BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,562,792	A→T	8.0%	S429T (TCA→ACA) ‡	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,562,792	0	A	T	8.0%	279.2 / 13.3	100	S429T (TCA→ACA) ‡	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base A (48/44); new base T (3/5); total (51/49)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

Only 100 of 121 total aligned reads displayed.

GCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGC  >  minE/2562723‑2562861

gccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                                                                      <  1:3079144/71‑1 (MQ=255)

 ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                                                                      >  1:5356351/1‑70 (MQ=255)

 ccagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgat                                                                      >  1:3048281/1‑70 (MQ=255)

  cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga                                                                    <  1:3350138/71‑1 (MQ=255)

  cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatga                                                                    >  1:3207927/1‑71 (MQ=255)

  cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatg                                                                     <  1:5118957/70‑1 (MQ=255)

  cagccGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTtgatg                                                                     <  1:1558803/70‑1 (MQ=255)

      cGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGt                                                                >  1:1914376/1‑71 (MQ=255)

          gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCGAGTGTTGATGACAGTCCg                                                             >  1:1519842/1‑70 (MQ=255)

          gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCgg                                                            <  1:5551658/71‑1 (MQ=255)

            aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa                                                          >  1:3912729/1‑71 (MQ=255)

            aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa                                                          <  1:1743839/71‑1 (MQ=255)

            aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa                                                          <  1:1619930/71‑1 (MQ=255)

             cGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAAt                                                         <  1:4564681/71‑1 (MQ=255)

                 gagaAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGtt                                                     >  1:5157284/1‑71 (MQ=255)

                  agaagaAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTc                                                    <  1:3103375/71‑1 (MQ=255)

                     agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg                                                   >  1:4599888/1‑69 (MQ=255)

                     agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg                                                   >  1:5578002/1‑69 (MQ=255)

                     agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa                                                  >  1:4212015/1‑70 (MQ=255)

                      gaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                                >  1:290423/1‑71 (MQ=255)

                        agGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAt                                               <  1:5508246/70‑1 (MQ=255)

                              aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                        <  1:4212770/71‑1 (MQ=255)

                              aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                        <  1:2597269/71‑1 (MQ=255)

                              aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                        <  1:2842602/71‑1 (MQ=255)

                              aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                        <  1:3844628/71‑1 (MQ=255)

                              aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                        <  1:1409973/71‑1 (MQ=255)

                              aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                        <  1:5047878/71‑1 (MQ=255)

                              aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                        <  1:5415613/71‑1 (MQ=255)

                              aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                        <  1:835765/71‑1 (MQ=255)

                                gCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATaa                                             <  1:2481695/64‑1 (MQ=255)

                                 cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                     >  1:3893123/1‑71 (MQ=255)

                                 cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                     >  1:5169307/1‑71 (MQ=255)

                                 cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                     >  1:106148/1‑71 (MQ=255)

                                 cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                     >  1:1106407/1‑71 (MQ=255)

                                 cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCAAAATAAAGGCGAAt                                     >  1:638628/1‑71 (MQ=255)

                                     aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaa                                                 >  1:593341/1‑55 (MQ=255)

                                     aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaa                                                 >  1:5344643/1‑55 (MQ=255)

                                     aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAg                                           >  1:3647968/1‑61 (MQ=255)

                                      ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAg                                >  1:1020275/1‑71 (MQ=255)

                                       cGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                              <  1:4622033/56‑1 (MQ=255)

                                       cGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                              <  1:5176153/56‑1 (MQ=255)

                                          tgtgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg                                                   <  1:1141503/48‑1 (MQ=255)

                                            tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa                                                  <  1:5610371/47‑1 (MQ=255)

                                            tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa                                                  <  1:1259051/47‑1 (MQ=255)

                                            tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATaaa                                            <  1:4947671/53‑1 (MQ=255)

                                              cgcCAGTGCCAGCGCCAGTGTTGATGCCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc                        >  1:4262622/1‑71 (MQ=255)

                                              cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                                >  1:4698748/1‑47 (MQ=255)

                                              cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                                >  1:1418383/1‑47 (MQ=255)

                                              cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                                >  1:1587077/1‑47 (MQ=255)

                                              cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAt                                               <  1:5028110/48‑1 (MQ=255)

                                                ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAcc                       >  1:3398620/1‑70 (MQ=255)

                                                ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAcc                       >  1:4946521/1‑70 (MQ=255)

                                                ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCg                      <  1:4700014/71‑1 (MQ=255)

                                                 cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCa                           <  1:2633657/65‑1 (MQ=255)

                                                 cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGccaa                     <  1:5280274/71‑3 (MQ=255)

                                                  aGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                    >  1:2159907/1‑71 (MQ=255)

                                                  aGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                    >  1:239227/1‑71 (MQ=255)

                                                   gTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATg                   <  1:1936672/71‑1 (MQ=255)

                                                    tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                              <  1:4429701/43‑1 (MQ=255)

                                                    tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                              <  1:1021423/43‑1 (MQ=255)

                                                    tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                              <  1:3836005/43‑1 (MQ=255)

                                                    tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAgg                                          <  1:4159643/47‑1 (MQ=255)

                                                    tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAgg                                          <  1:3974195/47‑1 (MQ=255)

                                                    tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAT‑AAGGCGAATGCCAGCACCATACCGATGGa                 <  1:1114507/71‑1 (MQ=255)

                                                     accagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                 >  1:4741748/2‑71 (MQ=255)

                                                      ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                 >  1:769714/1‑70 (MQ=255)

                                                      ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                 <  1:408708/70‑1 (MQ=255)

                                                      ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                 >  1:118170/1‑70 (MQ=255)

                                                      ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                >  1:5545325/1‑71 (MQ=255)

                                                      ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                <  1:3234846/71‑1 (MQ=255)

                                                       cAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                >  1:2693699/1‑70 (MQ=255)

                                                       cAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                >  1:5599599/1‑70 (MQ=255)

                                                       cAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                 <  1:2241158/69‑1 (MQ=255)

                                                       cAGCGACAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATCCCGATGGAg                >  1:2008963/1‑70 (MQ=255)

                                                        aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                              <  1:1635298/39‑1 (MQ=255)

                                                          cgcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                >  1:4506506/1‑67 (MQ=255)

                                                           gcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc                        <  1:4140878/58‑1 (MQ=255)

                                                            ccAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa              <  1:5275838/67‑1 (MQ=255)

                                                              aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCa                           >  1:1596421/1‑52 (MQ=255)

                                                              aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCa                           >  1:3025819/1‑52 (MQ=255)

                                                              aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg        <  1:3210737/71‑1 (MQ=255)

                                                               gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                      >  1:2072745/1‑40 (MQ=255)

                                                               gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                      >  1:5662538/1‑40 (MQ=255)

                                                               gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                      >  1:1405628/1‑40 (MQ=255)

                                                               gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGa                                       <  1:2713171/39‑1 (MQ=255)

                                                               gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg        >  1:4043806/1‑70 (MQ=255)

                                                               gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg        >  1:248751/1‑70 (MQ=255)

                                                                tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                    <  1:464769/57‑1 (MQ=255)

                                                                tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa      <  1:5484630/71‑1 (MQ=255)

                                                                tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa      <  1:1048842/71‑1 (MQ=255)

                                                                 gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCgtt                    <  1:33239/56‑3 (MQ=255)

                                                                  ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATgg                  >  1:4503950/1‑57 (MQ=255)

                                                                   tgatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCaga    <  1:2691275/70‑1 (MQ=255)

                                                                    gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa                                      >  1:4942369/1‑35 (MQ=255)

                                                                    gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa                                      >  1:4512518/1‑35 (MQ=255)

                                                                    gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa                                      >  1:1249381/1‑35 (MQ=255)

                                                                    gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa                                      >  1:4021223/1‑35 (MQ=255)

                                                                    gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc  >  1:3373119/1‑71 (MQ=255)

                                                                    gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc  >  1:5530277/1‑71 (MQ=255)

                                                                    gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc  >  1:741882/1‑71 (MQ=255)

GCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGC  >  minE/2562723‑2562861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`