BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,562,733	G→A	17.0%	F448F (TTC→TTT)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,562,733	0	G	A	17.0%	219.2 / 31.1	94	F448F (TTC→TTT)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base G (33/45); new base A (11/5); total (44/50)
Fisher's exact test for biased strand distribution p-value = 6.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGG  >  minE/2562666‑2562803

gCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGa                                                                       <  1:915637/69‑1 (MQ=255)

 cAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAAc                                                                     <  1:4768465/70‑1 (MQ=255)

 cAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCAGTCAGGAATACCCCCAGCCAGCCGAGGAAc                                                                     <  1:2596432/70‑1 (MQ=255)

  aGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACgg                                                                   >  1:383742/1‑71 (MQ=255)

      cggcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCg                                                                 <  1:5575591/69‑1 (MQ=255)

        gcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaa                                                             <  1:2752940/71‑1 (MQ=255)

          gAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaaga                                                           <  1:699560/71‑1 (MQ=255)

           aaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa                                                          <  1:1829411/71‑1 (MQ=255)

           aaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa                                                          <  1:1830777/71‑1 (MQ=255)

            aCAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGaagaag                                                         >  1:3731184/1‑71 (MQ=255)

            aCAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGaagaag                                                         >  1:170310/1‑71 (MQ=255)

              aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                                                       <  1:1533219/71‑1 (MQ=255)

              aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                                                       <  1:4194809/71‑1 (MQ=255)

              aGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACgg                                                                   >  1:4234613/1‑59 (MQ=255)

              aGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa                                                          >  1:1320443/1‑68 (MQ=255)

               gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                                                      <  1:3965777/71‑1 (MQ=255)

               gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                                                      <  1:260290/71‑1 (MQ=255)

               gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCACCAGCCAGCCGAGGAACGGCGagaaga                                                           <  1:4815674/66‑1 (MQ=255)

                 gCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                                                    >  1:4260842/1‑71 (MQ=255)

                 gCGTTAGAGGAGGTGTCCGAGCCGCTAAGCAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                                                    >  1:3230097/1‑71 (MQ=255)

                    ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaag                                                            <  1:5207988/60‑1 (MQ=255)

                    ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt                                                   <  1:5457942/69‑1 (MQ=255)

                    ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt                                                   <  1:3331589/69‑1 (MQ=255)

                    ttAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaacat                                                 <  1:4839846/71‑4 (MQ=255)

                      agagGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACggt                                                                  >  1:4210752/1‑51 (MQ=19)

                      aGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCAt                                               >  1:3162993/1‑71 (MQ=255)

                         tGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGa                                             <  1:1856228/70‑1 (MQ=255)

                          gAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGAcc                                           >  1:2169980/1‑71 (MQ=255)

                           aGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                                                       <  1:3951219/58‑1 (MQ=255)

                            ggTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGagaa                                                             >  1:5360147/1‑51 (MQ=19)

                                tCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGTGagaag                                                            >  1:3513565/1‑48 (MQ=255)

                                tCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaag                                                            >  1:140899/1‑48 (MQ=255)

                                  cGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaac                                                   >  1:1739630/1‑54 (MQ=255)

                                  cGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcg                                   >  1:4110773/1‑71 (MQ=255)

                                   gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                  <  1:2511757/71‑1 (MQ=255)

                                   gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                  <  1:2298358/71‑1 (MQ=255)

                                   gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                  <  1:4141274/71‑1 (MQ=255)

                                    aCCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                 <  1:3007749/71‑1 (MQ=255)

                                     cccGGTCAGGAATACCCCCAGCCAGACGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                <  1:4808242/71‑1 (MQ=255)

                                         gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATg                                                  <  1:274018/49‑1 (MQ=255)

                                         gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                <  1:2223517/67‑1 (MQ=255)

                                         gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                <  1:2022168/67‑1 (MQ=255)

                                         gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                            <  1:813294/71‑1 (MQ=255)

                                           cAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                              >  1:5265785/1‑67 (MQ=255)

                                           cAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                              >  1:4962244/1‑67 (MQ=255)

                                           cAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                              >  1:2502727/1‑67 (MQ=255)

                                           aaGGAATACGCCCAGCCAGCCGAGAAACGGTGagaaga                                                           >  1:5273705/2‑38 (MQ=21)

                                            aGGAATACGCCCAGCCAGCCGAGAAACGGTGagaaga                                                           >  1:4663760/1‑37 (MQ=25)

                                            aGGAATACGCCCAGCCAGCCGAGAAACGGTGagaaga                                                           >  1:3549661/1‑37 (MQ=25)

                                            aGGAATACGCCCAGCCAGCCGAGAAACGGTGagaaga                                                           >  1:3669812/1‑37 (MQ=25)

                                             ggAATACGCCCAGCCAGCCGAGAAACGGTGagaag                                                            <  1:3911580/35‑1 (MQ=21)

                                             ggAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCAAGTGCCAgc                        >  1:820881/1‑71 (MQ=255)

                                              gAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                 <  1:912513/61‑1 (MQ=255)

                                               aaTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                <  1:1851296/61‑1 (MQ=255)

                                               aaTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                            <  1:813052/65‑1 (MQ=255)

                                               aaTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                            <  1:4916532/65‑1 (MQ=255)

                                               aaTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                            <  1:4579725/65‑1 (MQ=255)

                                                aTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGAc                                            >  1:5112987/1‑48 (MQ=255)

                                                aTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGAc                                            >  1:1599010/1‑48 (MQ=255)

                                                aTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                     <  1:306401/71‑1 (MQ=255)

                                                 tACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                                                    <  1:802741/39‑1 (MQ=16)

                                                 tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                                                      <  1:1308663/37‑1 (MQ=255)

                                                 tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                  <  1:923332/57‑1 (MQ=255)

                                                 tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                  <  1:578560/57‑1 (MQ=255)

                                                 tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcgc                      >  1:1001235/1‑69 (MQ=255)

                                                 tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcgc                      >  1:5560770/1‑69 (MQ=255)

                                                   cccccAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                >  1:5599825/1‑57 (MQ=255)

                                                    ccccAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcgc                      <  1:1302933/66‑1 (MQ=255)

                                                      ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCa                                                >  1:106915/1‑38 (MQ=255)

                                                      ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCa                                                >  1:1229053/1‑38 (MQ=255)

                                                      ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAg                               >  1:1079552/1‑55 (MQ=255)

                                                      ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                              >  1:555489/1‑56 (MQ=255)

                                                      ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTg                             >  1:1862425/1‑57 (MQ=255)

                                                      ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt               <  1:2558007/71‑1 (MQ=255)

                                                      ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt               <  1:5148250/71‑1 (MQ=255)

                                                       cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                <  1:2182636/53‑1 (MQ=255)

                                                       cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt               >  1:4552243/1‑70 (MQ=255)

                                                       cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt               >  1:5220691/1‑70 (MQ=255)

                                                       cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt               >  1:4914321/1‑70 (MQ=255)

                                                         gccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat            <  1:3079144/71‑1 (MQ=255)

                                                          ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt               <  1:3007529/67‑1 (MQ=255)

                                                          ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat            >  1:5356351/1‑70 (MQ=255)

                                                          ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat            >  1:3048281/1‑70 (MQ=255)

                                                          ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat            >  1:3694618/1‑70 (MQ=255)

                                                           cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga          >  1:3207927/1‑71 (MQ=255)

                                                           cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga          <  1:3350138/71‑1 (MQ=255)

                                                           cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatg           <  1:5118957/70‑1 (MQ=255)

                                                           cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatg           <  1:1558803/70‑1 (MQ=255)

                                                            agccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAg                   <  1:4301423/61‑1 (MQ=255)

                                                             gccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga          >  1:2091311/1‑69 (MQ=255)

                                                              ccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                            <  1:1453931/50‑1 (MQ=255)

                                                               cGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGt      >  1:1914376/1‑71 (MQ=255)

                                                                gAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTc     >  1:4456498/1‑71 (MQ=255)

                                                                  ggAACGGCGAGAAGAAGCTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAgtg                 >  1:3937947/1‑57 (MQ=255)

                                                                   gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                 <  1:1177604/40‑1 (MQ=255)

                                                                   gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                 <  1:822002/40‑1 (MQ=255)

                                                                   gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                 <  1:5023028/40‑1 (MQ=255)

                                                                   gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCGAGTGTTGATGACAGTCCg   >  1:1519842/1‑70 (MQ=255)

                                                                   gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCgg  <  1:5551658/71‑1 (MQ=255)

                                                                   gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGGGCGcc                                 <  1:4455938/40‑1 (MQ=255)

GCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGG  >  minE/2562666‑2562803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`