| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,800 | G→A | 8.5% | A24T (GCG→ACG) ‡ | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,800 | 0 | G | A | 8.5% | 401.0 / 17.2 | 140 | A24T (GCG→ACG) ‡ | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (65/63); new base A (9/3); total (74/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 154 total aligned reads displayed.
ATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGC > minE/2569731‑2569870 | atGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc > 1:2824968/1‑71 (MQ=255) tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:571171/70‑1 (MQ=255) tGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATcgc < 1:4112691/70‑1 (MQ=255) ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:1112452/1‑69 (MQ=255) ttCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:724577/1‑69 (MQ=255) tCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATa < 1:5518815/70‑1 (MQ=255) cTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa > 1:5613240/1‑71 (MQ=255) tAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACa > 1:1577962/1‑70 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGTTGATTTTTTATGGAATCATCGCGATACATGa < 1:4099609/71‑1 (MQ=255) aaCTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACAt < 1:1428015/69‑1 (MQ=255) tCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt > 1:320574/1‑71 (MQ=255) cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt > 1:2503064/1‑70 (MQ=255) cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt > 1:1312826/1‑70 (MQ=255) gcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt < 1:1162472/70‑1 (MQ=255) gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATc > 1:1804155/1‑71 (MQ=255) ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:2252363/71‑1 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATAc > 1:4174044/1‑54 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGa > 1:58755/1‑58 (MQ=255) gggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTg < 1:552288/70‑1 (MQ=255) ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGAt > 1:4515618/1‑71 (MQ=255) ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa < 1:141501/70‑1 (MQ=255) ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa < 1:222583/70‑1 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:696116/1‑46 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:2265264/1‑46 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc < 1:812098/71‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:3711333/46‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGAt < 1:1850571/46‑1 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:4820180/64‑1 (MQ=255) gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:1895950/63‑1 (MQ=255) gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:2916058/63‑1 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaa < 1:502164/71‑1 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACa > 1:743848/1‑51 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa < 1:806317/69‑1 (MQ=255) tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:4984488/1‑71 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:1338825/1‑60 (MQ=255) tCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:3704124/1‑36 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGa > 1:2987201/1‑35 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:5167353/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:467712/70‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc > 1:3327599/1‑70 (MQ=255) gtatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa > 1:1521763/4‑71 (MQ=255) gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa < 1:789457/70‑1 (MQ=255) gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa < 1:5359199/70‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATccc < 1:4018103/46‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:1982204/52‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:4594536/52‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:735993/68‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc < 1:851204/71‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc > 1:2122634/1‑71 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc > 1:782962/1‑71 (MQ=255) cTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc > 1:1727243/1‑70 (MQ=255) atttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat > 1:253195/2‑69 (MQ=255) tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa < 1:4724949/67‑1 (MQ=255) tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa > 1:2794752/1‑71 (MQ=255) tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa > 1:2630246/1‑71 (MQ=255) tttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCa > 1:4881032/1‑71 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc < 1:3938435/68‑1 (MQ=255) tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCaaaaaa > 1:1709133/1‑60 (MQ=255) tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa > 1:1493973/1‑66 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc < 1:4729617/70‑1 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:2765947/69‑1 (MQ=255) gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa < 1:3876165/71‑1 (MQ=255) gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa < 1:1255475/71‑1 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt > 1:2705846/1‑71 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt > 1:4860180/1‑71 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt > 1:5042565/1‑71 (MQ=255) tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa < 1:2109396/50‑1 (MQ=255) ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa > 1:2328510/1‑40 (MQ=255) ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa > 1:1020948/1‑40 (MQ=255) ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa > 1:1130441/1‑40 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:3092708/1‑61 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:5449866/1‑61 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:3497020/1‑61 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:2437681/1‑49 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:3549900/1‑49 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc > 1:5043405/1‑70 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg > 1:1469594/1‑71 (MQ=255) aTGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc > 1:3002362/1‑70 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:4703683/1‑44 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:2644590/1‑44 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt > 1:4691944/1‑71 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt > 1:2599164/1‑71 (MQ=255) aaTCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa > 1:4549641/1‑42 (MQ=255) aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:1603628/61‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:906763/67‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:3408617/67‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:3992792/1‑70 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt < 1:1797360/71‑1 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:5237444/1‑39 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:852705/1‑70 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCaa < 1:5087400/43‑1 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg > 1:1004806/1‑60 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg > 1:3178585/1‑71 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCGACCATCCCCATGCCGACGCTATTc > 1:5203480/1‑65 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:3722708/1‑57 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:2711969/1‑57 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:2050557/1‑57 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt > 1:1287173/1‑64 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCAAAAAAGCGCAACCATCCCCATGCCGa > 1:1172349/1‑57 (MQ=255) gcgATACATGACATCCCTTATCTGATTGCCAAAAAGAGCAACTATCCCCATGCCGACGCTATTCATAcggc > 1:2421094/1‑71 (MQ=255) | ATGTTTCTAAACTATTTCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGC > minE/2569731‑2569870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |