| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,841 | C→T | 16.2% | N37N (AAC→AAT) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,841 | 0 | C | T | 16.2% | 366.4 / 50.8 | 154 | N37N (AAC→AAT) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (77/52); new base T (15/10); total (92/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
Only 100 of 168 total aligned reads displayed.
TCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGT > minE/2569771‑2569911 | tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc < 1:851204/71‑1 (MQ=255) cTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc > 1:1727243/1‑70 (MQ=255) tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa > 1:2794752/1‑71 (MQ=255) tttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCa > 1:4881032/1‑71 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc < 1:3938435/68‑1 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa < 1:2648889/69‑1 (MQ=255) tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc > 1:585691/1‑71 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc < 1:4729617/70‑1 (MQ=255) gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:2765947/69‑1 (MQ=255) gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa < 1:3876165/71‑1 (MQ=255) gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa < 1:1255475/71‑1 (MQ=255) tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt > 1:2705846/1‑71 (MQ=255) aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg < 1:4031945/70‑1 (MQ=255) aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg < 1:5529270/70‑1 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:5449866/1‑61 (MQ=255) tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc > 1:3092708/1‑61 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc > 1:5043405/1‑70 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg > 1:797799/1‑71 (MQ=255) ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg > 1:1469594/1‑71 (MQ=255) aTGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc > 1:3002362/1‑70 (MQ=255) tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa < 1:2605352/71‑1 (MQ=255) gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt > 1:4691944/1‑71 (MQ=255) aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:4775917/61‑1 (MQ=255) aaTCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:1603628/61‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:2111433/67‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:3408617/67‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:906763/67‑1 (MQ=255) tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt < 1:1797360/71‑1 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt < 1:2776419/65‑1 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa < 1:3140025/68‑1 (MQ=255) atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa > 1:1243472/1‑70 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg > 1:1004806/1‑60 (MQ=255) tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg > 1:3178585/1‑71 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:2050557/1‑57 (MQ=255) cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc > 1:1299216/1‑65 (MQ=255) gcgATACATGACATCCCTTATCTGATTGCCAAAAAGAGCAACTATCCCCATGCCGACGCTATTCATAcggc > 1:2421094/1‑71 (MQ=255) gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:576693/46‑1 (MQ=255) tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc < 1:3118730/67‑1 (MQ=255) cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg < 1:811494/52‑1 (MQ=255) cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg < 1:1127274/52‑1 (MQ=255) cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg < 1:2316378/52‑1 (MQ=255) cATGACGTCCCTTATCTGATTGCCAAAAAGCGCAACCATcc < 1:3461658/41‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc < 1:3715975/42‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg > 1:3457701/1‑66 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCt < 1:2381209/70‑1 (MQ=255) cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg > 1:4990284/1‑71 (MQ=255) aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:2625883/47‑1 (MQ=255) aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc < 1:4485198/47‑1 (MQ=255) aTCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa < 1:4898262/49‑1 (MQ=255) tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa < 1:700276/48‑1 (MQ=255) tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc < 1:4346774/71‑1 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg < 1:1170258/37‑1 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:2946378/1‑41 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt > 1:4697492/1‑51 (MQ=255) ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt > 1:2166381/1‑71 (MQ=255) cTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg > 1:4444284/1‑71 (MQ=255) ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:1282397/1‑44 (MQ=255) ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa > 1:649719/1‑44 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:3921082/1‑38 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa > 1:1926275/1‑38 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc > 1:1708686/1‑46 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc > 1:4624941/1‑46 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg < 1:3690337/60‑1 (MQ=255) tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt > 1:3801171/1‑71 (MQ=255) accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt > 1:3829765/3‑70 (MQ=255) accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt > 1:4720228/3‑70 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa > 1:1366089/1‑46 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt > 1:5463544/1‑70 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt < 1:1084463/70‑1 (MQ=255) aTCTGATTGCCAAAAAGCGCAACCATCCCAATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt > 1:1190558/1‑70 (MQ=255) tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt < 1:3732014/69‑1 (MQ=255) ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGa > 1:831240/2‑36 (MQ=16) ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt < 1:3537745/68‑1 (MQ=255) cTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg < 1:5044695/66‑1 (MQ=255) cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCttt < 1:2727207/67‑3 (MQ=255) cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc < 1:1573822/71‑1 (MQ=255) tGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt < 1:4778394/41‑1 (MQ=255) tGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt > 1:2862651/1‑40 (MQ=255) tGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTAct > 1:4354769/1‑71 (MQ=255) aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct > 1:2202953/1‑71 (MQ=255) aTTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCa > 1:5217061/1‑41 (MQ=19) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg < 1:4366441/71‑1 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg > 1:1663345/1‑71 (MQ=255) tGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg > 1:992449/1‑70 (MQ=255) tGCCAAAAAACGTAATCATCCTCATGCCGACGCCAtt > 1:2111344/1‑37 (MQ=16) cAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATg > 1:4926543/1‑71 (MQ=255) aaaaaaCGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt > 1:2334898/1‑71 (MQ=255) aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt > 1:3214145/1‑71 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCAt < 1:2710445/35‑1 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGTCTGGGTGAGCCTGTTTACTCTGCATGTTAt > 1:308777/1‑71 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg > 1:780185/1‑45 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt > 1:1535867/1‑71 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt > 1:1164799/1‑71 (MQ=255) aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:22948/71‑1 (MQ=255) aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:5297502/71‑1 (MQ=255) gcgcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCt < 1:79918/71‑1 (MQ=255) ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt < 1:2571623/71‑1 (MQ=255) ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt < 1:660972/71‑1 (MQ=255) ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt < 1:5276981/71‑1 (MQ=255) ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt < 1:515595/71‑1 (MQ=255) | TCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGT > minE/2569771‑2569911 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |