| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,798 | G→A | 13.9% | L359L (CTG→TTG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,798 | 0 | G | A | 13.9% | 331.5 / 32.3 | 122 | L359L (CTG→TTG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (39/66); new base A (11/6); total (50/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.74e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
ACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGA > minE/311728‑311867 | aCGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAg > 1:612465/1‑71 (MQ=255)aCGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAg > 1:3481233/1‑71 (MQ=255) ggCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGa < 1:3631523/71‑1 (MQ=255) aaGGTCCCCAGCAGCACCACCGGTACGGCGATGGTCGGGATCAGCGTGGCGCGGAAGTTCTGCAGGAACAg > 1:2637157/1‑71 (MQ=11) aaGGTCCCCAGCAGCACCACCGGTACGGCGATGGTCGGGATCAGCGTGGCGCGGAAGTTCTGCAGGAACAg > 1:5237249/1‑71 (MQ=11) aaGGTCCCCAGCAGCACCACCGGTACGGCGATGGTCGGGATCAGCGTGGCGCGGAAGTTCTGCAGGAACAg > 1:5215938/1‑71 (MQ=11) ggTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGa > 1:1236659/1‑70 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa < 1:122985/64‑1 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACa < 1:3948426/70‑1 (MQ=255) cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAcatacata < 1:128063/71‑1 (MQ=255) cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa < 1:2566942/71‑1 (MQ=255) aacAACACCACGGGTACCGCAATGGTGGGGATCAGAGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:5222363/3‑71 (MQ=2) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:2780819/1‑59 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc < 1:2970212/71‑1 (MQ=255) gCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAcc < 1:1008852/71‑1 (MQ=255) acAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:1661494/2‑70 (MQ=2) aacaccacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAg > 1:4596896/4‑71 (MQ=2) aaTACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:511256/65‑1 (MQ=255) acaccacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAgg < 1:4670260/69‑1 (MQ=9) accacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAgg < 1:1106224/69‑1 (MQ=9) ccaccGGTACGGCAATGGTCGGATTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:4568543/1‑70 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt > 1:4393492/1‑61 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAac < 1:403968/71‑1 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGAAGGAACAg > 1:2907685/1‑55 (MQ=255) caccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc < 1:5398554/52‑1 (MQ=255) acgggTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAg > 1:3124164/4‑65 (MQ=2) acgggTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAacac < 1:5493422/68‑1 (MQ=9) accGGTACGGCAATGGTCGGAATCAACTTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac < 1:4566776/71‑1 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:4882378/66‑1 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac < 1:3584368/71‑1 (MQ=255) ccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAg > 1:266931/1‑52 (MQ=255) ccGGTACGGCAATGGTAGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa > 1:4796944/1‑51 (MQ=255) ggTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg > 1:1448485/1‑71 (MQ=255) aCGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgat > 1:4248622/1‑71 (MQ=255) ccgCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGcat < 1:786931/68‑1 (MQ=9) cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:4147432/71‑1 (MQ=255) cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:109107/71‑1 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc > 1:2497879/1‑42 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAAc > 1:1191143/1‑42 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatgat < 1:1848565/71‑1 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatgat < 1:5089969/71‑1 (MQ=255) cAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAcc < 1:1768456/53‑1 (MQ=2) aaTGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGACCACCAGcataatg > 1:5560352/1‑69 (MQ=2) aaTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa > 1:2928644/1‑50 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:1387417/47‑1 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:1821335/47‑1 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa < 1:2646836/48‑1 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACg < 1:2180989/60‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:1701577/1‑37 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:3283076/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:3730336/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:1524122/61‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:2015780/61‑1 (MQ=255) ggTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg > 1:4168476/1‑60 (MQ=255) gTCGGATTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAg < 1:4376172/59‑1 (MQ=255) cGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGa > 1:1014996/1‑71 (MQ=255) aaTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCa < 1:5031656/71‑1 (MQ=255) aTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAg > 1:1071591/1‑71 (MQ=255) tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACg < 1:1713518/50‑1 (MQ=255) tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:1918509/56‑1 (MQ=255) tCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:2094437/56‑1 (MQ=255) aaCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:3908644/70‑1 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:4869205/40‑1 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt > 1:2748303/1‑71 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt > 1:2754883/1‑71 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt > 1:5371946/1‑71 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt > 1:1417996/1‑71 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:4426564/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:950230/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:4208913/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:1484380/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:3121986/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:3234591/71‑1 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:1937636/66‑1 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:3918965/71‑1 (MQ=255) tCGCGCGGAAGTTCTGAAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:2248828/66‑1 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:1197818/1‑70 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:3370189/1‑70 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc < 1:2932654/71‑1 (MQ=255) cgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc < 1:4399059/71‑1 (MQ=255) ggAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACt > 1:3698759/1‑71 (MQ=255) gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAgg < 1:3898201/40‑1 (MQ=255) gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAgg < 1:1332773/40‑1 (MQ=255) aGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGa > 1:3504734/1‑51 (MQ=255) aGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGa > 1:2479405/1‑51 (MQ=255) gTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGt > 1:3065788/1‑71 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCa < 1:4895700/52‑1 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCa < 1:5266851/52‑1 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:5126597/71‑1 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:4650009/71‑1 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:1448128/71‑1 (MQ=255) ttCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCg > 1:4140710/1‑48 (MQ=12) ttCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGt > 1:2819597/1‑70 (MQ=18) ttCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGt > 1:4502896/1‑70 (MQ=18) tCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg > 1:4024720/1‑70 (MQ=255) tCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTg > 1:3099907/1‑70 (MQ=18) cTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGc > 1:2516665/1‑52 (MQ=255) cTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGaa > 1:2686001/1‑71 (MQ=255) cAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAAt < 1:207952/69‑1 (MQ=255) aGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATaga < 1:836204/71‑1 (MQ=255) | ACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGA > minE/311728‑311867 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |