BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,562,748	G→C	5.5%	T443T (ACC→ACG) ‡	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,562,748	0	G	C	5.5%	282.2 / 4.5	91	T443T (ACC→ACG) ‡	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base G (34/52); new base C (3/2); total (37/54)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 103 total aligned reads displayed.

ACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAT  >  minE/2562678‑2562816

aCAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGaagaag                                                                      >  1:170310/1‑71 (MQ=255)

aCAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGaagaag                                                                      >  1:3731184/1‑71 (MQ=255)

  aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                                                                    <  1:4194809/71‑1 (MQ=255)

  aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                                                                    <  1:1533219/71‑1 (MQ=255)

   gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                                                                   <  1:260290/71‑1 (MQ=255)

   gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                                                                   <  1:3965777/71‑1 (MQ=255)

     gCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                                                                 >  1:4260842/1‑71 (MQ=255)

     gCGTTAGAGGAGGTGTCCGAGCCGCTAAGCAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                                                                 >  1:3230097/1‑71 (MQ=255)

        ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt                                                                <  1:3331589/69‑1 (MQ=255)

        ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt                                                                <  1:5457942/69‑1 (MQ=255)

        ttAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaacat                                                              <  1:4839846/71‑4 (MQ=255)

          aGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCAt                                                            >  1:3162993/1‑71 (MQ=255)

             tGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGa                                                          <  1:1856228/70‑1 (MQ=255)

              gAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGAcc                                                        >  1:2169980/1‑71 (MQ=255)

               aGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                                                                    <  1:3951219/58‑1 (MQ=255)

                      cGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaac                                                                >  1:1739630/1‑54 (MQ=255)

                      cGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcg                                                >  1:4110773/1‑71 (MQ=255)

                       gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                               <  1:2511757/71‑1 (MQ=255)

                       gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                               <  1:2298358/71‑1 (MQ=255)

                       gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                               <  1:4141274/71‑1 (MQ=255)

                        aCCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                              <  1:3007749/71‑1 (MQ=255)

                         cccGGTCAGGAATACCCCCAGCCAGACGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                             <  1:4808242/71‑1 (MQ=255)

                             gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                             <  1:2022168/67‑1 (MQ=255)

                             gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                             <  1:2223517/67‑1 (MQ=255)

                             gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                                         <  1:813294/71‑1 (MQ=255)

                               cAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                                           >  1:4962244/1‑67 (MQ=255)

                               cAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                                           >  1:5265785/1‑67 (MQ=255)

                               cAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                                           >  1:2502727/1‑67 (MQ=255)

                                 ggAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCAAGTGCCAgc                                     >  1:820881/1‑71 (MQ=255)

                                  gAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                              <  1:912513/61‑1 (MQ=255)

                                   aaTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                             <  1:1851296/61‑1 (MQ=255)

                                   aaTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                                         <  1:4579725/65‑1 (MQ=255)

                                   aaTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                                         <  1:4916532/65‑1 (MQ=255)

                                   aaTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                                         <  1:813052/65‑1 (MQ=255)

                                    aTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGAc                                                         >  1:1599010/1‑48 (MQ=255)

                                    aTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGAc                                                         >  1:5112987/1‑48 (MQ=255)

                                    aTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  <  1:306401/71‑1 (MQ=255)

                                     tACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                                                                 <  1:802741/39‑1 (MQ=16)

                                     tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                                                                   <  1:1308663/37‑1 (MQ=255)

                                     tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                               <  1:578560/57‑1 (MQ=255)

                                     tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                               <  1:923332/57‑1 (MQ=255)

                                     tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcgc                                   >  1:1001235/1‑69 (MQ=255)

                                     tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcgc                                   >  1:5560770/1‑69 (MQ=255)

                                       cccccAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                             >  1:5599825/1‑57 (MQ=255)

                                        ccccAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcgc                                   <  1:1302933/66‑1 (MQ=255)

                                          ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCa                                                             >  1:1229053/1‑38 (MQ=255)

                                          ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCa                                                             >  1:106915/1‑38 (MQ=255)

                                          ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAg                                            >  1:1079552/1‑55 (MQ=255)

                                          ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                                           >  1:555489/1‑56 (MQ=255)

                                          ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTg                                          >  1:1862425/1‑57 (MQ=255)

                                          ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                            <  1:5148250/71‑1 (MQ=255)

                                          ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                            <  1:2558007/71‑1 (MQ=255)

                                           cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                             <  1:2182636/53‑1 (MQ=255)

                                           cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                            >  1:5220691/1‑70 (MQ=255)

                                           cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                            >  1:4552243/1‑70 (MQ=255)

                                           cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                            >  1:4914321/1‑70 (MQ=255)

                                             gccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                         <  1:3079144/71‑1 (MQ=255)

                                              ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                            <  1:3007529/67‑1 (MQ=255)

                                              ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                         >  1:3694618/1‑70 (MQ=255)

                                              ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                         >  1:5356351/1‑70 (MQ=255)

                                              ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                         >  1:3048281/1‑70 (MQ=255)

                                               cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga                       >  1:3207927/1‑71 (MQ=255)

                                               cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga                       <  1:3350138/71‑1 (MQ=255)

                                               cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatg                        <  1:1558803/70‑1 (MQ=255)

                                               cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatg                        <  1:5118957/70‑1 (MQ=255)

                                                agccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAg                                <  1:4301423/61‑1 (MQ=255)

                                                 gccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga                       >  1:2091311/1‑69 (MQ=255)

                                                  ccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                                         <  1:1453931/50‑1 (MQ=255)

                                                   cGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGt                   >  1:1914376/1‑71 (MQ=255)

                                                    gAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTc                  >  1:4456498/1‑71 (MQ=255)

                                                      ggAACGGCGAGAAGAAGCTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAgtg                              >  1:3937947/1‑57 (MQ=255)

                                                       gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                              <  1:5023028/40‑1 (MQ=255)

                                                       gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                              <  1:1177604/40‑1 (MQ=255)

                                                       gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                              <  1:822002/40‑1 (MQ=255)

                                                       gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCGAGTGTTGATGACAGTCCg                >  1:1519842/1‑70 (MQ=255)

                                                       gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCgg               <  1:5551658/71‑1 (MQ=255)

                                                       gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGGGCGcc                                              <  1:4455938/40‑1 (MQ=255)

                                                        aaCGGCGAGAAGAAGGTTAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  <  1:4233128/51‑1 (MQ=255)

                                                        aaCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  <  1:5502785/51‑1 (MQ=255)

                                                        aaCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  <  1:2677076/51‑1 (MQ=255)

                                                        aaCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  <  1:2886405/51‑1 (MQ=255)

                                                         aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa             <  1:1619930/71‑1 (MQ=255)

                                                         aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa             <  1:1743839/71‑1 (MQ=255)

                                                         aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa             >  1:3912729/1‑71 (MQ=255)

                                                          cGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAg                                >  1:1157908/1‑51 (MQ=255)

                                                          cGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAg                                >  1:1150994/1‑51 (MQ=255)

                                                          cGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAAt            <  1:4564681/71‑1 (MQ=255)

                                                             cGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  <  1:2796350/46‑1 (MQ=255)

                                                              gagaAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGtt        >  1:5157284/1‑71 (MQ=255)

                                                               agaagaAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTc       <  1:3103375/71‑1 (MQ=255)

                                                                 aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  >  1:4786743/1‑42 (MQ=255)

                                                                 aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  >  1:525615/1‑42 (MQ=255)

                                                                 aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTcc                 <  1:618363/59‑1 (MQ=255)

                                                                 aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa     <  1:1586393/71‑1 (MQ=255)

                                                                  agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg      >  1:4599888/1‑69 (MQ=255)

                                                                  agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg      >  1:5578002/1‑69 (MQ=255)

                                                                  agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa     >  1:4212015/1‑70 (MQ=255)

                                                                   gaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa   >  1:290423/1‑71 (MQ=255)

                                                                   gaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa   >  1:3084253/1‑71 (MQ=255)

                                                                     agGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAt  <  1:5508246/70‑1 (MQ=255)

ACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAT  >  minE/2562678‑2562816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`