| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,772 | C→A | 6.8% | V14V (GTC→GTA) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,772 | 0 | C | A | 6.8% | 556.1 / 12.2 | 171 | V14V (GTC→GTA) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (71/88); new base A (9/3); total (80/91) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.86e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 | |||||||||||
Only 100 of 172 total aligned reads displayed.
CACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAC > minE/2569703‑2569841 | cacTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCt < 1:2042810/71‑1 (MQ=255) gATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt > 1:1311394/1‑71 (MQ=255) aTAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCt < 1:3256525/70‑1 (MQ=255) tAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTgg > 1:3672462/1‑71 (MQ=255) aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTgg < 1:4648967/70‑1 (MQ=255) aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:106797/1‑71 (MQ=255) aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:5505269/1‑71 (MQ=255) aaaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:2818754/1‑71 (MQ=255) aaCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:5635951/1‑70 (MQ=255) aCGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGa < 1:4209238/71‑1 (MQ=255) tGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCtt < 1:2832469/60‑1 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc > 1:1949962/1‑60 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:945975/71‑1 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:5611426/71‑1 (MQ=255) aaTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt > 1:3293845/1‑71 (MQ=255) aTCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt < 1:5032271/71‑1 (MQ=255) tCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc < 1:4213653/58‑1 (MQ=255) ccGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt > 1:2937627/1‑71 (MQ=255) cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg > 1:3068534/1‑58 (MQ=255) cGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg > 1:2014749/1‑71 (MQ=255) aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg > 1:3293467/1‑70 (MQ=255) aGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGa < 1:1007464/71‑1 (MQ=255) ggCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGaa < 1:5426406/71‑1 (MQ=255) cAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc < 1:2532546/71‑1 (MQ=255) gATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTc < 1:2825236/49‑1 (MQ=255) atatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc < 1:4388811/71‑1 (MQ=255) tatGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:5279947/58‑1 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTg > 1:26027/1‑48 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:162784/1‑50 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:4197234/1‑50 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:334821/1‑50 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:5109092/1‑50 (MQ=255) atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGt > 1:3513330/1‑50 (MQ=255) tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:571171/70‑1 (MQ=255) gTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:1796788/1‑71 (MQ=255) tttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGAtttttt < 1:4360511/55‑1 (MQ=255) ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:1518927/1‑55 (MQ=255) ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:724577/1‑69 (MQ=255) ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:1112452/1‑69 (MQ=255) tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg < 1:5540174/56‑1 (MQ=255) tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt < 1:3014191/53‑1 (MQ=255) tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt > 1:1824320/1‑71 (MQ=255) gaCTATTTTGCACTGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGat > 1:5522801/2‑56 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGTTGATTTTTTATGGAATCATCGCGATACATGa < 1:4099609/71‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATg > 1:1265138/1‑52 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt < 1:1428015/69‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa < 1:5066146/71‑1 (MQ=255) tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:4518434/50‑1 (MQ=255) tATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:1665762/50‑1 (MQ=255) tttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:525027/1‑45 (MQ=255) ttCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatc > 1:217316/1‑51 (MQ=255) tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt > 1:320574/1‑71 (MQ=255) cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:1957759/45‑1 (MQ=255) cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATgg < 1:4756952/45‑1 (MQ=255) cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt > 1:2503064/1‑70 (MQ=255) gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt < 1:1162472/70‑1 (MQ=255) ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTAt < 1:2308373/39‑1 (MQ=255) ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:2252363/71‑1 (MQ=255) ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat < 1:3523727/46‑1 (MQ=255) ctGGGAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGatcat < 1:2136477/46‑1 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:3678832/35‑1 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGAttttt < 1:2437320/35‑1 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATAc > 1:4174044/1‑54 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:644274/1‑70 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:1899228/1‑36 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTa > 1:259420/1‑36 (MQ=255) ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa < 1:141501/70‑1 (MQ=255) gAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca > 1:3162746/1‑42 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:726336/44‑1 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:696116/1‑46 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc < 1:812098/71‑1 (MQ=255) tGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGc > 1:4424675/1‑70 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:1850571/46‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:3392378/46‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:2991637/46‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:3711333/46‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:4820180/64‑1 (MQ=255) gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa < 1:2854505/51‑1 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt < 1:4868967/58‑1 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaa < 1:502164/71‑1 (MQ=255) cTGATCTTCGTCTCTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa > 1:5255712/1‑70 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAatca > 1:5554549/1‑36 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa > 1:743848/1‑51 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc < 1:1624129/67‑1 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcatc < 1:3542428/36‑1 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAatcat > 1:892416/1‑35 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa < 1:806317/69‑1 (MQ=255) tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:4984488/1‑71 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:1338825/1‑60 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:865341/1‑60 (MQ=255) tCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:3704124/1‑36 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:2987201/1‑35 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:5167353/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:467712/70‑1 (MQ=255) gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa < 1:5359199/70‑1 (MQ=255) gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa < 1:789457/70‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATccc < 1:4018103/46‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:4690396/68‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc > 1:782962/1‑71 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc < 1:851204/71‑1 (MQ=255) | CACTGATAAACGTGAATCCGAGGCAGATATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAC > minE/2569703‑2569841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |