| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,781 | C→T | 10.9% | A364A (GCG→GCA) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,781 | 0 | C | T | 10.9% | 342.9 / 20.3 | 120 | A364A (GCG→GCA) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (42/62); new base T (7/6); total (52/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 122 total aligned reads displayed.
CAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTA > minE/311714‑311850 | aGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTcgcgcg > 1:4846186/1‑70 (MQ=255) aGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCgg < 1:3663923/71‑1 (MQ=255) ggcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGa < 1:2655864/71‑1 (MQ=255) gcggcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCACCGTCGCGCgg > 1:3497152/1‑69 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt < 1:1388741/71‑1 (MQ=255) aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg > 1:2082518/1‑71 (MQ=255) aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg < 1:1954891/71‑1 (MQ=255) aGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg > 1:2930044/1‑71 (MQ=255) ggCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGa < 1:3631523/71‑1 (MQ=255) aaGGTCCCCAGCAGCACCACCGGTACGGCGATGGTCGGGATCAGCGTGGCGCGGAAGTTCTGCAGGAACAg > 1:5215938/1‑71 (MQ=11) aaGGTCCCCAGCAGCACCACCGGTACGGCGATGGTCGGGATCAGCGTGGCGCGGAAGTTCTGCAGGAACAg > 1:5237249/1‑71 (MQ=11) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:2496179/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:13541/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:2566969/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:4773430/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:2961535/56‑1 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAg < 1:4473877/56‑1 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:4441074/1‑50 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa < 1:122985/64‑1 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACa < 1:3948426/70‑1 (MQ=255) cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAcatacata < 1:128063/71‑1 (MQ=255) cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa < 1:2566942/71‑1 (MQ=255) aacAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:4922309/3‑71 (MQ=2) aacAACACCACGGGTACCGCAATGGTGGGGATCAGAGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:5222363/3‑71 (MQ=2) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:2727410/1‑59 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:2609659/1‑59 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:2780819/1‑59 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc < 1:966593/71‑1 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc < 1:2970212/71‑1 (MQ=255) gCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAcc < 1:1008852/71‑1 (MQ=255) acAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:1975921/2‑70 (MQ=2) acAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:1661494/2‑70 (MQ=2) aacaccacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAg > 1:4596896/4‑71 (MQ=2) aaTACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:511256/65‑1 (MQ=255) accacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAgg < 1:1106224/69‑1 (MQ=9) accaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:822193/1‑41 (MQ=255) accaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:295208/1‑41 (MQ=255) accaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:2786853/1‑41 (MQ=255) ccaccGGTACGGCAATGGTCGGATTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:4568543/1‑70 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:1083101/1‑40 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:1165736/1‑40 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt > 1:4393492/1‑61 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:4377344/1‑70 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAac < 1:403968/71‑1 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGAAGGAACAg > 1:2907685/1‑55 (MQ=255) caccGGTACGGCAATGGTCGGAATCAACGTCGCGCgg > 1:4014668/1‑37 (MQ=255) caccGGTACGGCAATGGTCGGAATCAACGTCGCGCgg > 1:5433506/1‑37 (MQ=255) caccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc < 1:5398554/52‑1 (MQ=255) acgggTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAg > 1:3124164/4‑65 (MQ=2) acgggTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAacac < 1:5493422/68‑1 (MQ=9) accGGTACGGCAATGGTCGGAATCAACTTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac < 1:4566776/71‑1 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:4882378/66‑1 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac < 1:3584368/71‑1 (MQ=255) accGGCACCGCGATCGTCGGGATCAGCGTCGCCCGCCAATTCTGCAGGAACAGGAACATCACCAGGAagac < 1:4785712/71‑1 (MQ=2) ccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAg > 1:266931/1‑52 (MQ=255) ccGGTACGGCAATGGTAGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa > 1:4796944/1‑51 (MQ=255) ggTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg > 1:1448485/1‑71 (MQ=255) tACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGga > 1:2878751/1‑71 (MQ=255) aCGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgat > 1:4248622/1‑71 (MQ=255) ccgCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGcat < 1:786931/68‑1 (MQ=9) ccgCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGcat < 1:3775086/68‑1 (MQ=9) cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:109107/71‑1 (MQ=255) cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:4147432/71‑1 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc > 1:2497879/1‑42 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc > 1:1191143/1‑42 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatgat < 1:5089969/71‑1 (MQ=255) cAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAcc < 1:1768456/53‑1 (MQ=2) aaTGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGACCACCAGcataatg > 1:5560352/1‑69 (MQ=2) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGa > 1:1807280/1‑42 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:1387417/47‑1 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:1821335/47‑1 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa < 1:2646836/48‑1 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACg < 1:2180989/60‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:1423131/53‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:2015780/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:3730336/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:5267957/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:1524122/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:3154050/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:3283076/61‑1 (MQ=255) ggTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg > 1:4168476/1‑60 (MQ=255) ggTCGGAATCAACGTCGCGAGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCtt > 1:5645169/1‑71 (MQ=255) gTCGGATTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:4376172/59‑1 (MQ=255) cGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGa > 1:1014996/1‑71 (MQ=255) aaTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCa < 1:5031656/71‑1 (MQ=255) aTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAg > 1:1071591/1‑71 (MQ=255) tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACg < 1:1713518/50‑1 (MQ=255) tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:2094437/56‑1 (MQ=255) tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:1918509/56‑1 (MQ=255) aaCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:3908644/70‑1 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:4149973/40‑1 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt > 1:2754883/1‑71 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:3234591/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:3121986/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:1484380/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:950230/71‑1 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:1937636/66‑1 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:3918965/71‑1 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:1121907/71‑1 (MQ=255) tCGCGCGGAAGTTCTGAAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:2248828/66‑1 (MQ=255) | CAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTA > minE/311714‑311850 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |