| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,562,811 | C→G | 5.3% | S422S (TCG→TCC) ‡ | lldP ← | L‑lactate permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,562,811 | 0 | C | G | 5.3% | 472.9 / 10.4 | 149 | S422S (TCG→TCC) ‡ | lldP | L‑lactate permease |
| Reads supporting (aligned to +/- strand): ref base C (78/63); new base G (3/5); total (81/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01 | |||||||||||
Only 100 of 153 total aligned reads displayed.
AGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCT > minE/2562741‑2562879 | agaagaAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTc < 1:3103375/71‑1 (MQ=255) aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa < 1:1586393/71‑1 (MQ=255) agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg > 1:4599888/1‑69 (MQ=255) agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg > 1:5578002/1‑69 (MQ=255) agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa > 1:4212015/1‑70 (MQ=255) gaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa > 1:290423/1‑71 (MQ=255) agGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAt < 1:5508246/70‑1 (MQ=255) aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg < 1:1409973/71‑1 (MQ=255) aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg < 1:2597269/71‑1 (MQ=255) aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg < 1:4212770/71‑1 (MQ=255) aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg < 1:2822310/71‑1 (MQ=255) aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg < 1:5047878/71‑1 (MQ=255) aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg < 1:2842602/71‑1 (MQ=255) aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg < 1:835765/71‑1 (MQ=255) gCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATaa < 1:2481695/64‑1 (MQ=255) cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt > 1:106148/1‑71 (MQ=255) cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt > 1:5169307/1‑71 (MQ=255) cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt > 1:1106407/1‑71 (MQ=255) cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt > 1:3893123/1‑71 (MQ=255) cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCAAAATAAAGGCGAAt > 1:638628/1‑71 (MQ=255) aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaa > 1:5344643/1‑55 (MQ=255) aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAg > 1:3647968/1‑61 (MQ=255) aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGc > 1:4808018/1‑69 (MQ=255) ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGc > 1:5466577/1‑62 (MQ=255) ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAg > 1:1020275/1‑71 (MQ=255) cGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa < 1:4622033/56‑1 (MQ=255) cGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa < 1:5176153/56‑1 (MQ=255) tgtgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg < 1:1141503/48‑1 (MQ=255) tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa < 1:5610371/47‑1 (MQ=255) tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATaaa < 1:4947671/53‑1 (MQ=255) cgcCAGTGCCAGCGCCAGTGTTGATGCCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc > 1:4262622/1‑71 (MQ=255) cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa > 1:4698748/1‑47 (MQ=255) cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAt < 1:5028110/48‑1 (MQ=255) cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc > 1:5556386/1‑71 (MQ=255) ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAcc > 1:4946521/1‑70 (MQ=255) ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCg < 1:4700014/71‑1 (MQ=255) cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCa < 1:2633657/65‑1 (MQ=255) cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGccaa < 1:5280274/71‑3 (MQ=255) gTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATg < 1:1936672/71‑1 (MQ=255) tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa < 1:4429701/43‑1 (MQ=255) tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa < 1:3836005/43‑1 (MQ=255) tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa < 1:1021423/43‑1 (MQ=255) tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAgg < 1:4159643/47‑1 (MQ=255) tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAT‑AAGGCGAATGCCAGCACCATACCGATGGa < 1:1114507/71‑1 (MQ=255) ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa < 1:408708/70‑1 (MQ=255) ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa > 1:769714/1‑70 (MQ=255) ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATa > 1:1750329/1‑62 (MQ=255) ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg < 1:3234846/71‑1 (MQ=255) cAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg > 1:2693699/1‑70 (MQ=255) cAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa < 1:2241158/69‑1 (MQ=255) cAGCGACAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATCCCGATGGAg > 1:2008963/1‑70 (MQ=255) gcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa > 1:4009300/1‑70 (MQ=255) ccAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa < 1:5275838/67‑1 (MQ=255) aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCa > 1:3025819/1‑52 (MQ=255) aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCa > 1:1596421/1‑52 (MQ=255) gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa > 1:2072745/1‑40 (MQ=255) gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa > 1:1405628/1‑40 (MQ=255) gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa > 1:5662538/1‑40 (MQ=255) gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg > 1:248751/1‑70 (MQ=255) gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCgtt < 1:33239/56‑3 (MQ=255) ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATgg > 1:4503950/1‑57 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa > 1:1249381/1‑35 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa > 1:4512518/1‑35 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa > 1:4021223/1‑35 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc > 1:741882/1‑71 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc > 1:3373119/1‑71 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc > 1:5530277/1‑71 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc > 1:1380088/1‑71 (MQ=255) gatgaCAGT‑CGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc > 1:3243766/1‑70 (MQ=255) gaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCAt > 1:3298977/1‑44 (MQ=255) gTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa > 1:1311283/1‑54 (MQ=255) gTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa > 1:1273389/1‑54 (MQ=255) gTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCACACCGATGGAGTAGa > 1:5237428/1‑54 (MQ=255) tCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAg < 1:1224405/52‑1 (MQ=255) cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCagag > 1:895726/1‑60 (MQ=255) cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAg > 1:4927924/1‑64 (MQ=255) cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCttt > 1:3395007/1‑70 (MQ=255) cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCttt > 1:1495555/1‑70 (MQ=255) gAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCg < 1:1667538/39‑1 (MQ=255) gAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAg < 1:4092279/71‑1 (MQ=255) aaTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGc > 1:98151/1‑71 (MQ=255) aTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGa > 1:3855829/1‑38 (MQ=255) aTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCg > 1:4474416/1‑71 (MQ=255) tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGa < 1:4163940/37‑1 (MQ=255) tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt < 1:2100207/38‑1 (MQ=255) tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt < 1:5232841/38‑1 (MQ=255) tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt < 1:3068388/38‑1 (MQ=255) tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt < 1:1822402/38‑1 (MQ=255) tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg > 1:300886/1‑50 (MQ=255) tAGTTCGAAATAAACGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGc < 1:5394239/69‑1 (MQ=255) aGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa < 1:3090354/51‑1 (MQ=255) aGTTCGAAAT‑AAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa < 1:1365776/50‑1 (MQ=255) gTTCGAAATAAAGGCGAATGCCAGCACCATACCGATg > 1:636022/1‑37 (MQ=255) gTTCGAAATAAAGGCGAATGCCAGCACCATACCGATg > 1:3503937/1‑37 (MQ=255) gTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtgc < 1:230483/71‑1 (MQ=255) gTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtgc > 1:856961/1‑71 (MQ=255) ttCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGtt < 1:89909/58‑1 (MQ=255) ttCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtgct < 1:2344279/71‑1 (MQ=255) ttCGAAATAAAGGCGAATGCCAGCACCATACCGAAGGAGTAGATGGGCAGAGCCAGtt < 1:1658827/58‑1 (MQ=255) cGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa < 1:2279147/39‑1 (MQ=255) | AGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCT > minE/2562741‑2562879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |