BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,562,750	T→G	5.0%	T443P (ACC→CCC) ‡	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,562,750	0	T	G	5.0%	265.4 / 5.0	99	T443P (ACC→CCC) ‡	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base T (41/53); new base G (3/2); total (44/55)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 103 total aligned reads displayed.

AGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAT  >  minE/2562680‑2562816

aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                                                                    <  1:1533219/71‑1 (MQ=255)

aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                                                                    <  1:4194809/71‑1 (MQ=255)

 gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                                                                   <  1:260290/71‑1 (MQ=255)

 gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                                                                   <  1:3965777/71‑1 (MQ=255)

   gCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                                                                 >  1:4260842/1‑71 (MQ=255)

   gCGTTAGAGGAGGTGTCCGAGCCGCTAAGCAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                                                                 >  1:3230097/1‑71 (MQ=255)

      ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt                                                                <  1:3331589/69‑1 (MQ=255)

      ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt                                                                <  1:5457942/69‑1 (MQ=255)

      ttAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaacat                                                              <  1:4839846/71‑4 (MQ=255)

        aGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCAt                                                            >  1:3162993/1‑71 (MQ=255)

           tGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGa                                                          <  1:1856228/70‑1 (MQ=255)

            gAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGAcc                                                        >  1:2169980/1‑71 (MQ=255)

             aGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt                                                                    <  1:3951219/58‑1 (MQ=255)

                    cGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaac                                                                >  1:1739630/1‑54 (MQ=255)

                    cGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcg                                                >  1:4110773/1‑71 (MQ=255)

                     gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                               <  1:4141274/71‑1 (MQ=255)

                     gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                               <  1:2511757/71‑1 (MQ=255)

                     gACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                               <  1:2298358/71‑1 (MQ=255)

                      aCCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                              <  1:3007749/71‑1 (MQ=255)

                       cccGGTCAGGAATACCCCCAGCCAGACGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                             <  1:4808242/71‑1 (MQ=255)

                           gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATg                                                               <  1:274018/49‑1 (MQ=255)

                           gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                             <  1:2223517/67‑1 (MQ=255)

                           gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                             <  1:2022168/67‑1 (MQ=255)

                           gTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                                         <  1:813294/71‑1 (MQ=255)

                             cAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                                           >  1:4962244/1‑67 (MQ=255)

                             cAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                                           >  1:2502727/1‑67 (MQ=255)

                             cAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                                           >  1:5265785/1‑67 (MQ=255)

                               ggAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCAAGTGCCAgc                                     >  1:820881/1‑71 (MQ=255)

                                gAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                              <  1:912513/61‑1 (MQ=255)

                                 aaTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                             <  1:1851296/61‑1 (MQ=255)

                                 aaTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                                         <  1:4579725/65‑1 (MQ=255)

                                 aaTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                                         <  1:813052/65‑1 (MQ=255)

                                 aaTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                                         <  1:4916532/65‑1 (MQ=255)

                                  aTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGAc                                                         >  1:1599010/1‑48 (MQ=255)

                                  aTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGAc                                                         >  1:5112987/1‑48 (MQ=255)

                                  aTACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  <  1:306401/71‑1 (MQ=255)

                                   tACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa                                                                 <  1:802741/39‑1 (MQ=16)

                                   tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg                                                                   <  1:1308663/37‑1 (MQ=255)

                                   tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                               <  1:923332/57‑1 (MQ=255)

                                   tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTgcgc                                               <  1:578560/57‑1 (MQ=255)

                                   tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcgc                                   >  1:1001235/1‑69 (MQ=255)

                                   tACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcgc                                   >  1:5560770/1‑69 (MQ=255)

                                     cccccAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                             >  1:5599825/1‑57 (MQ=255)

                                      ccccAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAgcgc                                   <  1:1302933/66‑1 (MQ=255)

                                        ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCa                                                             >  1:106915/1‑38 (MQ=255)

                                        ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCa                                                             >  1:1229053/1‑38 (MQ=255)

                                        ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAg                                            >  1:1079552/1‑55 (MQ=255)

                                        ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGt                                           >  1:555489/1‑56 (MQ=255)

                                        ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTg                                          >  1:1862425/1‑57 (MQ=255)

                                        ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                            <  1:2558007/71‑1 (MQ=255)

                                        ccagccagCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                            <  1:5148250/71‑1 (MQ=255)

                                         cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCa                                             <  1:2182636/53‑1 (MQ=255)

                                         cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                            >  1:5220691/1‑70 (MQ=255)

                                         cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                            >  1:4552243/1‑70 (MQ=255)

                                         cagccagcCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                            >  1:4914321/1‑70 (MQ=255)

                                           gccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                         <  1:3079144/71‑1 (MQ=255)

                                            ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGtt                            <  1:3007529/67‑1 (MQ=255)

                                            ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                         >  1:3048281/1‑70 (MQ=255)

                                            ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                         >  1:5356351/1‑70 (MQ=255)

                                            ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                         >  1:3694618/1‑70 (MQ=255)

                                             cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga                       <  1:3350138/71‑1 (MQ=255)

                                             cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga                       >  1:3207927/1‑71 (MQ=255)

                                             cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatg                        <  1:1558803/70‑1 (MQ=255)

                                             cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatg                        <  1:5118957/70‑1 (MQ=255)

                                              agccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAg                                <  1:4301423/61‑1 (MQ=255)

                                               gccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga                       >  1:2091311/1‑69 (MQ=255)

                                                ccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGc                                         <  1:1453931/50‑1 (MQ=255)

                                                 cGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGt                   >  1:1914376/1‑71 (MQ=255)

                                                  gAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTc                  >  1:4456498/1‑71 (MQ=255)

                                                    ggAACGGCGAGAAGAAGCTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAgtg                              >  1:3937947/1‑57 (MQ=255)

                                                     gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                              <  1:822002/40‑1 (MQ=255)

                                                     gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                              <  1:1177604/40‑1 (MQ=255)

                                                     gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGcc                                              <  1:5023028/40‑1 (MQ=255)

                                                     gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCGAGTGTTGATGACAGTCCg                >  1:1519842/1‑70 (MQ=255)

                                                     gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCgg               <  1:5551658/71‑1 (MQ=255)

                                                     gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGGGCGcc                                              <  1:4455938/40‑1 (MQ=255)

                                                      aaCGGCGAGAAGAAGGTTAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  <  1:4233128/51‑1 (MQ=255)

                                                      aaCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  <  1:4593652/51‑1 (MQ=255)

                                                      aaCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  <  1:5502785/51‑1 (MQ=255)

                                                      aaCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  <  1:2677076/51‑1 (MQ=255)

                                                      aaCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  <  1:2886405/51‑1 (MQ=255)

                                                       aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa             <  1:1743839/71‑1 (MQ=255)

                                                       aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa             <  1:1619930/71‑1 (MQ=255)

                                                       aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa             >  1:3912729/1‑71 (MQ=255)

                                                        cGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAg                                >  1:1150994/1‑51 (MQ=255)

                                                        cGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAg                                >  1:1157908/1‑51 (MQ=255)

                                                        cGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAAt            <  1:4564681/71‑1 (MQ=255)

                                                            gagaAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGtt        >  1:5157284/1‑71 (MQ=255)

                                                             agaagaAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTc       <  1:3103375/71‑1 (MQ=255)

                                                               aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  >  1:525615/1‑42 (MQ=255)

                                                               aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGcc                                  >  1:4786743/1‑42 (MQ=255)

                                                               aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTcc                 <  1:618363/59‑1 (MQ=255)

                                                               aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa     <  1:1586393/71‑1 (MQ=255)

                                                                agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg      >  1:5578002/1‑69 (MQ=255)

                                                                agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa     >  1:4212015/1‑70 (MQ=255)

                                                                 gaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa   >  1:3084253/1‑71 (MQ=255)

                                                                 gaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa   >  1:290423/1‑71 (MQ=255)

                                                                   agGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAt  <  1:5508246/70‑1 (MQ=255)

                                                                     gTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAgtgt                             <  1:3709797/41‑1 (MQ=255)

                                                                      tGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                         >  1:5239583/1‑44 (MQ=255)

AGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAT  >  minE/2562680‑2562816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`