| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,934 | C→T | 16.4% | L68L (CTC→CTT) | yibI → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,934 | 0 | C | T | 16.4% | 184.2 / 24.3 | 79 | L68L (CTC→CTT) | yibI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (25/41); new base T (8/5); total (33/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
ATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAGCGACTGACCC > minE/2569864‑2569999 | aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc < 1:17661/71‑1 (MQ=255) gttGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTa > 1:4930081/3‑64 (MQ=255) gttGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTa > 1:5410307/3‑64 (MQ=255) gCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCg < 1:3910979/71‑1 (MQ=255) gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAg > 1:2167260/1‑71 (MQ=255) gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAg > 1:5049589/1‑71 (MQ=255) ggTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAg < 1:408269/70‑1 (MQ=255) gAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCggg > 1:2862595/1‑69 (MQ=255) gAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTg > 1:2055722/1‑71 (MQ=255) aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTgg > 1:1817937/1‑71 (MQ=255) aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTg < 1:179309/70‑1 (MQ=255) aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTg < 1:4988941/70‑1 (MQ=255) tGTTTAGTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgg > 1:738884/1‑71 (MQ=255) tGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTg < 1:264557/70‑1 (MQ=255) gTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGt < 1:3236798/64‑1 (MQ=255) gTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTggg > 1:4756302/1‑71 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgggg < 1:688267/71‑1 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgggg < 1:3236960/71‑1 (MQ=255) tttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgggg < 1:2954639/71‑1 (MQ=255) ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGc > 1:692925/1‑60 (MQ=255) ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGc > 1:4911116/1‑60 (MQ=255) ttACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGc > 1:3863784/1‑60 (MQ=255) tACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAAc > 1:4468382/1‑53 (MQ=255) tACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAAc > 1:976676/1‑53 (MQ=255) tACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTggg > 1:2295864/1‑68 (MQ=255) tACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTa > 1:4382838/1‑71 (MQ=255) ctctGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCgg < 1:4119199/54‑1 (MQ=255) ttacATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCGGGGATGGGGAATGCa < 1:985011/68‑1 (MQ=255) tacATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCgggg < 1:600294/56‑1 (MQ=255) tGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTa < 1:4915046/43‑1 (MQ=255) acATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCGGGGATGGGGAATGCa > 1:2574627/2‑69 (MQ=255) acATGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCGGGGATGGGGAATGCa > 1:1599532/2‑69 (MQ=255) cATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGt < 1:2119049/70‑1 (MQ=255) tGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAg < 1:318272/49‑1 (MQ=16) tGTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAg < 1:34380/49‑1 (MQ=16) tGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAAcc < 1:4021559/45‑1 (MQ=255) tGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTcac < 1:3253850/71‑1 (MQ=255) tGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTcac < 1:1913031/71‑1 (MQ=255) gTTATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCGGGGATgg > 1:2496781/1‑57 (MQ=255) gTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTca > 1:4231000/1‑69 (MQ=255) ttATCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGa > 1:999846/1‑46 (MQ=16) ttATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTa > 1:2835761/1‑60 (MQ=255) ttATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACAt < 1:1248647/71‑1 (MQ=255) tATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTggg > 1:4415900/1‑56 (MQ=255) tATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATg > 1:5597047/1‑71 (MQ=255) aTCTGGCCGTTTTTGTGGATTTGGGCCACGCTTTACCGCCCGGAGCgggga > 1:2988230/1‑50 (MQ=255) aTCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGc < 1:468464/61‑1 (MQ=255) ggCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATg < 1:1051868/66‑1 (MQ=255) ggCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATg < 1:148293/66‑1 (MQ=255) ggCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATg < 1:614750/66‑1 (MQ=255) ggCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATg < 1:4453950/66‑1 (MQ=255) ggCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATg < 1:638176/66‑1 (MQ=255) ggCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCg > 1:3336548/1‑71 (MQ=255) gCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGtt < 1:4769139/67‑1 (MQ=255) gCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGt < 1:1753047/71‑1 (MQ=255) cGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCg < 1:4391573/71‑1 (MQ=255) cGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCg < 1:3495332/71‑1 (MQ=255) gTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGc < 1:505330/71‑1 (MQ=255) tttCTGTGGATCTGGGCGACGCTCTATCAACCGGa < 1:1133980/35‑1 (MQ=255) tttCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTg < 1:718837/44‑1 (MQ=255) tttCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTAt < 1:2822988/50‑1 (MQ=255) ttCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTgggg < 1:3754158/46‑1 (MQ=255) tCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGt > 1:5377266/1‑37 (MQ=255) tCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTg < 1:1495127/38‑1 (MQ=255) tCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGc > 1:5674379/1‑40 (MQ=255) tCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTcac > 1:3816473/1‑56 (MQ=255) tCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAg > 1:578893/1‑70 (MQ=255) gtgGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGgag > 1:5551162/1‑70 (MQ=255) tgGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGga < 1:3482460/68‑1 (MQ=255) tgGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGaa < 1:3762746/71‑1 (MQ=255) ggATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGt < 1:5142237/41‑1 (MQ=255) ggATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGaa < 1:3769422/70‑1 (MQ=255) gATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAg > 1:145935/1‑71 (MQ=255) aTCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTAt < 1:4576953/41‑1 (MQ=255) aTCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTAt < 1:55658/41‑1 (MQ=255) cTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGt > 1:4983688/1‑56 (MQ=255) ggCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAGc < 1:1425475/66‑1 (MQ=255) ggCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAGc < 1:789601/66‑1 (MQ=255) ggCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAGCg < 1:2495885/67‑1 (MQ=255) aCGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAGCGACTGAccc < 1:143264/71‑1 (MQ=255) | ATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGGCTGGGGTATGCAGTCACATGTTGCGTCGCAGGAGAAAGCGACTGACCC > minE/2569864‑2569999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |