| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,075 | T→G | 5.2% | T40T (ACA→ACC) | mtlA ← | fused mannitol‑specific PTS enzyme IIABC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,075 | 0 | T | G | 5.2% | 204.3 / 4.0 | 77 | T40T (ACA→ACC) | mtlA | fused mannitol‑specific PTS enzyme IIABC components |
| Reads supporting (aligned to +/- strand): ref base T (37/36); new base G (2/2); total (39/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
CAGCAGCGGCAGGAGATAAGTGATCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTG > minE/2569006‑2569144 | cagcagCGGCAGGAGATAAGTGATCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTg > 1:4559175/1‑71 (MQ=255) gcagcGGCAGGAGATAAGTGATCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGtt < 1:3973611/71‑1 (MQ=255) gCAGGAGATAAGTGTTCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGaat > 1:5135964/1‑70 (MQ=255) cAGGAGATAAGTGATCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGaataa < 1:3059095/71‑1 (MQ=255) cAGGAGATAAGTGATCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGa > 1:3751525/1‑67 (MQ=255) aGGAGATAAGTGATCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGaataaa > 1:2261688/1‑71 (MQ=255) ggAGATAAGTGATCATCTGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGaataaat < 1:4093894/71‑1 (MQ=255) gagaTAAGTGATCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGaataaata < 1:776289/71‑1 (MQ=255) gaTAAGTGATCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAAc < 1:5445046/71‑1 (MQ=255) aTAAGTGATCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAAcg > 1:1841671/1‑71 (MQ=255) tAAGTGATCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGaataaat < 1:4488618/66‑1 (MQ=255) aaGTGATCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAataaataa > 1:174590/1‑67 (MQ=255) gATCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtga > 1:5623203/1‑71 (MQ=255) atcatcGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTg < 1:940132/52‑1 (MQ=255) atcatcGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTg < 1:2120624/52‑1 (MQ=255) atcatcGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtgat > 1:2336609/1‑71 (MQ=255) tcatcGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtgatg < 1:3854699/71‑1 (MQ=255) atcGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtgat > 1:3869856/1‑68 (MQ=255) tcGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGaataaat < 1:544750/56‑1 (MQ=255) tcGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGaataaat < 1:2686657/56‑1 (MQ=255) tcGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtgatga < 1:2582751/69‑1 (MQ=255) tcGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATa < 1:2056632/71‑1 (MQ=255) cGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGaataaat < 1:5166023/55‑1 (MQ=255) gACCAGCTTCGCGAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtgat < 1:4067389/60‑1 (MQ=255) gACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtgat < 1:2157232/60‑1 (MQ=255) gACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtgat < 1:5670246/60‑1 (MQ=255) aCCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGaataa > 1:1691802/1‑46 (MQ=255) aCCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAAcg > 1:316555/1‑52 (MQ=255) aCCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCAcgc < 1:3845802/71‑1 (MQ=255) aCCAGCTTCCCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCAcgc < 1:1166793/71‑1 (MQ=255) ccAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCAcgc > 1:4679329/1‑70 (MQ=255) cAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtga > 1:4446481/1‑56 (MQ=255) cAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGa > 1:67731/1‑71 (MQ=255) aaGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtga > 1:70154/2‑56 (MQ=255) aGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGAt < 1:5698130/71‑1 (MQ=255) cTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGaataaat < 1:1243928/43‑1 (MQ=255) cTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGaataaat < 1:4327753/43‑1 (MQ=255) cTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGaat < 1:5641014/39‑1 (MQ=255) cTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtgatga > 1:2670525/1‑56 (MQ=255) cTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATa > 1:2675980/1‑70 (MQ=255) ttCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATAccc > 1:4633505/1‑60 (MQ=255) ttCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATAccc > 1:648603/1‑60 (MQ=255) ttCGACAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAAcg > 1:2311176/1‑46 (MQ=255) tCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAataaataa > 1:2835131/1‑43 (MQ=255) cGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAAcg > 1:2617703/1‑71 (MQ=255) cGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAAAAAATAACGCTGTGATGATACGCCACGCGATa < 1:29603/67‑1 (MQ=255) gCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAAcgc < 1:2425969/71‑1 (MQ=255) aGCGTCTCGTTCGTTAACCACCCTGTTGGAATAAATAACGCGGtgat < 1:1450676/47‑1 (MQ=255) aGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGcc > 1:4570591/1‑71 (MQ=255) aGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGcc > 1:5613050/1‑71 (MQ=255) tctcGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGata < 1:5634626/71‑1 (MQ=255) ttcGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAata > 1:2891923/2‑70 (MQ=255) tcGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtgatga > 1:2236692/1‑43 (MQ=255) tcGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtgatga > 1:3661003/1‑43 (MQ=255) tcGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtgatg > 1:4925902/1‑42 (MQ=255) tcGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtgatg > 1:912037/1‑42 (MQ=255) tcGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGtgat > 1:1030171/1‑41 (MQ=255) tcGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGt < 1:4253430/38‑1 (MQ=255) tcGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGt < 1:2760253/38‑1 (MQ=255) tcGTTCGGTAAACACCCTGTTGGAATAAATAACGCGGtgatg > 1:1065264/1‑42 (MQ=255) cGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt > 1:4618268/1‑71 (MQ=255) cGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATAtt < 1:2015882/70‑1 (MQ=255) gTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt < 1:517938/70‑1 (MQ=255) ttCGGTAACCACCCTGTTGGAATAAATAACGCGGtgat < 1:1339191/38‑1 (MQ=255) ttCGGTAACCACCCTGTTGGAATAAATAACGCGGtgat < 1:4801529/38‑1 (MQ=255) ttCGGTAACCACCCTGTTGGAATAAATAACGCGGtgat < 1:821719/38‑1 (MQ=255) ttCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGatat < 1:4230686/67‑1 (MQ=255) cGGTAACCACCCTGTTGGAATAAATAACGCGGtga > 1:3114775/1‑35 (MQ=255) cGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGc < 1:4888212/70‑1 (MQ=255) gccaacCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGAtg < 1:2863261/67‑2 (MQ=255) ccaacCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCa > 1:4707540/1‑71 (MQ=255) caccCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGata > 1:255166/1‑57 (MQ=255) caccCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCAt > 1:2490011/1‑71 (MQ=255) caccCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCAt > 1:51359/1‑71 (MQ=255) caccCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCAt > 1:5082293/1‑71 (MQ=255) cTGTTGGAATAAATAACGCGGTGATGATACCCCAcgc < 1:2227238/37‑1 (MQ=255) cTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTg > 1:4581898/1‑71 (MQ=255) | CAGCAGCGGCAGGAGATAAGTGATCATCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTG > minE/2569006‑2569144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |