| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,763 | G→T | 7.5% | L11L (CTG→CTT) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,763 | 0 | G | T | 7.5% | 509.9 / 15.2 | 158 | L11L (CTG→CTT) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (74/72); new base T (9/3); total (83/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.17e-01 | |||||||||||
Only 100 of 162 total aligned reads displayed.
CCAAAAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAA > minE/2569693‑2569831 | ccAAAAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTg < 1:4756782/71‑1 (MQ=255) cAAAAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGa > 1:683986/1‑71 (MQ=255) aaaaaCAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGAt > 1:3321649/1‑71 (MQ=255) aaaCAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCt > 1:4832575/1‑71 (MQ=255) aaaCAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCt > 1:5094323/1‑71 (MQ=255) aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCtt > 1:842408/1‑71 (MQ=255) aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCtt > 1:5352950/1‑71 (MQ=255) aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCtt > 1:3537512/1‑71 (MQ=255) aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCt > 1:4375772/1‑70 (MQ=255) caacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGAt > 1:3605055/1‑66 (MQ=255) caaaaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGa > 1:791402/1‑65 (MQ=255) aacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTGGCGTTGGGAGTGCTGATCTTCGt > 1:4646370/1‑71 (MQ=255) aacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCg > 1:3557853/1‑70 (MQ=255) cacTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTg < 1:2813762/61‑1 (MQ=255) gATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt > 1:1311394/1‑71 (MQ=255) aTAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt < 1:3256525/70‑1 (MQ=255) tAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg > 1:2546584/1‑70 (MQ=255) aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTgg < 1:4648967/70‑1 (MQ=255) aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:5505269/1‑71 (MQ=255) aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:106797/1‑71 (MQ=255) aaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:5635951/1‑70 (MQ=255) aCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGa < 1:4766492/71‑1 (MQ=255) tGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCtt < 1:2832469/60‑1 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCttt > 1:1265371/1‑59 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCttt > 1:3039435/1‑59 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc > 1:1949962/1‑60 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:945975/71‑1 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATATCGCGTTGGGAGTGCTGATCTTCGt > 1:1106759/1‑55 (MQ=255) aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt < 1:5032271/71‑1 (MQ=255) tttgAGGGAAATATGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTgg > 1:3678299/4‑61 (MQ=255) tCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc < 1:4213653/58‑1 (MQ=255) cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg > 1:2014749/1‑71 (MQ=255) cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg > 1:313801/1‑71 (MQ=255) aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg > 1:3293467/1‑70 (MQ=255) aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa < 1:1007464/71‑1 (MQ=255) ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa < 1:5426406/71‑1 (MQ=255) ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa < 1:4966574/71‑1 (MQ=255) cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:2975868/67‑1 (MQ=255) gATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcat > 1:3468664/1‑71 (MQ=255) atatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc < 1:4388811/71‑1 (MQ=255) atatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGt > 1:5566425/1‑52 (MQ=255) tatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:5279947/58‑1 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:162784/1‑50 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:334821/1‑50 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:4197234/1‑50 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc > 1:2824968/1‑71 (MQ=255) tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCt < 1:820924/36‑1 (MQ=255) tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt < 1:260697/55‑1 (MQ=255) ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:1112452/1‑69 (MQ=255) ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:724577/1‑69 (MQ=255) tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc > 1:1649948/1‑41 (MQ=255) tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa < 1:5518815/70‑1 (MQ=255) cTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa > 1:5613240/1‑71 (MQ=255) tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt < 1:3014191/53‑1 (MQ=255) tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa > 1:1577962/1‑70 (MQ=255) tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt > 1:1824320/1‑71 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGTTGATTTTTTATGGAATCATCGCGATACATGa < 1:4099609/71‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt < 1:5147232/51‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt < 1:3771123/69‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt < 1:1428015/69‑1 (MQ=255) tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttt < 1:4214106/44‑1 (MQ=255) tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttt < 1:5078520/44‑1 (MQ=255) tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:1665762/50‑1 (MQ=255) tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:4518434/50‑1 (MQ=255) tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:4727819/50‑1 (MQ=255) tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:525027/1‑45 (MQ=255) tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:4201287/1‑45 (MQ=255) tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg < 1:2016093/47‑1 (MQ=255) tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc > 1:5028595/1‑55 (MQ=255) tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt > 1:320574/1‑71 (MQ=255) cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:2830359/45‑1 (MQ=255) cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt > 1:2503064/1‑70 (MQ=255) gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt < 1:1162472/70‑1 (MQ=255) gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATc > 1:1804155/1‑71 (MQ=255) ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt < 1:2308373/39‑1 (MQ=255) ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:2252363/71‑1 (MQ=255) ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat < 1:2136477/46‑1 (MQ=255) ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat < 1:3523727/46‑1 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:3678832/35‑1 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATAc > 1:4174044/1‑54 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa > 1:58755/1‑58 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:644274/1‑70 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:259420/1‑36 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:1899228/1‑36 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTg < 1:552288/70‑1 (MQ=255) ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa < 1:141501/70‑1 (MQ=255) ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa < 1:222583/70‑1 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:726336/44‑1 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:696116/1‑46 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc < 1:812098/71‑1 (MQ=255) tGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGc > 1:4424675/1‑70 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:3392378/46‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:2991637/46‑1 (MQ=255) gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:1895950/63‑1 (MQ=255) gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:2916058/63‑1 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt < 1:4868967/58‑1 (MQ=255) cTGATCTTCGTCTCTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa > 1:5255712/1‑70 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca > 1:5554549/1‑36 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca > 1:2386579/1‑36 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc < 1:777398/67‑1 (MQ=255) | CCAAAAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAA > minE/2569693‑2569831 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |