| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,838 | C→T | 18.6% | R36R (CGC→CGT) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,838 | 0 | C | T | 18.6% | 365.5 / 66.7 | 166 | R36R (CGC→CGT) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (79/56); new base T (20/11); total (99/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
Only 100 of 174 total aligned reads displayed.
CGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTG > minE/2569769‑2569906 | cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:467712/70‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc < 1:4991647/68‑1 (MQ=255) tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc > 1:782962/1‑71 (MQ=255) atttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat > 1:253195/2‑69 (MQ=255) tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa < 1:4724949/67‑1 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa < 1:2648889/69‑1 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc > 1:585691/1‑71 (MQ=255) tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa > 1:1493973/1‑66 (MQ=255) tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc > 1:4578061/1‑71 (MQ=255) tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc > 1:2103782/1‑71 (MQ=255) gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa < 1:3876165/71‑1 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt > 1:2705846/1‑71 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt > 1:4860180/1‑71 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:3092708/1‑61 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:5449866/1‑61 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc > 1:5043405/1‑70 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg > 1:797799/1‑71 (MQ=255) aTGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc > 1:3002362/1‑70 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt > 1:2599164/1‑71 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt > 1:4691944/1‑71 (MQ=255) aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:4775917/61‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:2111433/67‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:3992792/1‑70 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:2776419/65‑1 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa < 1:3140025/68‑1 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:1243472/1‑70 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:852705/1‑70 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa < 1:1477907/70‑1 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCaa < 1:5087400/43‑1 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg > 1:1004806/1‑60 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg > 1:3178585/1‑71 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:2711969/1‑57 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc > 1:1299216/1‑65 (MQ=255) gcgATACATGACATCCCTTATCTGATTGCCAAAAAGAGCAACTATCCCCATGCCGACGCTATTCATAcggc > 1:2421094/1‑71 (MQ=255) gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcgg < 1:4708106/71‑1 (MQ=255) tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc < 1:2350146/55‑1 (MQ=255) tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc < 1:1745259/67‑1 (MQ=255) tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc < 1:3118730/67‑1 (MQ=255) tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc < 1:2005412/67‑1 (MQ=255) cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg < 1:1127274/52‑1 (MQ=255) cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg < 1:2316378/52‑1 (MQ=255) cATGACGTCCCTTATCTGATTGCCAAAAAGCGCAACCATcc < 1:3461658/41‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:1594965/42‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:3715975/42‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:1651559/1‑55 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg > 1:3457701/1‑66 (MQ=255) aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:2625883/47‑1 (MQ=255) aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg < 1:812775/71‑1 (MQ=255) tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc < 1:4346774/71‑1 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg < 1:1170258/37‑1 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:2946378/1‑41 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt > 1:3402966/1‑51 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt > 1:2166381/1‑71 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt < 1:2332947/71‑1 (MQ=255) cTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg > 1:4444284/1‑71 (MQ=255) ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:1282397/1‑44 (MQ=255) ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGt > 1:2197050/1‑71 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:3921082/1‑38 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:608827/1‑38 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:1926275/1‑38 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg < 1:2734561/40‑1 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc > 1:4624941/1‑46 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg < 1:3690337/60‑1 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt > 1:3801171/1‑71 (MQ=255) accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt > 1:4720228/3‑70 (MQ=255) accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt > 1:1425786/3‑70 (MQ=255) accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt > 1:5464106/3‑70 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt < 1:1084463/70‑1 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCAATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt > 1:1190558/1‑70 (MQ=255) tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcgg > 1:802442/1‑50 (MQ=255) tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt < 1:3732014/69‑1 (MQ=255) ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGa > 1:831240/2‑36 (MQ=16) ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt < 1:3537745/68‑1 (MQ=255) cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTtttt < 1:4888679/69‑1 (MQ=255) tGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt < 1:2790608/41‑1 (MQ=255) tGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt < 1:4778394/41‑1 (MQ=255) gATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt > 1:172019/1‑69 (MQ=255) aTTGCCAAAAAGCGCAACCATCCTCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTAc < 1:5367204/68‑1 (MQ=255) aTTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCa > 1:5217061/1‑41 (MQ=19) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct > 1:3853554/1‑70 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg > 1:1663345/1‑71 (MQ=255) gCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTa > 1:3065113/1‑64 (MQ=255) cAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATg > 1:4926543/1‑71 (MQ=255) aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt > 1:3214145/1‑71 (MQ=255) aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt > 1:1269569/1‑71 (MQ=255) aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt > 1:660056/1‑71 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCAt < 1:2710445/35‑1 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTa > 1:933933/1‑60 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTa > 1:5133009/1‑60 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt > 1:722329/1‑70 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt > 1:435764/1‑70 (MQ=255) aaaaGCGCAACCATCCCCAAGCCGACGCTATTCATAcggcgg > 1:2823040/1‑42 (MQ=255) aaaaACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt > 1:4942626/1‑70 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg > 1:2292439/1‑45 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg > 1:780185/1‑45 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt > 1:1535867/1‑71 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt > 1:1164799/1‑71 (MQ=255) aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:5631368/71‑1 (MQ=255) aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:5297502/71‑1 (MQ=255) cgcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTg > 1:4147305/1‑71 (MQ=255) | CGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTG > minE/2569769‑2569906 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |