| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,101 | T→A | 7.8% | I32F (ATC→TTC) ‡ | mtlA ← | fused mannitol‑specific PTS enzyme IIABC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,101 | 0 | T | A | 7.8% | 171.3 / 6.2 | 64 | I32F (ATC→TTC) ‡ | mtlA | fused mannitol‑specific PTS enzyme IIABC components |
| Reads supporting (aligned to +/- strand): ref base T (30/29); new base A (3/2); total (33/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
TCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCACT > minE/2569032‑2569171 | tcGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATa < 1:2056632/71‑1 (MQ=255) aCCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCAcgc < 1:3845802/71‑1 (MQ=255) aCCAGCTTCCCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCAcgc < 1:1166793/71‑1 (MQ=255) ccAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCAcgc > 1:4679329/1‑70 (MQ=255) cAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGa > 1:67731/1‑71 (MQ=255) aGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGAt < 1:5698130/71‑1 (MQ=255) cTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATa > 1:2675980/1‑70 (MQ=255) ttCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATAccc > 1:4633505/1‑60 (MQ=255) ttCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATAccc > 1:648603/1‑60 (MQ=255) cGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAAcg > 1:2617703/1‑71 (MQ=255) cGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAAAAAATAACGCTGTGATGATACGCCACGCGATa < 1:29603/67‑1 (MQ=255) gCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAAcgc < 1:2425969/71‑1 (MQ=255) aGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGcc > 1:4570591/1‑71 (MQ=255) aGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGcc > 1:5613050/1‑71 (MQ=255) tctcGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGata < 1:5634626/71‑1 (MQ=255) ttcGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAata > 1:2891923/2‑70 (MQ=255) cGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt > 1:4618268/1‑71 (MQ=255) cGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATAtt < 1:2015882/70‑1 (MQ=255) gTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt < 1:517938/70‑1 (MQ=255) ttCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGatat < 1:4230686/67‑1 (MQ=255) cGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGc < 1:4888212/70‑1 (MQ=255) gccaacCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGAtg < 1:2863261/67‑2 (MQ=255) ccaacCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCa > 1:4707540/1‑71 (MQ=255) caccCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGata > 1:255166/1‑57 (MQ=255) caccCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCAt > 1:2490011/1‑71 (MQ=255) caccCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCAt > 1:5082293/1‑71 (MQ=255) caccCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCAt > 1:51359/1‑71 (MQ=255) cTGTTGGAATAAATAACGCGGTGATGATACCCCAcgc < 1:2227238/37‑1 (MQ=255) cTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTg > 1:4581898/1‑71 (MQ=255) ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGata < 1:4769913/50‑1 (MQ=255) ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt < 1:2644061/53‑1 (MQ=255) ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt < 1:292667/53‑1 (MQ=255) ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt < 1:491361/53‑1 (MQ=255) ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCt < 1:24541/70‑1 (MQ=255) ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCt < 1:1896580/70‑1 (MQ=255) ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCt < 1:2837901/70‑1 (MQ=255) ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTg < 1:5613654/71‑1 (MQ=255) ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTg < 1:618069/71‑1 (MQ=255) ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTg < 1:2391542/71‑1 (MQ=255) tgtaataaataACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCAc < 1:3122720/57‑1 (MQ=255) tGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCAc < 1:3154253/60‑1 (MQ=255) ggAATAAATAACGCGGTGATGATACCCCACGCGATa > 1:3203967/1‑36 (MQ=255) ggAATAAATAACGCGGTGATGATACCCCACGCGATa > 1:2979551/1‑36 (MQ=255) ggAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGata > 1:1594840/1‑48 (MQ=255) ggAATAAATAACGCAGTAATAAAACCCCAGGCAAAAAAAGCACCAata > 1:781574/1‑48 (MQ=255) gAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGata < 1:2249419/47‑1 (MQ=255) gAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGat > 1:372050/1‑46 (MQ=255) aataaataACGCGGTGATGATACCCCACGCGATAAACGCGACGATATTTGGCATCACCATGTTGCTGAgg > 1:22585/1‑70 (MQ=255) ataaataaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGa > 1:3994007/1‑70 (MQ=255) taaataaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt < 1:535203/47‑1 (MQ=255) aaataaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAAc > 1:4707280/1‑71 (MQ=255) aataaCGCGGTGATGATACCCCACGCGATAAACGCGCCGata > 1:1675760/1‑42 (MQ=255) aataaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTgg > 1:5385183/1‑47 (MQ=255) aataaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTg > 1:3337096/1‑46 (MQ=255) taaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCt < 1:3230517/60‑1 (MQ=255) taaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGAc > 1:502137/1‑71 (MQ=255) aaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATAtt > 1:4271999/1‑41 (MQ=255) aaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATAtt > 1:5348717/1‑41 (MQ=255) aCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGa > 1:2991964/1‑60 (MQ=255) cgcgGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCAt < 1:5310353/45‑1 (MQ=255) cgcgGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGAcc > 1:1017178/1‑69 (MQ=255) cgGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAg < 1:139966/71‑1 (MQ=255) tgatgaTACCCCACGCTATAAACGCGCCGATAttt < 1:3177720/35‑1 (MQ=255) tgatgaTACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCtt > 1:2149476/1‑71 (MQ=255) gatgatACCCCACGCGATAAACGAGCCGATATTTGGCATCACCATGTTGCTGAGGa < 1:2487797/56‑1 (MQ=255) atgatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTg < 1:231921/51‑1 (MQ=255) atgatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCttt > 1:834186/1‑70 (MQ=255) atgatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTg > 1:5212328/1‑71 (MQ=255) atgatACCACACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGaa < 1:5528332/56‑1 (MQ=255) gatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAAc > 1:3814279/1‑56 (MQ=255) gatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAAc > 1:2289835/1‑56 (MQ=255) gatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAAc > 1:2072208/1‑56 (MQ=255) gatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCa > 1:4830166/1‑71 (MQ=255) gatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCa > 1:3068868/1‑71 (MQ=255) gatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCa > 1:1368189/1‑71 (MQ=255) atACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCaaa > 1:2450131/1‑62 (MQ=255) atACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAg < 1:3611785/63‑1 (MQ=255) tACCCCACGCGATAAACGCGCCGATATTTGGCATc < 1:2147013/35‑1 (MQ=255) tACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCt < 1:2390696/64‑1 (MQ=255) tACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCACt < 1:821188/71‑1 (MQ=255) tACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCACt < 1:1097156/71‑1 (MQ=255) | TCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCACT > minE/2569032‑2569171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |