| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,892 | T→G | 27.6% | T54T (ACT→ACG) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,892 | 0 | T | G | 27.6% | 160.3 / 104.4 | 134 | T54T (ACT→ACG) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (52/45); new base G (17/20); total (69/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 140 total aligned reads displayed.
TGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTA > minE/2569822‑2569959 | tGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTAct > 1:4354769/1‑71 (MQ=255) aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct > 1:2202953/1‑71 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct > 1:3853554/1‑70 (MQ=255) ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg < 1:4366441/71‑1 (MQ=255) tGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg > 1:992449/1‑70 (MQ=255) cAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATg > 1:4926543/1‑71 (MQ=255) aaaaaaCGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt > 1:2334898/1‑71 (MQ=255) aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt > 1:3214145/1‑71 (MQ=255) aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt > 1:435764/1‑70 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGTCTGGGTGAGCCTGTTTACTCTGCATGTTAt > 1:308777/1‑71 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt > 1:1164799/1‑71 (MQ=255) aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt > 1:1535867/1‑71 (MQ=255) aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:5297502/71‑1 (MQ=255) aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:22948/71‑1 (MQ=255) gcgcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCt < 1:79918/71‑1 (MQ=255) cgcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTg > 1:4147305/1‑71 (MQ=255) aaCCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:3414335/66‑1 (MQ=255) tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa < 1:1396186/61‑1 (MQ=255) tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTAt > 1:3518308/2‑63 (MQ=255) tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGt < 1:1009023/70‑1 (MQ=255) ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt < 1:660972/71‑1 (MQ=255) ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt < 1:2571623/71‑1 (MQ=255) ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt < 1:5276981/71‑1 (MQ=255) cATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt > 1:2114441/1‑59 (MQ=255) aTCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGtt > 1:5651686/1‑59 (MQ=255) aTCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGtt > 1:3210049/1‑59 (MQ=255) aTCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGttt < 1:5020467/71‑1 (MQ=255) tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGttt < 1:2742562/70‑1 (MQ=255) tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc < 1:1489890/71‑1 (MQ=255) cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtg < 1:2724093/71‑1 (MQ=255) cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt < 1:3899699/70‑1 (MQ=255) cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt > 1:4563581/1‑70 (MQ=255) tcATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa < 1:2215821/55‑1 (MQ=255) tcATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGttttt < 1:3591591/68‑3 (MQ=255) ccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt < 1:3216579/55‑1 (MQ=255) cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt < 1:1049063/68‑1 (MQ=255) cATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgtg > 1:4692436/1‑71 (MQ=255) cATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgtg > 1:2941559/1‑71 (MQ=255) aTGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg < 1:5633290/68‑1 (MQ=255) aTGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTgg < 1:1260818/71‑1 (MQ=255) tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTAct < 1:3876431/43‑1 (MQ=255) tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGa < 1:5629547/71‑1 (MQ=255) tGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGa < 1:1399860/71‑1 (MQ=255) gCCGACGCTATTCATACGGCTGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGa < 1:469848/70‑1 (MQ=255) gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGttttt > 1:1053594/1‑63 (MQ=255) gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg > 1:1212457/1‑66 (MQ=255) gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg > 1:5564650/1‑66 (MQ=255) gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGAt > 1:5610432/1‑71 (MQ=255) ccGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGAt > 1:1878292/1‑70 (MQ=255) gACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg < 1:912649/71‑1 (MQ=255) cGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTggg < 1:3254356/71‑1 (MQ=255) ttCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt < 1:4987888/41‑1 (MQ=255) tCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc < 1:5308913/43‑1 (MQ=255) tCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTgg > 1:1897592/1‑58 (MQ=255) cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACg > 1:4651877/1‑69 (MQ=255) cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGc > 1:876759/1‑70 (MQ=255) aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt < 1:4225046/38‑1 (MQ=255) aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt < 1:35103/38‑1 (MQ=255) aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc < 1:17661/71‑1 (MQ=255) aCGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc < 1:49556/49‑1 (MQ=255) aCGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg > 1:4947383/1‑59 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGcc > 1:2241659/1‑43 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGcc > 1:5406819/1‑43 (MQ=255) cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGcc > 1:4706747/1‑43 (MQ=255) gcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGcca > 1:4891553/1‑59 (MQ=255) gttGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgtg < 1:1668426/44‑1 (MQ=255) gttGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTa > 1:5410307/3‑64 (MQ=255) gttGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTa > 1:4930081/3‑64 (MQ=255) gCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa > 1:1167625/1‑57 (MQ=255) gCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCg < 1:3910979/71‑1 (MQ=255) gCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGAGCGACg < 1:734327/59‑1 (MQ=255) ttGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgtg < 1:1205255/44‑1 (MQ=255) gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTgg < 1:4455985/44‑1 (MQ=255) gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAg > 1:5049589/1‑71 (MQ=255) gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAg > 1:2167260/1‑71 (MQ=255) ggTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAg < 1:408269/70‑1 (MQ=255) tGAGTCTTTTTACGTTACATGTTATCTGGCCGttt < 1:3095838/35‑1 (MQ=16) gAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGAtt < 1:4228381/43‑2 (MQ=255) gAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCggg > 1:2862595/1‑69 (MQ=255) gAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTg > 1:2055722/1‑71 (MQ=255) aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:24931/1‑38 (MQ=255) aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtgtg > 1:2650144/1‑38 (MQ=255) aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTg < 1:179309/70‑1 (MQ=255) tGTTTAGTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgg > 1:738884/1‑71 (MQ=255) tGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTg < 1:264557/70‑1 (MQ=255) gTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc > 1:4858061/1‑37 (MQ=255) gTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATc > 1:2290054/1‑37 (MQ=255) gTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTggg > 1:4756302/1‑71 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgggg < 1:688267/71‑1 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgggg < 1:3236960/71‑1 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgggg < 1:2954639/71‑1 (MQ=255) ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa > 1:1533267/1‑42 (MQ=255) ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa > 1:1318107/1‑42 (MQ=255) ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa > 1:1296281/1‑42 (MQ=255) ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa > 1:3554903/1‑42 (MQ=255) ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGc > 1:692925/1‑60 (MQ=255) ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGc > 1:3863784/1‑60 (MQ=255) tACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAAc > 1:976676/1‑53 (MQ=255) tACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTa > 1:4382838/1‑71 (MQ=255) ctctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCgg < 1:4119199/54‑1 (MQ=255) | TGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTA > minE/2569822‑2569959 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |