BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,570,821	T→G	8.5%	A242A (GCT→GCG)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,570,821	0	T	G	8.5%	260.8 / 17.8	106	A242A (GCT→GCG)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (40/57); new base G (6/3); total (46/60)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

Only 100 of 111 total aligned reads displayed.

AGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGG  >  minE/2570751‑2570890

aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                                       <  1:3225794/71‑1 (MQ=255)

aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                                       <  1:3218372/71‑1 (MQ=255)

aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                                                       <  1:428766/71‑1 (MQ=255)

 gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                      >  1:3098634/1‑71 (MQ=255)

      tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCtt                                                                  <  1:124460/70‑1 (MQ=255)

        aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                                               >  1:2726988/1‑71 (MQ=255)

         ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:5155473/71‑1 (MQ=255)

         ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:1277911/71‑1 (MQ=255)

         ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:3065483/71‑1 (MQ=255)

         ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:96965/71‑1 (MQ=255)

         ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:2016074/71‑1 (MQ=255)

         ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:3667828/71‑1 (MQ=255)

         ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:3303062/71‑1 (MQ=255)

         ttCGCGCGCCGAGCAAAGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              <  1:1593534/71‑1 (MQ=255)

          tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                             >  1:4405786/1‑71 (MQ=255)

           cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                                                              >  1:4819656/1‑69 (MQ=255)

               cgcCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGt                                                        >  1:4854762/1‑71 (MQ=255)

               cgcCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGt                                                        >  1:2187208/1‑71 (MQ=255)

                gcagAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                       >  1:5095792/4‑71 (MQ=255)

                gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                       >  1:5027913/1‑71 (MQ=255)

                   gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                                    <  1:4523482/71‑1 (MQ=255)

                   gAGCAATGGCTACGTTACTCAGGTACTGAACCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTcc                                                      >  1:2462052/1‑69 (MQ=255)

                    aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                      >  1:1658442/1‑52 (MQ=255)

                    aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                      >  1:3905850/1‑52 (MQ=255)

                    aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                      >  1:13360/1‑52 (MQ=255)

                     gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                                    <  1:3771478/69‑1 (MQ=255)

                     gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                                                    <  1:2791965/69‑1 (MQ=255)

                        aTGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATg                                               >  1:544441/1‑71 (MQ=255)

                        aTGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATg                                               <  1:4950043/71‑1 (MQ=255)

                        aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                       <  1:2249279/63‑1 (MQ=255)

                        aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                       <  1:5461260/63‑1 (MQ=255)

                         tGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATgg                                              >  1:622311/1‑71 (MQ=255)

                         tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa                                                 <  1:198856/68‑1 (MQ=255)

                          ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg                                                     >  1:4733665/1‑63 (MQ=255)

                          ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg                                                     >  1:951436/1‑63 (MQ=255)

                          ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg                                              >  1:2486290/1‑70 (MQ=255)

                          ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                                             >  1:4296935/1‑71 (MQ=255)

                          ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt                                             >  1:5129572/1‑71 (MQ=255)

                           gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                            >  1:3392954/1‑71 (MQ=255)

                            cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                                           <  1:4172809/71‑1 (MQ=255)

                               cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                          <  1:1319461/69‑1 (MQ=255)

                               cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                         <  1:4657486/70‑1 (MQ=255)

                               cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                                        >  1:2203160/1‑71 (MQ=255)

                                gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                                                               <  1:4329799/47‑1 (MQ=255)

                                 ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                             <  1:3557122/48‑1 (MQ=255)

                                 ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                                                       <  1:2937265/54‑1 (MQ=255)

                                   aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTg                                                           >  1:2121406/1‑48 (MQ=255)

                                   aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTg                                                           >  1:2601556/1‑48 (MQ=255)

                                   aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATcc                                     >  1:415778/1‑70 (MQ=255)

                                    cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                      <  1:879172/36‑1 (MQ=255)

                                    cTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                                                      <  1:3699059/36‑1 (MQ=255)

                                       aGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCg                                                     >  1:126606/1‑50 (MQ=255)

                                       aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                                                             >  1:4093893/1‑42 (MQ=255)

                                       aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                            >  1:4223055/1‑59 (MQ=255)

                                       aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                                >  1:2878984/1‑71 (MQ=255)

                                        ggTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                                >  1:3890918/1‑70 (MQ=255)

                                         gTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCa                              <  1:2235105/71‑1 (MQ=255)

                                         gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTg                                                  <  1:632709/51‑1 (MQ=255)

                                         gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                          <  1:2622058/59‑1 (MQ=255)

                                         gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                          <  1:261161/59‑1 (MQ=255)

                                         gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                          <  1:4955373/59‑1 (MQ=255)

                                           aCTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCag                             >  1:5457882/1‑69 (MQ=255)

                                            cTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCaga                            <  1:1211790/70‑3 (MQ=255)

                                            cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                            <  1:2417283/70‑1 (MQ=255)

                                            cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                            <  1:1549848/70‑1 (MQ=255)

                                            cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                            <  1:1625402/70‑1 (MQ=255)

                                              gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCa                              >  1:1108039/1‑66 (MQ=255)

                                               aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaaa                          <  1:5462102/69‑1 (MQ=255)

                                               aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAAc                         <  1:4349864/70‑1 (MQ=255)

                                                tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCAt                                       >  1:2717923/1‑55 (MQ=255)

                                                tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACgg                       >  1:4744885/1‑71 (MQ=255)

                                                  cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                     <  1:2631997/71‑1 (MQ=255)

                                                  cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                     <  1:2159368/71‑1 (MQ=255)

                                                    cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                     <  1:1483664/69‑1 (MQ=255)

                                                        ggTACATACGCAGCTGCCTTGCCGCTGCGTACGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGt               >  1:1046851/1‑71 (MQ=255)

                                                         gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa                           <  1:2733484/58‑1 (MQ=255)

                                                         gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTc              <  1:1125899/71‑1 (MQ=255)

                                                           aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                         >  1:1756262/1‑42 (MQ=255)

                                                           aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                                        >  1:1705886/1‑43 (MQ=255)

                                                           aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGc            >  1:4283257/1‑71 (MQ=255)

                                                            cATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                         <  1:600178/41‑1 (MQ=255)

                                                            cATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                         >  1:802483/1‑41 (MQ=255)

                                                              tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                         <  1:1082178/39‑1 (MQ=255)

                                                              tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                         <  1:396009/39‑1 (MQ=255)

                                                              tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGc                               <  1:2466572/49‑1 (MQ=255)

                                                              tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                            <  1:1697807/52‑1 (MQ=255)

                                                              tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                            <  1:5627644/52‑1 (MQ=255)

                                                               aCGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCaa        >  1:5118750/1‑71 (MQ=255)

                                                               aCGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCaa        >  1:3306124/1‑71 (MQ=255)

                                                                cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAAc                         <  1:4641982/53‑1 (MQ=255)

                                                                cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAt       >  1:4691553/1‑71 (MQ=255)

                                                                cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAt       >  1:2123252/1‑71 (MQ=255)

                                                                  cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt     >  1:1563921/1‑71 (MQ=255)

                                                                  cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt     <  1:2527354/71‑1 (MQ=255)

                                                                  cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt     <  1:2737867/71‑1 (MQ=255)

                                                                   aGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTc    <  1:5590133/71‑1 (MQ=255)

                                                                   aGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTc    <  1:447287/71‑1 (MQ=255)

                                                                     cTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTctg  <  1:5453895/71‑3 (MQ=255)

                                                                      tGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGa                                 >  1:3208350/1‑39 (MQ=255)

                                                                      tGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGa                                 >  1:3548325/1‑39 (MQ=255)

AGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGG  >  minE/2570751‑2570890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`