| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,766 | C→T | 7.5% | I12I (ATC→ATT) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,766 | 0 | C | T | 7.5% | 471.1 / 17.9 | 160 | I12I (ATC→ATT) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (73/75); new base T (9/3); total (82/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
Only 100 of 163 total aligned reads displayed.
AAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAG > minE/2569697‑2569835 | aaaCAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCt < 1:59929/71‑1 (MQ=255)aaaCAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCt > 1:5094323/1‑71 (MQ=255) aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCtt > 1:842408/1‑71 (MQ=255) aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCtt > 1:3537512/1‑71 (MQ=255) aacaacACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCtt > 1:5352950/1‑71 (MQ=255) aacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTGGCGTTGGGAGTGCTGATCTTCGt > 1:4646370/1‑71 (MQ=255) aacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCg > 1:3557853/1‑70 (MQ=255) aacaCTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGt > 1:1656650/1‑71 (MQ=255) cacTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCt < 1:2042810/71‑1 (MQ=255) gATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt > 1:1311394/1‑71 (MQ=255) aTAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt < 1:3256525/70‑1 (MQ=255) aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTgg < 1:4648967/70‑1 (MQ=255) aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:2818754/1‑71 (MQ=255) aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:106797/1‑71 (MQ=255) aaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:5635951/1‑70 (MQ=255) aCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGa < 1:4209238/71‑1 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCttt > 1:1265371/1‑59 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:945975/71‑1 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:5611426/71‑1 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt > 1:3293845/1‑71 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATATCGCGTTGGGAGTGCTGATCTTCGt > 1:1106759/1‑55 (MQ=255) aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt < 1:3006193/71‑1 (MQ=255) aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt < 1:5032271/71‑1 (MQ=255) aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt > 1:687717/1‑70 (MQ=255) tttgAGGGAAATATGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTgg > 1:3678299/4‑61 (MQ=255) tCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc < 1:4213653/58‑1 (MQ=255) tCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:3368057/1‑71 (MQ=255) cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg > 1:3068534/1‑58 (MQ=255) cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg > 1:2014749/1‑71 (MQ=255) cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg > 1:313801/1‑71 (MQ=255) aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa < 1:1007464/71‑1 (MQ=255) ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa < 1:4966574/71‑1 (MQ=255) ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa < 1:5426406/71‑1 (MQ=255) cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:2975868/67‑1 (MQ=255) cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc < 1:4377304/71‑1 (MQ=255) cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc < 1:2532546/71‑1 (MQ=255) aatatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat > 1:70260/2‑71 (MQ=255) atatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc < 1:4388811/71‑1 (MQ=255) atatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGt > 1:19087/1‑52 (MQ=255) atatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGt > 1:5566425/1‑52 (MQ=255) tatGTTCCTGGACTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGTGAt > 1:886156/1‑54 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg > 1:26027/1‑48 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:4197234/1‑50 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:162784/1‑50 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:5109092/1‑50 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:3513330/1‑50 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:3283458/1‑59 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc > 1:2824968/1‑71 (MQ=255) tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:417653/1‑58 (MQ=255) tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:4112691/70‑1 (MQ=255) gTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:1796788/1‑71 (MQ=255) tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt < 1:4360511/55‑1 (MQ=255) ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:1518927/1‑55 (MQ=255) ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:1112452/1‑69 (MQ=255) ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:724577/1‑69 (MQ=255) tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc > 1:3589946/1‑41 (MQ=255) tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg < 1:5540174/56‑1 (MQ=255) tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa < 1:5518815/70‑1 (MQ=255) tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt < 1:3014191/53‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt < 1:1428015/69‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt < 1:3771123/69‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa < 1:5066146/71‑1 (MQ=255) tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:4727819/50‑1 (MQ=255) ttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc > 1:217316/1‑51 (MQ=255) tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt > 1:320574/1‑71 (MQ=255) cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:4756952/45‑1 (MQ=255) cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:1957759/45‑1 (MQ=255) cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:2830359/45‑1 (MQ=255) gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt < 1:1162472/70‑1 (MQ=255) gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATc > 1:1804155/1‑71 (MQ=255) ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt < 1:5277875/39‑1 (MQ=255) ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat < 1:3523727/46‑1 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:2437320/35‑1 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa > 1:58755/1‑58 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:1899228/1‑36 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:259420/1‑36 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTg < 1:552288/70‑1 (MQ=255) ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGAt > 1:4515618/1‑71 (MQ=255) ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa < 1:5462382/70‑1 (MQ=255) ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa < 1:222583/70‑1 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:2265264/1‑46 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc < 1:812098/71‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:3711333/46‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:1850571/46‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:3392378/46‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:4820180/64‑1 (MQ=255) gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa < 1:2854505/51‑1 (MQ=255) gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:2916058/63‑1 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt < 1:4868967/58‑1 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaa < 1:502164/71‑1 (MQ=255) cTGATCTTCGTCTCTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa > 1:5255712/1‑70 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca > 1:2386579/1‑36 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa > 1:743848/1‑51 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa > 1:815750/1‑51 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc < 1:777398/67‑1 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc < 1:1624129/67‑1 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcat > 1:892416/1‑35 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa < 1:806317/69‑1 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTAGCTGATTGCCaaa > 1:5178328/1‑69 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:1338825/1‑60 (MQ=255) | AAACAACACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAG > minE/2569697‑2569835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |