BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,570,848	C→G	9.9%	V251V (GTC→GTG)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,570,848	0	C	G	9.9%	285.7 / 17.0	101	V251V (GTC→GTG)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (40/51); new base G (4/6); total (44/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 107 total aligned reads displayed.

GCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTG  >  minE/2570778‑2570918

gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                                                        >  1:3392954/1‑71 (MQ=255)

gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                                                        <  1:2328269/71‑1 (MQ=255)

 cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                                                                       <  1:4172809/71‑1 (MQ=255)

    cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                                                      <  1:1319461/69‑1 (MQ=255)

    cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                                                     <  1:4657486/70‑1 (MQ=255)

    cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                                                                    >  1:2203160/1‑71 (MQ=255)

      ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc                                                                  >  1:508147/1‑71 (MQ=255)

        aCTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATcc                                                                 >  1:415778/1‑70 (MQ=255)

            aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc                                                                        >  1:4223055/1‑59 (MQ=255)

            aGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                                                            >  1:2878984/1‑71 (MQ=255)

             ggTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAg                                                            >  1:3890918/1‑70 (MQ=255)

              gTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCa                                                          <  1:2235105/71‑1 (MQ=255)

              gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                                                      <  1:261161/59‑1 (MQ=255)

              gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                                                      <  1:554302/59‑1 (MQ=255)

              gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                                                      <  1:2622058/59‑1 (MQ=255)

              gTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt                                                                      <  1:4955373/59‑1 (MQ=255)

                aCTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCag                                                         >  1:5457882/1‑69 (MQ=255)

                 cTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCaga                                                        <  1:1211790/70‑3 (MQ=255)

                 cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaa                                                       <  1:3691402/71‑1 (MQ=255)

                 cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                                                        <  1:2417283/70‑1 (MQ=255)

                 cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                                                        <  1:3172040/70‑1 (MQ=255)

                 cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                                                        <  1:1625402/70‑1 (MQ=255)

                 cTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                                                        <  1:1549848/70‑1 (MQ=255)

                   gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATccc                                                                >  1:1758223/1‑60 (MQ=255)

                   gATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCa                                                          >  1:1108039/1‑66 (MQ=255)

                    aTCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaaaa                                                      <  1:5462102/69‑1 (MQ=255)

                     tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCAt                                                                   >  1:2717923/1‑55 (MQ=255)

                     tCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACgg                                                   >  1:4744885/1‑71 (MQ=255)

                       cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                                                 <  1:2159368/71‑1 (MQ=255)

                       cGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                                                 <  1:2631997/71‑1 (MQ=255)

                         cccAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCa                                                 <  1:1483664/69‑1 (MQ=255)

                           cAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt                                                                       <  1:1420553/45‑1 (MQ=255)

                             ggTACATACGCAGCTGCCTTGCCGCTGCGTACGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGt                                           >  1:1046851/1‑71 (MQ=255)

                              gTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTc                                          <  1:1125899/71‑1 (MQ=255)

                                aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                                                     >  1:1756262/1‑42 (MQ=255)

                                aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa                                                                    >  1:1705886/1‑43 (MQ=255)

                                aCATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGc                                        >  1:4283257/1‑71 (MQ=255)

                                 cATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                                                     >  1:802483/1‑41 (MQ=255)

                                 cATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                                                     <  1:600178/41‑1 (MQ=255)

                                   tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                                                     <  1:1082178/39‑1 (MQ=255)

                                   tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc                                                                     <  1:396009/39‑1 (MQ=255)

                                   tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGc                                                           <  1:2466572/49‑1 (MQ=255)

                                   tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                                                        <  1:1697807/52‑1 (MQ=255)

                                   tACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                                                        <  1:5627644/52‑1 (MQ=255)

                                    aCGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCaa                                    >  1:3306124/1‑71 (MQ=255)

                                    aCGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCaa                                    >  1:5118750/1‑71 (MQ=255)

                                     cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAAc                                                     <  1:4641982/53‑1 (MQ=255)

                                     cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAt                                   >  1:4691553/1‑71 (MQ=255)

                                     cGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAt                                   >  1:2123252/1‑71 (MQ=255)

                                      gCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCaaa                                                        <  1:4283010/49‑1 (MQ=255)

                                       cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                 <  1:2737867/71‑1 (MQ=255)

                                       cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                 >  1:1563921/1‑71 (MQ=255)

                                       cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                 <  1:2527354/71‑1 (MQ=255)

                                        aGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTc                                <  1:5590133/71‑1 (MQ=255)

                                        aGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTc                                <  1:447287/71‑1 (MQ=255)

                                          cTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTctg                              <  1:5453895/71‑3 (MQ=255)

                                           tGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGa                                                             >  1:3208350/1‑39 (MQ=255)

                                           tGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGa                                                             >  1:3548325/1‑39 (MQ=255)

                                             ccTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCgg                              >  1:5018281/1‑68 (MQ=255)

                                             ccTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaa                           <  1:4999601/71‑1 (MQ=255)

                                             ccTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaa                           <  1:4446137/71‑1 (MQ=255)

                                              gctGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAAc                                                     >  1:3872822/3‑44 (MQ=12)

                                              gctGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCag                           <  1:729192/68‑2 (MQ=255)

                                              gctGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCag                           <  1:4087924/68‑2 (MQ=255)

                                                tGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCTAAAACGGCAAATTGTCGCCCAAttt                                 >  1:2746410/1‑62 (MQ=255)

                                                tGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                 >  1:3171260/1‑62 (MQ=255)

                                                tGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                 >  1:1634008/1‑62 (MQ=255)

                                                tGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaaaa                         >  1:4206615/1‑70 (MQ=255)

                                                 gCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGt                                           >  1:2903263/1‑51 (MQ=255)

                                                 gCCGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCAGAACt                       >  1:1614448/1‑71 (MQ=255)

                                                  ccGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTc                      <  1:1531971/71‑1 (MQ=255)

                                                  ccGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCAGAACTc                      >  1:352963/1‑71 (MQ=255)

                                                   cGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCAGAACTcc                     <  1:1330329/71‑2 (MQ=255)

                                                    gCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAAtt                                             <  1:1895200/46‑1 (MQ=255)

                                                    gCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGc                    <  1:774436/71‑1 (MQ=255)

                                                     cTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCt                   >  1:3564061/1‑71 (MQ=255)

                                                      tacGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCag                           <  1:1854955/60‑2 (MQ=255)

                                                        cGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCg                     <  1:2480910/66‑1 (MQ=255)

                                                        cGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCg                     <  1:4251836/66‑1 (MQ=255)

                                                        cGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCg                     <  1:3251621/66‑1 (MQ=255)

                                                          tCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTAc              <  1:3943/71‑1 (MQ=255)

                                                           ccGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTAc              <  1:5338924/70‑1 (MQ=255)

                                                            cGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGt            <  1:2256062/71‑1 (MQ=255)

                                                            cGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGt            >  1:406426/1‑71 (MQ=255)

                                                              gTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCt          <  1:5055378/71‑1 (MQ=255)

                                                              gTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCt          <  1:2653371/71‑1 (MQ=255)

                                                               tGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGc                             <  1:2582970/51‑1 (MQ=255)

                                                               tGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCt          >  1:3074010/1‑70 (MQ=255)

                                                               tGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTg         <  1:5162461/71‑1 (MQ=255)

                                                                 aTGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaa                           >  1:155038/1‑51 (MQ=255)

                                                                 aTGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGa        <  1:2546995/70‑1 (MQ=255)

                                                                  tGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAt                                   <  1:1138752/42‑1 (MQ=255)

                                                                  tGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGaaa      >  1:3445468/1‑71 (MQ=255)

                                                                   ggTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAAc     >  1:964811/1‑71 (MQ=255)

                                                                   ggTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAAc     >  1:852979/1‑71 (MQ=255)

                                                                   ggTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAAc     >  1:1499124/1‑71 (MQ=255)

                                                                    gTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAAcc    <  1:4823359/71‑1 (MQ=255)

                                                                      cTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaaaa                         >  1:211175/1‑48 (MQ=255)

                                                                      cTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTg  >  1:5380065/1‑71 (MQ=255)

                                                                      cTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTg  >  1:1113757/1‑71 (MQ=255)

GCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTG  >  minE/2570778‑2570918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`