| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,820 | T→C | 9.7% | Y30Y (TAT→TAC) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,820 | 0 | T | C | 9.7% | 456.6 / 28.2 | 168 | Y30Y (TAT→TAC) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (79/72); new base C (9/7); total (89/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
Only 100 of 170 total aligned reads displayed.
GTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTT > minE/2569751‑2569888 | gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATc > 1:1804155/1‑71 (MQ=255) ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:2252363/71‑1 (MQ=255) tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt > 1:644274/1‑70 (MQ=255) ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGAt > 1:4515618/1‑71 (MQ=255) ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa < 1:5462382/70‑1 (MQ=255) ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa < 1:222583/70‑1 (MQ=255) ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa < 1:141501/70‑1 (MQ=255) gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc < 1:812098/71‑1 (MQ=255) gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:1895950/63‑1 (MQ=255) tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc < 1:1624129/67‑1 (MQ=255) tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:4984488/1‑71 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:1338825/1‑60 (MQ=255) cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt > 1:865341/1‑60 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:2519598/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa < 1:5167353/66‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:467712/70‑1 (MQ=255) cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc > 1:3327599/1‑70 (MQ=255) gtatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa > 1:1521763/4‑71 (MQ=255) gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa < 1:789457/70‑1 (MQ=255) gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa < 1:5359199/70‑1 (MQ=255) tatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa < 1:27112/68‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt < 1:4594536/52‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:735993/68‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:4991647/68‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:4690396/68‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc < 1:851204/71‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc > 1:782962/1‑71 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc > 1:2122634/1‑71 (MQ=255) tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa < 1:4724949/67‑1 (MQ=255) tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa > 1:2630246/1‑71 (MQ=255) tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa > 1:2794752/1‑71 (MQ=255) tttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCa > 1:4881032/1‑71 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc < 1:3938435/68‑1 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa < 1:2648889/69‑1 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc > 1:585691/1‑71 (MQ=255) tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCaaaaaa > 1:1709133/1‑60 (MQ=255) tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa > 1:1493973/1‑66 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc < 1:4729617/70‑1 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc < 1:2434928/70‑1 (MQ=255) gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa < 1:1255475/71‑1 (MQ=255) gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa < 1:3876165/71‑1 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt > 1:4860180/1‑71 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt > 1:5042565/1‑71 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt > 1:2705846/1‑71 (MQ=255) aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa > 1:4173963/1‑52 (MQ=255) aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg < 1:5529270/70‑1 (MQ=255) tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa < 1:2319478/50‑1 (MQ=255) tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa < 1:2109396/50‑1 (MQ=255) ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa > 1:1130441/1‑40 (MQ=255) ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa > 1:1020948/1‑40 (MQ=255) ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa > 1:2328510/1‑40 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:3092708/1‑61 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:3497020/1‑61 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCg > 1:114063/1‑68 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc > 1:5043405/1‑70 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg > 1:1469594/1‑71 (MQ=255) tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa < 1:2605352/71‑1 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt > 1:2599164/1‑71 (MQ=255) aaTCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa > 1:4549641/1‑42 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:3992792/1‑70 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:5237444/1‑39 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAg > 1:3527576/1‑39 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:2776419/65‑1 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:1243472/1‑70 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg > 1:1004806/1‑60 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg > 1:3178585/1‑71 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCGACCATCCCCATGCCGACGCTATTc > 1:5203480/1‑65 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:3722708/1‑57 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:2050557/1‑57 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt > 1:1287173/1‑64 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCAAAAAAGCGCAACCATCCCCATGCCGa > 1:1172349/1‑57 (MQ=255) gcgATACATGACATCCCTTATCTGATTGCCAAAAAGAGCAACTATCCCCATGCCGACGCTATTCATAcggc > 1:2421094/1‑71 (MQ=255) gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcgg < 1:4708106/71‑1 (MQ=255) tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc < 1:2350146/55‑1 (MQ=255) tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc < 1:2005412/67‑1 (MQ=255) tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc < 1:1745259/67‑1 (MQ=255) cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg < 1:811494/52‑1 (MQ=255) cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg < 1:1092572/52‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:1594965/42‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:3715975/42‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:477828/1‑55 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:1651559/1‑55 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg > 1:3457701/1‑66 (MQ=255) tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc < 1:4346774/71‑1 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:2946378/1‑41 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:3127835/1‑41 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt > 1:3402966/1‑51 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt > 1:4697492/1‑51 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc < 1:3576495/69‑1 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt > 1:2166381/1‑71 (MQ=255) cTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg > 1:4444284/1‑71 (MQ=255) ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:649719/1‑44 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:1926275/1‑38 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:3921082/1‑38 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg < 1:2734561/40‑1 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt > 1:3100864/1‑71 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:3741527/1‑42 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:1366089/1‑46 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt > 1:5463544/1‑70 (MQ=255) tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcgg > 1:802442/1‑50 (MQ=255) | GTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTT > minE/2569751‑2569888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |