| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,814 | C→T | 10.9% | I28I (ATC→ATT) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,814 | 0 | C | T | 10.9% | 397.8 / 28.0 | 147 | I28I (ATC→ATT) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (63/68); new base T (9/7); total (72/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
Only 100 of 148 total aligned reads displayed.
TCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGC > minE/2569747‑2569883 | cgcgTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCt > 1:2503064/1‑70 (MQ=255) ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:2252363/71‑1 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:644274/1‑70 (MQ=255) ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa < 1:5462382/70‑1 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc < 1:812098/71‑1 (MQ=255) tGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGc > 1:4424675/1‑70 (MQ=255) tGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:4820180/64‑1 (MQ=255) gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:1895950/63‑1 (MQ=255) cTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCtt < 1:4868967/58‑1 (MQ=255) cTGATCTTCGTCTCTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCa > 1:5255712/1‑70 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc < 1:777398/67‑1 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaa < 1:806317/69‑1 (MQ=255) aTCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTAGCTGATTGCCaaa > 1:5178328/1‑69 (MQ=255) tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:3244098/1‑71 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:865341/1‑60 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:1338825/1‑60 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:1358557/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:5167353/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:467712/70‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc > 1:3327599/1‑70 (MQ=255) gtatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa > 1:1521763/4‑71 (MQ=255) tatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa < 1:27112/68‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:1449400/52‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:4690396/68‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:4991647/68‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc > 1:782962/1‑71 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc > 1:2122634/1‑71 (MQ=255) atttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat > 1:253195/2‑69 (MQ=255) tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa < 1:4724949/67‑1 (MQ=255) tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa > 1:2630246/1‑71 (MQ=255) tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa > 1:2794752/1‑71 (MQ=255) tttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCa > 1:4881032/1‑71 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa < 1:2648889/69‑1 (MQ=255) tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCaaaaaa > 1:1709133/1‑60 (MQ=255) tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa > 1:1493973/1‑66 (MQ=255) tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc > 1:4578061/1‑71 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc < 1:4729617/70‑1 (MQ=255) gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa < 1:3876165/71‑1 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt > 1:5042565/1‑71 (MQ=255) aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa > 1:4173963/1‑52 (MQ=255) aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg < 1:4031945/70‑1 (MQ=255) aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg < 1:5529270/70‑1 (MQ=255) tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa < 1:2319478/50‑1 (MQ=255) tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa < 1:2109396/50‑1 (MQ=255) ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa > 1:1020948/1‑40 (MQ=255) ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa > 1:2328510/1‑40 (MQ=255) ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa > 1:1130441/1‑40 (MQ=255) ttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:190146/1‑51 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:224250/1‑50 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:3969232/1‑50 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:3092708/1‑61 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:5449866/1‑61 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:3497020/1‑61 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:2437681/1‑49 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:3549900/1‑49 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCg > 1:114063/1‑68 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc > 1:5043405/1‑70 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg > 1:797799/1‑71 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg > 1:1469594/1‑71 (MQ=255) aTGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc > 1:3002362/1‑70 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:4703683/1‑44 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt > 1:2599164/1‑71 (MQ=255) aaTCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa > 1:4549641/1‑42 (MQ=255) aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:1603628/61‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:2111433/67‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:3408617/67‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:3992792/1‑70 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt < 1:1797360/71‑1 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:5237444/1‑39 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:2776419/65‑1 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa < 1:3140025/68‑1 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:852705/1‑70 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:1243472/1‑70 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg > 1:1004806/1‑60 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg > 1:3178585/1‑71 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:2711969/1‑57 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:2050557/1‑57 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt > 1:1287173/1‑64 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc > 1:1299216/1‑65 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCAAAAAAGCGCAACCATCCCCATGCCGa > 1:1172349/1‑57 (MQ=255) gcgATACATGACATCCCTTATCTGATTGCCAAAAAGAGCAACTATCCCCATGCCGACGCTATTCATAcggc > 1:2421094/1‑71 (MQ=255) gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:576693/46‑1 (MQ=255) gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcgg < 1:4708106/71‑1 (MQ=255) tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc < 1:2005412/67‑1 (MQ=255) tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc < 1:1745259/67‑1 (MQ=255) cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg < 1:1092572/52‑1 (MQ=255) cATGACGTCCCTTATCTGATTGCCAAAAAGCGCAACCATcc < 1:3461658/41‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:3715975/42‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:1594965/42‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:477828/1‑55 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:1651559/1‑55 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCt < 1:2381209/70‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg > 1:4990284/1‑71 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg > 1:2695250/1‑71 (MQ=255) aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:2625883/47‑1 (MQ=255) aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:4485198/47‑1 (MQ=255) aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg < 1:812775/71‑1 (MQ=255) tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTggg < 1:3918818/71‑1 (MQ=255) aTCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa < 1:4898262/49‑1 (MQ=255) tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa < 1:700276/48‑1 (MQ=255) | TCGCGTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGC > minE/2569747‑2569883 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |