BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,562,816	T→C	5.6%	I421V (ATT→GTT) ‡	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,562,816	0	T	C	5.6%	419.3 / 9.6	142	I421V (ATT→GTT) ‡	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base T (69/65); new base C (3/5); total (72/70)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 144 total aligned reads displayed.

AGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGCCGA  >  minE/2562747‑2562884

      aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                                               <  1:2597269/71‑1 (MQ=255)

      aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                                               <  1:835765/71‑1 (MQ=255)

      aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                                               <  1:2822310/71‑1 (MQ=255)

      aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                                               <  1:1409973/71‑1 (MQ=255)

      aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                                               <  1:5047878/71‑1 (MQ=255)

        gCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATaa                                                                    <  1:2481695/64‑1 (MQ=255)

         cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                                            >  1:106148/1‑71 (MQ=255)

         cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                                            >  1:3893123/1‑71 (MQ=255)

         cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                                            >  1:1106407/1‑71 (MQ=255)

         cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCAAAATAAAGGCGAAt                                                            >  1:638628/1‑71 (MQ=255)

             aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAg                                                                  >  1:3647968/1‑61 (MQ=255)

             aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGc                                                          >  1:4808018/1‑69 (MQ=255)

              ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGc                                                                >  1:5466577/1‑62 (MQ=255)

              ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAg                                                       <  1:4606298/71‑1 (MQ=255)

              ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAg                                                       >  1:1020275/1‑71 (MQ=255)

               cGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                                     <  1:5176153/56‑1 (MQ=255)

                    tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATaaa                                                                   <  1:4947671/53‑1 (MQ=255)

                      cgcCAGTGCCAGCGCCAGTGTTGATGCCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc                                               >  1:4262622/1‑71 (MQ=255)

                      cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAt                                                                      <  1:5028110/48‑1 (MQ=255)

                        ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAcc                                              >  1:4946521/1‑70 (MQ=255)

                        ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAcc                                              >  1:3398620/1‑70 (MQ=255)

                        ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCg                                             <  1:4700014/71‑1 (MQ=255)

                         cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCa                                                  <  1:2633657/65‑1 (MQ=255)

                         cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGccaa                                            <  1:5280274/71‑3 (MQ=255)

                         cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGccaa                                            <  1:4206624/71‑3 (MQ=255)

                            tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                                     <  1:3836005/43‑1 (MQ=255)

                            tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                                     <  1:1021423/43‑1 (MQ=255)

                            tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATg                                          >  1:5103044/1‑70 (MQ=255)

                            tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAT‑AAGGCGAATGCCAGCACCATACCGATGGa                                        <  1:1114507/71‑1 (MQ=255)

                              ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                                        >  1:118170/1‑70 (MQ=255)

                              ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                                        <  1:408708/70‑1 (MQ=255)

                              ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                                       >  1:5545325/1‑71 (MQ=255)

                              ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                                       <  1:3234846/71‑1 (MQ=255)

                               cAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                                       >  1:5599599/1‑70 (MQ=255)

                               cAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                                        <  1:2241158/69‑1 (MQ=255)

                               cAGCGACAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATCCCGATGGAg                                       >  1:2008963/1‑70 (MQ=255)

                                aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                                     <  1:1635298/39‑1 (MQ=255)

                                  cgcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                                       >  1:4506506/1‑67 (MQ=255)

                                   gcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc                                               <  1:4140878/58‑1 (MQ=255)

                                   gcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa                                   >  1:4009300/1‑70 (MQ=255)

                                      aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCa                                                  >  1:3025819/1‑52 (MQ=255)

                                      aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCa                                                  >  1:1596421/1‑52 (MQ=255)

                                      aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg                               <  1:3210737/71‑1 (MQ=255)

                                       gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                                             >  1:1405628/1‑40 (MQ=255)

                                       gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                                             >  1:5662538/1‑40 (MQ=255)

                                       gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGa                                                              <  1:2713171/39‑1 (MQ=255)

                                       gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg                               >  1:248751/1‑70 (MQ=255)

                                        tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                                           <  1:464769/57‑1 (MQ=255)

                                        tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa                             <  1:1048842/71‑1 (MQ=255)

                                        tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa                             <  1:5484630/71‑1 (MQ=255)

                                           tgatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCaga                           <  1:2691275/70‑1 (MQ=255)

                                            gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa                                                             >  1:4512518/1‑35 (MQ=255)

                                            gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa                                                             >  1:1249381/1‑35 (MQ=255)

                                            gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa                                                             >  1:4021223/1‑35 (MQ=255)

                                            gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCagag                          <  1:892243/70‑1 (MQ=255)

                                            gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc                         >  1:741882/1‑71 (MQ=255)

                                            gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc                         >  1:5530277/1‑71 (MQ=255)

                                            gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc                         >  1:3373119/1‑71 (MQ=255)

                                            gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc                         >  1:1380088/1‑71 (MQ=255)

                                            gatgaCAGT‑CGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc                         >  1:3243766/1‑70 (MQ=255)

                                               gaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCAt                                                 >  1:3298977/1‑44 (MQ=255)

                                                aCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAg                      <  1:5286804/70‑1 (MQ=255)

                                                 cAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCag                            >  1:1691005/1‑63 (MQ=255)

                                                   gTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa                                   >  1:1311283/1‑54 (MQ=255)

                                                   gTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa                                   >  1:1273389/1‑54 (MQ=255)

                                                   gTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCACACCGATGGAGTAGa                                   >  1:5237428/1‑54 (MQ=255)

                                                    tCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCATTTCtt                 >  1:5191361/1‑71 (MQ=255)

                                                      cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCagag                          >  1:895726/1‑60 (MQ=255)

                                                      cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAg                      >  1:4927924/1‑64 (MQ=255)

                                                      cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCttt                >  1:4434644/1‑70 (MQ=255)

                                                      cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCttt                >  1:5252222/1‑70 (MQ=255)

                                                      cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCttt                <  1:2064984/70‑1 (MQ=255)

                                                      cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGAT‑‑GCAGAGCCAGTTCTTTCAg             >  1:3351507/1‑71 (MQ=255)

                                                       ggAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGt                                      <  1:3769596/47‑1 (MQ=255)

                                                        gAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCg                                             <  1:1667538/39‑1 (MQ=255)

                                                        gAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAg             <  1:4092279/71‑1 (MQ=255)

                                                        gAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAg             <  1:920468/71‑1 (MQ=255)

                                                         aaTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGc            >  1:818935/1‑71 (MQ=255)

                                                         aaTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGc            >  1:98151/1‑71 (MQ=255)

                                                          aTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGa                                            >  1:3855829/1‑38 (MQ=255)

                                                          aTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCg           >  1:4474416/1‑71 (MQ=255)

                                                           tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGa                                            <  1:4163940/37‑1 (MQ=255)

                                                           tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                                           <  1:2100207/38‑1 (MQ=255)

                                                           tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                                           <  1:3068388/38‑1 (MQ=255)

                                                           tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                                           <  1:1822402/38‑1 (MQ=255)

                                                           tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg                               >  1:735929/1‑50 (MQ=255)

                                                           tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg                               >  1:300886/1‑50 (MQ=255)

                                                           tAGTTCGAAATAAACGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGc            <  1:5394239/69‑1 (MQ=255)

                                                            aGTTCGAAAT‑AAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa                             <  1:1365776/50‑1 (MQ=255)

                                                             gTTCGAAATAAAGGCGAATGCCAGCACCATACCGATg                                          >  1:3503937/1‑37 (MQ=255)

                                                             gTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtgc        <  1:230483/71‑1 (MQ=255)

                                                              ttCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtgct       <  1:1887200/71‑1 (MQ=255)

                                                              ttCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtgct       <  1:2344279/71‑1 (MQ=255)

                                                                cGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                                     <  1:2279147/39‑1 (MQ=255)

                                                                cGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCa                       >  1:4261842/1‑53 (MQ=255)

                                                                cGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtg         >  1:2850442/1‑67 (MQ=255)

                                                                 gAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGcc    <  1:2080646/71‑1 (MQ=255)

                                                                  aaaTAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGt                     <  1:2167776/53‑1 (MQ=255)

                                                                   aaTAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGCCGa  <  1:1685378/71‑1 (MQ=255)

                                                                   aaTAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGCCGa  <  1:3264120/71‑1 (MQ=255)

AGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGCCGA  >  minE/2562747‑2562884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`