| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,772 | A→G | 15.9% | I367I (ATT→ATC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,772 | 0 | A | G | 15.9% | 277.8 / 33.9 | 107 | I367I (ATT→ATC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (42/48); new base G (10/7); total (52/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
ATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCA > minE/311704‑311842 | aTCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAAt > 1:2996679/1‑71 (MQ=255) tCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATc < 1:2303522/71‑1 (MQ=255) agaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCa < 1:208985/69‑1 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACg < 1:2329970/71‑1 (MQ=255) gaAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACg < 1:113161/71‑1 (MQ=255) aaGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAAc > 1:2228641/1‑69 (MQ=255) aaGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGt > 1:2290489/1‑71 (MQ=255) aaGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAAAACCACCGGTACGGCAATGGTCGGAATCaa > 1:2715058/1‑68 (MQ=255) cAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgcg > 1:2071402/1‑71 (MQ=255) aGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgcgcg > 1:4846186/1‑70 (MQ=255) aGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCgg < 1:3663923/71‑1 (MQ=255) ggcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGa < 1:2655864/71‑1 (MQ=255) gcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCACCGTCGCGCgg > 1:3497152/1‑69 (MQ=255) cAAGGACGGCAAAGGGCCCGAGCAATACCACCGGTACGGCAATGGTCGGa < 1:4347077/50‑1 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt < 1:1388741/71‑1 (MQ=255) aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg > 1:2930044/1‑71 (MQ=255) aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg > 1:2082518/1‑71 (MQ=255) aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGGCGGAATCAACGTCGCGCGGAAGTTCTg < 1:1104062/71‑1 (MQ=255) aGGACGGCAAAGGTCCCGAGAAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg > 1:3748501/1‑71 (MQ=255) aCGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAg > 1:612465/1‑71 (MQ=255) ggCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGa < 1:3631523/71‑1 (MQ=255) cAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc < 1:5176462/71‑1 (MQ=255) aaGGTCCCCAGCAGCACCACCGGTACGGCGATGGTCGGGATCAGCGTGGCGCGGAAGTTCTGCAGGAACAg > 1:5237249/1‑71 (MQ=11) aaGGTCCCCAGCAGCACCACCGGTACGGCGATGGTCGGGATCAGCGTGGCGCGGAAGTTCTGCAGGAACAg > 1:2637157/1‑71 (MQ=11) aaGGTCCCCAGCAGCACCACCGGTACGGCGATGGTCGGGATCAGCGTGGCGCGGAAGTTCTGCAGGAACAg > 1:5215938/1‑71 (MQ=11) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:2961535/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:4473877/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:4773430/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:2566969/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:2496179/56‑1 (MQ=255) ggTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGa > 1:1236659/1‑70 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:4441074/1‑50 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa < 1:122985/64‑1 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACa < 1:3948426/70‑1 (MQ=255) cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAcatacata < 1:128063/71‑1 (MQ=255) cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa < 1:2566942/71‑1 (MQ=255) aacAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:4922309/3‑71 (MQ=2) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:2609659/1‑59 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:2780819/1‑59 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:2727410/1‑59 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc < 1:2970212/71‑1 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc < 1:966593/71‑1 (MQ=255) gCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAcc < 1:1008852/71‑1 (MQ=255) acAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:1661494/2‑70 (MQ=2) acAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:1975921/2‑70 (MQ=2) aacaccacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAg > 1:4596896/4‑71 (MQ=2) aaTACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:511256/65‑1 (MQ=255) accacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAgg < 1:1106224/69‑1 (MQ=9) accaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:822193/1‑41 (MQ=255) accaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:2786853/1‑41 (MQ=255) accaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:295208/1‑41 (MQ=255) ccaccGGTACGGCAATGGTCGGATTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:4568543/1‑70 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:1083101/1‑40 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:1165736/1‑40 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt > 1:4393492/1‑61 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:4377344/1‑70 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAac < 1:403968/71‑1 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGAAGGAACAg > 1:2907685/1‑55 (MQ=255) caccGGTACGGCAATGGTCGGAATCAACGTCGCGCgg > 1:5433506/1‑37 (MQ=255) caccGGTACGGCAATGGTCGGAATCAACGTCGCGCgg > 1:4014668/1‑37 (MQ=255) caccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc < 1:5398554/52‑1 (MQ=255) caccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc < 1:3971975/52‑1 (MQ=255) acgggTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAg > 1:3124164/4‑65 (MQ=2) acgggTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAacac < 1:5493422/68‑1 (MQ=9) accGGTACGGCAATGGTCGGAATCAACTTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac < 1:4566776/71‑1 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:4882378/66‑1 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac < 1:3584368/71‑1 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac < 1:297754/71‑1 (MQ=255) accGGCACCGCGATCGTCGGGATCAGCGTCGCCCGCCAATTCTGCAGGAACAGGAACATCACCAGGAagac < 1:4785712/71‑1 (MQ=2) ccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAg > 1:266931/1‑52 (MQ=255) ccGGTACGGCAATGGTAGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa > 1:4796944/1‑51 (MQ=255) ggTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg > 1:1448485/1‑71 (MQ=255) aCGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgat > 1:4248622/1‑71 (MQ=255) ccgCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGcat < 1:3775086/68‑1 (MQ=9) ccgCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGcat < 1:786931/68‑1 (MQ=9) cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:109107/71‑1 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc > 1:1191143/1‑42 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatgat < 1:1848565/71‑1 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatgat < 1:5089969/71‑1 (MQ=255) cAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAcc < 1:1768456/53‑1 (MQ=2) aaTGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGACCACCAGcataatg > 1:5560352/1‑69 (MQ=2) aaTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa > 1:2928644/1‑50 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGa > 1:1807280/1‑42 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:1821335/47‑1 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:1387417/47‑1 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa < 1:2646836/48‑1 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACg < 1:2180989/60‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:1701577/1‑37 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:1423131/53‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:1524122/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:5267957/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:3730336/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:3283076/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:3154050/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:2015780/61‑1 (MQ=255) ggTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg > 1:4168476/1‑60 (MQ=255) ggTCGGAATCAACGTCGCGAGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCtt > 1:5645169/1‑71 (MQ=255) gTCGGATTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:4376172/59‑1 (MQ=255) cGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGa > 1:1014996/1‑71 (MQ=255) aaTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCa < 1:5031656/71‑1 (MQ=255) | ATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCA > minE/311704‑311842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |