (MQ=255)

TTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGC  >  minE/2562529‑2562663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`